Jasminum spp. (Jasmine): Micropropagation and the Production of Essential Oils

Jonard, R.

doi:10.1007/978-3-642-73617-9_17

Cited by 2 publications

(1 citation statement)

References 10 publications

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“…Bhattacharya and Bhattacharyya (2010) set a procedure for in vitro proliferation of J. officinale by newly developed adventitious axillary buds via proper phytohormonal stimulation. In earlier in vitro culture studies made by Jonard (1989) and Bhattacharya and Bhattacharyya (1997) showed use of nodal explants.…”

Section: Introductionmentioning

confidence: 99%

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2020

IJAEB

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Jasminum polyanthum is an important ornamental plant belonging to the Oleaceae family. Both shoot tip and leaves were used as explant for the induction of shoots via indirect organogenesis. For callus induction, explants were excised from field grown mature plants and thereafter incubated on MS basal medium supplied by 1.0 mg/l BA supplemented with various concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) at 0.0, 1.0, 2.0, 4.0 and 6.0 mg/l. Maximum percentage of callus induction from both explants was procured by the application of 2,4-D at 2.0 mg/l. Significant release of callus induction was swelled from leaf explants when compared to shoot tips at different 2,4-D levels. For the proliferation stage, induced callus was transferred into MS supplemented with BA at 0.0, 1.0, 2.0 and 3.0 mg/l along with NAA at 0.0, 0.5 and 1.0 mg/l. The maximum percentage of shoot proliferation per culture was achieved from callus cultured in 1.0 mg/L BA along with 0.5 mg/l NAA. For rooting, microshoots were treated with either IBA or IAA at 0.0, 0.5, 1.0 and 2.0 mg/l. Maximum rooting formation (Number and length) was recorded by the application of IBA at 2.0 mg/l. For acclimatization, the complete plantlets grown in pots filled with peat moss + vermiculite was suitable for 91% plantlets to survive.

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Section: Introductionmentioning

confidence: 99%

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2020

IJAEB

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In Vitro Propagation of Jasminum officinale L.: A Woody Ornamental Vine Yielding Aromatic Oil from Flowers

Bhattacharya

Bhattacharyya

2009

Protocols for in Vitro Propagation of Ornamental Plants

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The growing demand for flower extracts in perfume trade can primarily be met by increasing flower production and multiplying planting material. The major commercial aromatic flower yielding plants including Jasminum officinale L., a member of the Family Oleaceae have drawn the attention of a large section of the concerned sectors leading to a thrust upon developing advanced propagation technologies for these floral crops, in addition to conventional nature-dependent agro-techniques. This chapter describes concisely and critically, a protocol developed for in vitro propagation of Jasminum officinale by shoot regeneration from existing as well as newly developed adventitious axillary buds via proper phytohormonal stimulation. To start with nodal segments as explants, March-April is the most ideal time of the year when planting material suitable for in vitro multiplication is abundantly available. Prior to inoculation of explants in the culture medium, special care is needed to reduce microbial contamination by spraying on selected spots of the donor plant with anti-microbial agents 24 h prior to collection; treatment with antiseptic solution after final cleaning and surface sterilization by treating explants with mercuric chloride. Inoculated explants are free from brown leaching from cut ends by two consecutive subcultures within 48 h in MS basal medium. Multiplication of shoots, average 4-5 at each node, takes place in MS medium containing 4.0 mg/L BAP, 0.1 mg/L NAA, and 40 g/L sucrose over a period of 8 weeks. For elongation of regenerated shoots, cultures are transferred to MS medium, supplemented with a single growth hormone, kinetin at 2.0 mg/L. Emergence and elongation of roots from shoot base is facilitated by placing on the notch of a filter paper bridge. The hardened in vitro propagated plants are able to grow normally in soil like other conventionally propagated Jasminum officinale.

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Jasminum spp. (Jasmine): Micropropagation and the Production of Essential Oils

Cited by 2 publications

References 10 publications

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In Vitro Propagation of Jasminum officinale L.: A Woody Ornamental Vine Yielding Aromatic Oil from Flowers

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