Effect of Immunosuppression on Rejection of Cartilage Formed by Transplanted Allogeneic Rib Chondrocytes in Mice

Malejczyk, Jacek; Osiecka, Anna; Hyc, Anna; Moskalewski, Stanisław

doi:10.1097/00003086-199108000-00037

Cited by 20 publications

(12 citation statements)

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“…Allogeneic chondrocytes implanted into posterior tibial muscles formed nodules that immediately attracted macrophages, and natural killer and cytotoxic/suppressor T cells were also recruited to destroy the nascent cartilage over time [763]. This slow destruction of the repair tissue has been shown in several other studies [773][774][775][776]. Cells embedded in matrix, however, were relatively safe.…”

Section: Allogeneic Transplantsmentioning

confidence: 74%

Articular Cartilage Tissue Engineering

Athanasiou¹,

Darling²,

Hu³

2009

Synthesis Lectures on Tissue Engineering

184

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Section: Allogeneic Transplantsmentioning

confidence: 74%

Articular Cartilage Tissue Engineering

Athanasiou¹,

Darling²,

Hu³

2009

Synthesis Lectures on Tissue Engineering

184

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“…For example, chondrocytes and myoblasts do not activate alloreactive T cells in vitro [28,29] but these cell types are rapidly rejected after transplantation to immunocompetent recipients [30,31]. Furthermore, our own studies showed that MSCs primed alloreactive T cells and they had a partial stimulatory effect on unfractionated PBMCs in vitro.…”

Section: Discussionmentioning

confidence: 99%

T cell responses to allogeneic human mesenchymal stem cells: immunogenicity, tolerance, and suppression

et al. 2005

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Human mesenchymal stem cells (MSCs) were evaluated for their ability to activate allogeneic T cells in cell mixing experiments. Phenotypic characterization of MSCs by flow cytometry showed expression of MHC Class I alloantigens, but minimal expression of Class II alloantigens and costimulatory molecules, including CD80 (B7-1), CD86 (B7-2), and CD40. T cells purified from peripheral blood mononuclear cells (PBMCs) did not proliferate to allogeneic MSCs. Lack of response was not due to a deficiency of costimulation, since retroviral transduction of MSCs with either B7-1 or B7-2 costimulatory molecules did not result in lymphoproliferation. Although these results suggested that MSCs were immunologically inert or potentially tolerogenic, T cells cultured with MSCs produced IFN-gamma and displayed secondary kinetics to restimulation with PBMCs, indicating alloantigen priming rather than tolerance induction by the MSCs. To determine whether MSCs suppressed alloreactive T cells, MSCs were added to primary mixed lymphocyte reaction (MLR) cultures. MSCs suppressed cell proliferation when added at the initiation of culture or when added to an ongoing MLR culture. Suppression was dose-dependent, genetically unrestricted, and occurred whether or not MSCs were pretreated with IFN-gamma. MSCs in transwell chambers suppressed primary MLR cultures, indicating that suppression was mediated by soluble molecules. Analysis of cytokines in suppressed MLR cultures demonstrated up-regulation of IFN-gamma and IL-10, and down-regulation of TNF-alpha production relative to control cultures. We conclude that MSCs can initiate activation of alloreactive T cells, but do not elicit T cell proliferative responses due to active suppressive mechanisms.

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“…However, the immunoprivileged nature of cartilage has been challenged by other findings showing that both chondrocytes and their embedded ECM contain antigens and elicit varying degrees of immune reactions [34,38,[43][44][45]. Chondrocytes are liable to attack by natural killer cells [43,46] and also express major histocompatibility class (MHC) II antigens to trigger CD4 T lymphocytes and provoke cell or antibody-mediated immune responses [47][48][49]. Various degrees of immune response were reported after implantation of allogeneic chondrocytes grown on engineered scaffolds in osteochondral defects [50,51].…”

Section: Cartilage Immunogenicity and Necessity Of Decellularizationmentioning

confidence: 99%

Functionality of decellularized matrix in cartilage regeneration: A comparison of tissue versus cell sources

Sun

Yan²,

Chen

et al. 2018

Acta Biomaterialia

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The use of decellularized extracellular matrix (dECM) is becoming a promising approach for tissue engineering and regeneration. Compared to dECM derived from cartilage tissue, recently reported dECM from cell sources exhibits a distinct role in cell based cartilage regeneration. In this review paper, for the first time, tissue and cell based dECMs are comprehensively compared for their functionality in cartilage regeneration. This information is expected to provide an update for dECM based cartilage regeneration.

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Effect of Immunosuppression on Rejection of Cartilage Formed by Transplanted Allogeneic Rib Chondrocytes in Mice

Cited by 20 publications

References 0 publications

Articular Cartilage Tissue Engineering

Articular Cartilage Tissue Engineering

T cell responses to allogeneic human mesenchymal stem cells: immunogenicity, tolerance, and suppression

Functionality of decellularized matrix in cartilage regeneration: A comparison of tissue versus cell sources

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