“…The teeth were stored in an incubator (at 37 8C, 100% relative humidity). 35,36 After obturation, each specimen was mounted using autopolymerizing acrylic resin (Triplex; Ivoclar Vivadent, Schaan, Liechtenstein) in a trapezoidal mould perpendicular to the long access of the root with the use of a surveyor (Serveyor Parallometer System, Despley Ceramco, York, USA), to ensure that the path of the embedded canines was perpendicular to the CEJ. To simulate the periodontal ligament, the root surface of each tooth was coated with a layer of polyvinilsiloxane impression material (Virtual; Ivoclar Vivadent, Schaan, Liechtenstein) prior to embedding.…”