Effect of immobilization on plant cell physiology - Real or imaginary?

Dainty, A.L.; Goulding, K. H.; Robinson, Peter; Simpkins, I.; Trevan, M. D.

doi:10.1016/0167-7799(85)90076-9

Cited by 14 publications

(4 citation statements)

References 10 publications

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“…Such cultures present characteristic data (Table 196) as well as showing incomplete utilization of carbon, reduced production of cell wall material and increased aeration needs. The latter indicates that respiration is increased relative to corresponding suspension cultures (Dainty et al 1985;Schnabel and Youngman 1985). …”

Section: Biological Aspectsmentioning

confidence: 97%

Plant Cell Biotechnology

Endreß

1994

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Section: Biological Aspectsmentioning

confidence: 97%

Plant Cell Biotechnology

Endreß

1994

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“…Despite the advantages of immobilization, the scientific and practical value of the strategy remains controversial (Dainty et al 1985). From a practical point of view, the present methods of plant cell immobilization are limited in their scale-up capabilities.…”

Section: Introductionmentioning

confidence: 99%

Immobilization of cultured Catharanthus roseus cells using a fibreglass substratum

Facchini

DiCosmo

1990

Appl Microbiol Biotechnol

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Cultured C a t h a r a n t h u s roseus cells were immobilized using geometrically identical needled fibreglass mats prepared with a range of surface coatings. The phenyl (PS), polyglycol (PG), aldehyde (CHO), alkyl (CTMS), and silanol (AW) coatings, along with the untreated glass (HC) surface, produced surfaces with a range of surface tensions. The immobilization efficiency of the substratum, measured as the percentage of cells immobilized, increased with increasing substratum surface tension in the order PS < PG < CHO < CTMS < AW < HC. The dependence of immobilization efficiency on substratum surface tension can be described using a thermodynamic model of adhesion that considers the extent of plant cell adhesion to be a function of the surface tensions of the substratum, the suspending liquid, and the plant cells. In addition, this dependence also demonstrates the fundamental role of adhesion in the immobilization process involving a glass fibre matrix. However, cell entrapment processes are also implicated. The untreated glass fibre substratum (HC), which demonstrated the greatest immobilization efficiency, was used for further characterization of the immobilization strategy. Maximum inoculum biomass was determined to be approximately 1.9 g cells (fresh weight)/g substratum (dry weight) to achieve greater than 90% immobilization efficiency. The growth rate of immobilized cultures was slower than suspension cultures, probably due to mass transfer limitations. Production of the indole alkaloids, tryptamine, catharanthine, and ajmalicine, was also suppressed relative to suspension-cultured cells. These results are considered in relation to other immobilization strategies and their apparent effects on cellular processes.

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“…However, immobilized plant cell systems, with cultures of J. officinale, J. odoratissimum or J. stephanese, using polyurethane from blocks, appear to be similar in many respects to callus tissue or cell aggregates without support matrices (Dainty et al 1985). Recently (I'Express 1986 April 25th, May 1st, Marchal-Rigaud 1986), the Japanese firm Kanebo, which in 1984 used cell suspensions from geranium leaves for their essential oil, has now started a programme (Fig.…”

Section: Cell Suspensionsmentioning

confidence: 99%

Jasminum spp. (Jasmine): Micropropagation and the Production of Essential Oils

Jonard

1989

Medicinal and Aromatic Plants II

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Effect of immobilization on plant cell physiology - Real or imaginary?

Cited by 14 publications

References 10 publications

Plant Cell Biotechnology

Plant Cell Biotechnology

Immobilization of cultured Catharanthus roseus cells using a fibreglass substratum

Jasminum spp. (Jasmine): Micropropagation and the Production of Essential Oils

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