Effect of incoherent LED radiation on third-degree burning wounds in rats

Abstract

The main physiological characteristics in a burn process are the increase of the capillary permeability and the occurrence of edema and exudation. Light-emitting diode (LED) has been proposed as treatment of burning. This study investigated the effects of LED on the repair process of rat skin submitted to a third-degree burning. The lesions were produced on the dorsal surface of male Wistar rats. Animals were divided into 4 groups (n = 6) as follows: L1 and L2 groups as LED-treated burned rats, and received LED therapy along 7 and 15 days with 48 hours intervals, respectively; C1 and C2 groups as control, non-treated burned rats. A red LED (640 nm, 30 mW) operating with a fluence of 4 J/cm(2) was used. The wound area was measured daily after irradiation. Animals were euthanized at the 8th and 16th days after burning, and the wound fragment was submitted to histology. The inflammatory cells as well as the damaged area at the 8th day after burns were significantly lower for the LED-treated group when compared to control. Furthermore, the LED phototherapy effect on cellular migration was even more pronounced at the 16th day. Our results indicated that the treatment with a LED system was clearly effective in reducing the number of inflammatory cells and improving the healing process in an experimental model of third-degree burnings.

630 nm Light Emitting Diode Irradiation Improves Dermal Wound Healing in Rats

“…Several studies have been reported that 630 nm and 640 nm LED irradia-tion with 4-24 J/cm 2 decreased wound surface area. 10,[15][16][17] Our results agree with the findings from several other studies that used 640 nm and 700 nm LED with 15-16 J/cm 2 to stimulate fibroblast proliferation and collagen production on excision wound in rats. 16,18,19 In the present study, epithelial gap of LED group showed signifi-…”

Comparative Study of Morphometric and Fourier Transform Infrared Spectroscopy Analyses of the Collagen Fibers in the Repair Process of Cutaneous Lesions

“…Considering the data obtained at this experimental time for the LED group, this result is probably due to the action of phototherapy, making energy available to the cells that were damaged by the surgical process, thus accelerating the repair process during its secondary phase. 20,21,25,43,44 The most significant results were observed with the combination of LED and a semipermeable dressing (LED + D). It is noteworthy that the application of the dressing positively influenced the tissue repair process by accelerating it.…”

Effect of LED therapy for the treatment nipple fissures

“…Breast milk is nutritionally ideal for the healthy development of a child and breastfeeding is ideal for orofacial growth and development. [ 8 , 13 ]…”

“…Phototherapy is the use of electromagnetic waves within the red and infrared spectra that are applied to biological tissues with the aid of low-level light devices, such as light amplification by stimulated emission of radiation (laser) and a light-emitting diode (LED). Studies have demonstrated the anti-inflammatory properties of phototherapy, with the enhancement of the wound repair process [ 11 – 13 ] and the control of pain. [ 14 , 15 ] As it does not cause discomfort, is relatively inexpensive, and may impede the discontinuation of breastfeeding, phototherapy could be a viable option for the treatment of nipple fissures.…”