Effect of impaired kynurenine synthesis on memory in Drosophila

Никитина, Е. А.; Zhuravlev, Aleksandr V.; Савватеева-Попова, Е. В.

doi:10.33910/2687-1270-2021-2-1-49-60

Cited by 2 publications

(3 citation statements)

References 34 publications

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“…In our study, cn 1 demonstrated normal 2D STM in CCSP up to day 21 of adult life, but both KP mutants had impaired 8D LTM. The lack of 8D LTM was also shown for 5-day-old v 1 with absence of kynurenines and intact 3-h STM [ 42 ].…”

Section: Discussionmentioning

confidence: 95%

“…Our data also suggest different mechanisms for the Drosophila STM and LTM. In young flies, 8D LTM was absent in both KP mutants and v 1 , whereas 2D LTM was intact in v 1 and cn 1 , and 3-h STM was intact in all the studied strains [ 27 , 42 ]. LTM preservation up to day 8 seems to require robust memory mechanisms impaired to varying degrees in KP mutants.…”

Section: Discussionmentioning

confidence: 99%

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cd1 Mutation in Drosophila Affects Phenoxazinone Synthase Catalytic Site and Impairs Long-Term Memory

Zhuravlev

Ivanova

Makaveeva

et al. 2022

IJMS

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Being involved in development of Huntington’s, Parkinson’s and Alzheimer’s diseases, kynurenine pathway (KP) of tryptophan metabolism plays a significant role in modulation of neuropathology. Accumulation of a prooxidant 3-hydroxykynurenine (3-HOK) leads to oxidative stress and neuronal cell apoptosis. Drosophila mutant cardinal (cd1) with 3-HOK excess shows age-dependent neurodegeneration and short-term memory impairments, thereby presenting a model for senile dementia. Although cd gene for phenoxazinone synthase (PHS) catalyzing 3-HOK dimerization has been presumed to harbor the cd1 mutation, its molecular nature remained obscure. Using next generation sequencing, we have shown that the cd gene in cd1 carries a long deletion leading to PHS active site destruction. Contrary to the wild type Canton-S (CS), cd1 males showed defective long-term memory (LTM) in conditioned courtship suppression paradigm (CCSP) at days 5–29 after eclosion. The number of dopaminergic neurons (DAN) regulating fly locomotor activity showed an age-dependent tendency to decrease in cd1 relative to CS. Thus, in accordance with the concept “from the gene to behavior” proclaimed by S. Benzer, we have shown that the aberrant PHS sequence in cd1 provokes drastic LTM impairments and DAN alterations.

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Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 99%

cd1 Mutation in Drosophila Affects Phenoxazinone Synthase Catalytic Site and Impairs Long-Term Memory

Zhuravlev

Ivanova

Makaveeva

et al. 2022

IJMS

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“…In the first case, the control UAS > limk1"+" hybrid had bright eyes due to v[1] recessive allele, in contrast to UAS > limk1-KD hybrid with the wild type dark red eyes. The observed 3 h STM differences are unlikely to be associated with the differences in eyes pigmentation, as v[1] flies showed a normal 3 h STM and 2 day LTM in CCSP (Nikitina et al, 2021), while both forms of memory were impaired in the control strain. However, memory retention depends on parent affect, with some paternal epigenetic factors affecting STM strength (Medvedeva et al, 2021).…”

Section: генетика насекомых / Insect Geneticsmentioning

confidence: 99%

LIM-kinase 1 effects on memory abilities and male courtship song in Drosophila depend on the neuronal type

Zhuravlev,

Zalomaeva,

Egozova

et al. 2023

Vestn. VOGiS

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The signal pathway of actin remodeling, including LIM-kinase 1 (LIMK1) and its substrate cofilin, regulates multiple processes in neurons of vertebrates and invertebrates. Drosophila melanogaster is widely used as a model object for studying mechanisms of memory formation, storage, retrieval and forgetting. Previously, active forgetting in Drosophila was investigated in the standard Pavlovian olfactory conditioning paradigm. The role of specific dopaminergic neurons (DAN) and components of the actin remodeling pathway in different forms of forgetting was shown. In our research, we investigated the role of LIMK1 in Drosophila memory and forgetting in the conditioned courtship suppression paradigm (CCSP). In the Drosophila brain, LIMK1 and p-cofilin levels appeared to be low in specific neuropil structures, including the mushroom body (MB) lobes and the central complex. At the same time, LIMK1 was observed in cell bodies, such as DAN clusters regulating memory formation in CCSP. We applied GAL4 × UAS binary system to induce limk1 RNA interference in different types of neurons. The hybrid strain with limk1 interference in MB lobes and glia showed an increase in 3-h short-term memory (STM), without significant effects on long-term memory. limk1 interference in cholinergic neurons (CHN) impaired STM, while its interference in DAN and serotoninergic neurons (SRN) also dramatically impaired the flies’ learning ability. By contrast, limk1 interference in fruitless neurons (FRN) resulted in increased 15–60 min STM, indicating a possible LIMK1 role in active forgetting. Males with limk1 interference in CHN and FRN also showed the opposite trends of courtship song parameters changes. Thus, LIMK1 effects on the Drosophila male memory and courtship song appeared to depend on the neuronal type or brain structure.

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Effect of impaired kynurenine synthesis on memory in Drosophila

Cited by 2 publications

References 34 publications

cd1 Mutation in Drosophila Affects Phenoxazinone Synthase Catalytic Site and Impairs Long-Term Memory

cd1 Mutation in Drosophila Affects Phenoxazinone Synthase Catalytic Site and Impairs Long-Term Memory

LIM-kinase 1 effects on memory abilities and male courtship song in Drosophila depend on the neuronal type

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