Effect of increased load of high-strength food wastewater in thermophilic and mesophilic anaerobic co-digestion of waste activated sludge on bacterial community structure

Jang, Hyun Min; Ha, Jeong Hyub; Kim, Mi-Sun; Kim, Jong‐Oh; Kim, Young Mo; Park, Jong

doi:10.1016/j.watres.2016.04.051

Cited by 102 publications

(24 citation statements)

References 47 publications

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“…In comparison, digester B was continuously dominated by the phylum Thermotogae (89% at 80 rpm, 87% at 50 rpm and 85% at 160 rpm), followed by Synergistetes (average of 6%) and Firmicutes (average of 5%) ( Figure 4.b). Similar Thermotogae dominance has been reported in thermophilic digesters treating organic solid waste, food waste, food wastewater and sugar beet tailings (Jang et al, 2016;Guo et al, 2014;Tian et al, 2013;Sasaki et al, 2011). The reported Thermotogae phyla were often dominated by Petrotoga, Defluviitoga or Fervidobacterium genera (Jang et al, 2016;Guo et al, 2014).…”

Section: Microbial Analysissupporting

confidence: 75%

“…Similar Thermotogae dominance has been reported in thermophilic digesters treating organic solid waste, food waste, food wastewater and sugar beet tailings (Jang et al, 2016;Guo et al, 2014;Tian et al, 2013;Sasaki et al, 2011). The reported Thermotogae phyla were often dominated by Petrotoga, Defluviitoga or Fervidobacterium genera (Jang et al, 2016;Guo et al, 2014). For further identification of the bacteria residing in the digesters, genus level affiliations were determined with the results showing that the Thermotogae phylum consisted mainly of one genus: Petrotoga, with a negligible presence (< 1%) of the Thermopallium genus.…”

Section: Microbial Analysissupporting

confidence: 59%

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Correlation between system performance and bacterial composition under varied mixing intensity in thermophilic anaerobic digestion of food waste

Ghanimeh

Al-Sanioura

Saikaly

et al. 2018

Journal of Environmental Management

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This study examines the stability and efficiency of thermophilic anaerobic digesters treating food waste under various mixing velocities (50-160 rpm). The results showed that high velocities (120 and 160 rpm) were harmful to the digestion process with 18-30% reduction in methane generation and 1.8 to 3.8 times increase in volatile fatty acids (VFA) concentrations, compared to mild mixing (50 and 80 rpm). Also, the removal rate of soluble COD dropped from 75 to 85% (at 50-80 rpm) to 20-59% (at 120-160 rpm). Similarly, interrupted mixing caused adverse impacts and led to near-failure conditions with excessive VFA accumulation (15.6 g l), negative removal rate of soluble COD and low methane generation (132 ml gVS). The best efficiency and stability were achieved under mild mixing (50 and 80 rpm). In particular, the 50 rpm stirring speed resulted in the highest methane generation (573 ml gVS). High-throughput sequencing of 16S rRNA genes revealed that the digesters were dominated by one bacterial genus (Petrotoga; phylym Thermotogae) at all mixing velocities except at 0 rpm, where the community was dominated by one bacterial genus (Anaerobaculum; phylum Synergistetes). The Petrotoga genus seems to have played a major role in the degradation of organic matter.

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Section: Microbial Analysissupporting

confidence: 75%

Section: Microbial Analysissupporting

confidence: 59%

Correlation between system performance and bacterial composition under varied mixing intensity in thermophilic anaerobic digestion of food waste

Ghanimeh

Al-Sanioura

Saikaly

et al. 2018

Journal of Environmental Management

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“…The initial strain richness and evenness were significantly lower in the thermophilic than in the mesophilic microbial communities, which are in line with other studies (Levén et al ., ; Guo et al ., ; Jang et al ., ). We observed declining trends for these indices in all mesophilic and thermophilic high‐acetate chemostats throughout operation, which was expected as feeding a restricted number of substrates limits the potential metabolic pathways used for microbial growth and thus likely affects the phylogenetic distribution.…”

Section: Discussionmentioning

confidence: 97%

Detection of novel syntrophic acetate‐oxidizing bacteria from biogas processes by continuous acetate enrichment approaches

Westerholm

Müller

Singh

et al. 2017

Microbial Biotechnology

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SummaryTo enrich syntrophic acetate‐oxidizing bacteria (SAOB), duplicate chemostats were inoculated with sludge from syntrophic acetate oxidation (SAO)‐dominated systems and continuously supplied with acetate (0.4 or 7.5 g l−1) at high‐ammonia levels. The chemostats were operated under mesophilic (37°C) or thermophilic (52°C) temperature for about six hydraulic retention times (HRT 28 days) and were sampled over time. Irrespective of temperature, a methane content of 64–69% and effluent acetate level of 0.4–1.0 g l−1 were recorded in chemostats fed high acetate. Low methane production in the low‐acetate chemostats indicated that the substrate supply was below the threshold for methanization of acetate via SAO. Novel representatives within the family Clostridiales and genus Syntrophaceticus (class Clostridia) were identified to represent putative SAOB candidates in mesophilic and thermophilic conditions respectively. Known SAOB persisted at low relative abundance in all chemostats. The hydrogenotrophic methanogens Methanoculleus bourgensis (mesophilic) and Methanothermobacter thermautotrophicus (thermophilic) dominated archaeal communities in the high‐acetate chemostats. In line with the restricted methane production in the low‐acetate chemostats, methanogens persisted at considerably lower abundance in these chemostats. These findings strongly indicate involvement in SAO and tolerance to high ammonia levels of the species identified here, and have implications for understanding community function in stressed anaerobic processes.

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“…However, details of the bacterial populations within those phyla and their ecological roles in the digestion process are yet to be determined. Moreover, the composition of the associated bacterial/methanogenic archaeal community seems to be influenced by process parameters such as temperature, pH and SRT . Therefore, it is valuable to explore the variations in microbial community structure in response to the process parameters during anaerobic digestion of sludge and provide some valid insight into possibly deteriorated performance.…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, the composition of the associated bacterial/methanogenic archaeal community seems to be influenced by process parameters such as temperature, pH and SRT. [25][26][27] Therefore, it is valuable to explore the variations in microbial community structure in response to the process parameters during anaerobic digestion of sludge and provide some valid insight into possibly deteriorated performance. There are several methods to investigate the microbial community in the environmental samples (soil, sludge and wastewater), including culture-based techniques, conventional microscopy, 4 ′ ,6-diamidino-2-phenylindole (DAPI) epifluorescence microscopy and conventional molecular techniques.…”

Section: Introductionmentioning

confidence: 99%

Microbial population dynamics in temperature‐phased anaerobic digestion of municipal wastewater sludge

Hameed

Riffat

et al. 2019

J of Chemical Tech & Biotech

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BACKGROUND The present study compared the performance and capability of thermophilic–mesophilic, temperature‐phased anaerobic digestion (TPAD) of wastewater sludge at 45 and 55 °C in terms of rate of hydrolysis and methanogenesis at two point interval times – December and January – with operational conditions kept constant (total solid content 6.5%, solids retention time 12.5 days). RESULTS The reduction of volatile solids (VS) was 77% at 45 °C TPAD‐1 and 72% at 55 °C TPAD‐II. The accumulation of ammonia and volatile fatty acids (VFAs; propionic acid) were observed to be below the inhibitory range (>3000 mg L−1), whereas the methane (CH4) production (45 °C, 3.55 ± 0.47 L CH4 L−1 day−1; 35 °C, 1.44 ± 0.12 L CH4 L−1 day−1) remained considerably higher in TPAD‐I than TPAD‐II. Furthermore, the TPAD‐II system suffered from certain degree of instability due to high level of total VFAs (6087 ± 1578 mg L−1), low buffering capacity, increased level of total NH3 (2982 ± 219 mg L−1) and free NH3 (246 ± 25 mg L−1), and relatively reduced CH4 production (1.69 ± 0.1 L L−1 day). The bacterial and archaeal population of the TPADs investigated by 454 pyrosequencing and Illumina sequencing showed, in both TPAD systems, a bacterial community dominated by Firmicutes, followed by Bacteriodetes, Proteobacteria, Synergistetes and Actiniobacteria. The archaeal community was dominated by Methanimicrobia (74–84% Methanosarcina) and Methanobacteria (15–27% Methanobacterium). A progression from genus Clostridium to Coprothermobacter and Tepidanaerobacter, and Methanocarcina to Methanothermobacter and Methanobacterium was observed in TPAD‐II. CONCLUSIONS This study proved the processes driving the dynamics of key microbial population and its correlation with hydrolytic functionality of TPAD systems. © 2019 Society of Chemical Industry

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Effect of increased load of high-strength food wastewater in thermophilic and mesophilic anaerobic co-digestion of waste activated sludge on bacterial community structure

Cited by 102 publications

References 47 publications

Correlation between system performance and bacterial composition under varied mixing intensity in thermophilic anaerobic digestion of food waste

Correlation between system performance and bacterial composition under varied mixing intensity in thermophilic anaerobic digestion of food waste

Detection of novel syntrophic acetate‐oxidizing bacteria from biogas processes by continuous acetate enrichment approaches

Microbial population dynamics in temperature‐phased anaerobic digestion of municipal wastewater sludge

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