Effect of increased vegetable and fruit consumption on markers of oxidative cellular damage

Abstract

The goal of this study was to test the hypothesis that increased consumption of vegetables and fruit would reduce markers of oxidative cellular damage that can be assessed in blood or urine. Twenty-eight women participated in a 14 day dietary intervention. The primary end-points assessed were: 8-hydroxydeoxyguanosine (8-OHdG) in DNA isolated from peripheral lymphocytes, determined by HPLC with electrochemical detection; 8-OHdG excreted in urine, measured by ELISA; malondialdehyde (MDA) in urine, measured by fluorimetric detection following derivatization with thiobarituric acid and separation via HPLC; urinary 8-isoprostane F-2alpha (8-EPG) detected by ELISA. Pre- and post-intervention plasma levels of selected carotenoids were determined by HPLC. Subjects were free living and consumed a completely defined recipe-based diet that increased their average daily consumption of vegetables and fruit from 5.8 servings at baseline to 12.0 servings throughout the intervention. Overall, the level of 8-OHdG in DNA isolated from lymphocytes and in urine and the level of 8-EPG in urine were reduced by the intervention, whereas urine concentrations of MDA were minimally affected. The reduction in lymphocyte 8-OHdG was greater in magnitude (32 versus 5%) in individuals with lower average pre-intervention levels of plasma alpha-carotene (56 ng/ml) than in individuals with higher average pre-intervention plasma levels of alpha-carotene (148 ng/ml). The results of this study indicate that consumption of a diet that significantly increased vegetable and fruit intake from a diverse number of botanical families resulted in significant reductions in markers of oxidative cellular damage to DNA and lipids.

Plasma and Dietary Carotenoids Are Associated with Reduced Oxidative Stress in Women Previously Treated for Breast Cancer

“…Our findings suggesting that increased intake of fruits and vegetables is associated with reduced oxidative stress are supported by some controlled feeding trials (20) but not others (38,39). In a study conducted among 28 women assessed to be at greater risk for breast cancer than the general population, Thompson et al (20) showed a significant inverse relationship between increased fruit and vegetable intake during a 14-day intervention and change in urinary 8-iso-PGF2a.…”

“…Higher intake of carotenoid-rich fruit and vegetables has been associated with a significant reduction in oxidative stress biomarkers, both in relation to DNA damage and lipid peroxidation (18)(19)(20). Diets low in antioxidants, particularly antioxidant carotenoids provided as vegetables and fruit in the diet, may contribute to increased oxidative stress (20,21) and thus indirectly contribute to breast cancer recurrence.…”

“…Diets low in antioxidants, particularly antioxidant carotenoids provided as vegetables and fruit in the diet, may contribute to increased oxidative stress (20,21) and thus indirectly contribute to breast cancer recurrence.…”

Calorie Restricted Diet and Urinary Pentosidine in Patients with Rheumatoid Arthritis

“…Further, the foodstuffs used in this study were rich in antioxidant. It has been reported that a diet antioxidant and fruites may have an antioxidant effects (Fan WY et al, 2000;Pool-Zobel et al, 1997;Thompson et al, 1999;Verhagen et al, 1997). A complex combination of these mechanisms might relate to the present results.…”

Assessment of Major Nutrient Contribution on Growth and Yield of Soybean (Glycine max L. Merril) in North Bihar, India

“…Bihar has notified forest area of 6,764.14 sq km, which is 7.1% of its geographical area (Department of Agriculture Gov of Bihar). Popular advice for healthy diets that may promote health and longevity include the daily consumption of at least three servings of fruits or vegetables and the variation of foods which are derived from different plants belonging to different botanical families (Thompson et al, 1999). Since, 2012 introduction of soybean in Begusarai district led to a shift in cropping system during rainy season from rice -wheat, rice -maize, maize -maize to soybeanwheat, soybean -maize system.…”