Effect of implant surface microtopography by hydroxyapatite grit-blasting on adhesion, proliferation, and differentiation of osteoblast-like cell line, MG-63

Abstract

Objective: This study examined the potential of the in vitro osteogenesis of microtopographically modified surfaces, RBM (resorbable blasting media) surfaces, which generate hydroxyapatite grit-blasting. Methods: RBM surfaces were modified hydroxyapatite grit-blasting to produce microtopographically modified surfaces and the surface morphology, roughness or elements were examined. To investigate the potential of the in vitro osteogenesis, the osteoblastic cell adhesion, proliferation, and differentiation were examined using the human osteoblast-like cell line, MG-63 cells. Osteoblastic cell proliferation was examined as a function of time. In addition, osteoblastic cell differentiation was verified using four different methods of an ALP activity assay, a mineralization assay using alizarin red-s staining, and gene expression of osteoblastic differentiation marker using RT-PCR or ELISA. Results: Osteoblastic cell adhesion, proliferation and ALP activity was elevated on the RBM surfaces compared to the machined group. The cells exhibited a high level of gene expression of the osteoblastic differentiation makers (osteonectin, type I collagen, Runx-2, osterix). imilar data was represented in the ELISA produced similar results in that the RBM surface increased the level of osteocalcin, osteopontin, TGF-beta1 and PGE2 secretion, which was known to stimulate the osteogenesis. Moreover, alizarin red-s staining revealed significantly more mineralized nodules on the RBM surfaces than the machined discs. Conclusion: RBM surfaces modified with hydroxyapatite grit-blasting stimulate the in vitro osteogenesis of MG-63 cells and may accelerate bone formation and increase bone-implant contact.Key words: Osseointegration, Osteogenesis, Microtopography, Surface roughness, RBM (resorbable blasting media)[paper submitted 2011. 2. 8 / revised 2011. 5. 31 / accepted 2011. 6. 7] Abstract (J Korean Assoc Oral Maxillofac Surg 2011;37:214-24)

Tribocorrosion Behavior of Calcium- and Phosphorous-Enriched Titanium Oxide Films and Study of Osteoblast Interactions for Dental Implants

“…Cell adhesion is one of the initial critical stages to subsequent proliferation and differentiation [36,91,92] and in the present study it was addressed by SEM and CLSM imaging. MG63 cells were cultured on standard polystyrene culture wells (control), cp-Ti_Reference, PEO_CE,8,10, and PEO_Cβ,25,10 samples.…”

Porous nanoapatite scaffolds synthesized using an approach of interfacial mineralization reaction and their bioactivity

“…2,3,[14][15][16] In addition, surface microtopography has been reported to affect cell adhesion, morphology, proliferation, and differentiation. [17][18][19][20][21][22][23][24][25][26] Bowers et al 17 investigated osteoblast attachment on c.p. titanium discs and found higher cell attachment on the sand blasted discs compared with smoother surfaces.…”

“…Furthermore, some reports evaluated bone formation to implants with different surface roughness and found rougher implants showed enhanced bone formation compared with smoother implants. [19][20][21][22][23][24][25][26] Now, it is generally accepted that the structures of pores (porosity and pore size and interconnectivity) and the three-dimensional (3D) surface topography of pore walls are the most important parameters to influence cellular reactions and bone formation. Although many techniques have been used to fabricate porous calcium phosphate ceramic scaffolds, [27][28][29][30] there exist still difficulties in obtaining interconnected pores with very high porosity and also in engineering the topography of the pore walls for calcium phosphate ceramic scaffolds.…”

Antibacterial efficacy of triple-layered poly(lactic-<i>co</i>-glycolic acid)/nanoapatite/lauric acid guided bone regeneration membrane on periodontal bacteria

“…Significance differences between formulation of the antibacterial GBR membranes were determined through an analysis of variance (ANOVA) using SPSS v. 16.0 (SPSS, Chicago, IL, USA) statistical software at 95% confidence level (p<0.05). A statistical post-hoc analysis was also conducted through Tukey's multiple comparison test to evaluate differences between pair of data.…”

“…Nanoapatite (NAp) is commonly used as a nanofiller due to its excellent bioactive property with the resemblance to inorganic constituent of bone 14,15) . Its chemical composition, Ca10(PO4)6(OH)2, and rough morphological structure are the main factors that contribute to the acceleration of osteoblast cells attachment and proliferation 15,16) . Therefore, in this study, the GBR membranes obtained 17) where the L1 was incorporated with PLGA, the highest proportion of LA and the lowest proportion of NAp to accommodate the layer with an optimum antibacterial effect.…”