Effect of IGL-1, a new preservation solution, on kidney grafts (a pre-clinical study)

Abstract

Ischemia-reperfusion injury conditions short-term and long-term graft function. The effects of the inversion of K+ and Na+ concentrations and substitution with polyethylene glycol for hydroxyethyl starch in University of Wisconsin (K-UW) solution were evaluated in isolated perfused rat kidneys and in autotransplanted pig kidneys. In the rat model kidneys were cold-stored for 24 h in K-UW or Na-UW or Na-PEG UW solutions (IGL-1 solution). Fractional sodium reabsorption and glomerular filtration rate was better in kidneys preserved in Na-UW and IGL-1 solution than those preserved in K-UW solution. In the pig model the left kidney was harvested and preserved in K-UW or IGL-1 solution for 24 h and then transplanted. In the autotransplanted pig model, kidneys preserved in IGL-1 solution showed a better function and a significant reduction of MHC class II expression, cellular apoptosis and interstitial fibrosis. In conclusion, kidneys preserved in IGL-1 solution tolerated ischemia/reperfusion injury better than those preserved in K-UW solution.

Comparative Impact on Islet Isolation and Transplant Outcome of the Preservation Solutions Institut Georges Lopez-1, University of Wisconsin, and Celsior

“…IGL-1 has been tested for kidney preservation, showing that it performed equally to UW and could be superior for ischemically damaged kidneys (14,15). Similar results were reported for liver preservation (16 -18).…”

Evaluation of Institut Georges Lopez-1 Preservation Solution in Pig Pancreas Transplantation

“…The use of IGL-1 may reduce these adverse effects. IGL-1 has a low potassium concentration, which can prevent vasospasm (10,22,37); instead of HES, IGL-1 contains PEG35, which does not cause erythrocyte aggregation. PEG can also reduce platelet adhesion and shaping a clot with reduced contractility (41).…”

“…The emerging IGL-1 solution has shown beneficial outcomes in the preservation of kidney (17,22) and liver (23,24,26,27) and may represent an advance in optimizing pancreatic graft preservation. To the best of our knowledge, the described protocol, which includes 16-hr cold ischemia in a porcine whole PT model that closely resembles clinical PT, is the first study to assess the effectiveness of IGL-1 solution in PT.…”

“…Several experimental studies have shown that IGL-1 provides a good quality of preservation of kidney (22), liver (15,23,24), and intestine (25). Recently, in the clinical context, IGL-1 has been used with satisfactory results for kidney (17) and liver (26,27) transplantation and has also provided adequate performance in human pancreas preservation before islet isolation (28).…”

Deterioration of Endothelial and Smooth Muscle Cell Function in DCD Kidneys After Static Cold Storage in IGL-1 or UW

“…The first preservation solution is the University of Wisconsin (UW) solution, which is currently the most widely used kidney preservation solution [1]. The second preservation solution is Institut Georges Lopez-1 (IGL-1), which was developed by the Lyon Group in 1997 [13][14][15][16][17][18]. With regards to the present study, a few aspects of these preservation solutions are important.…”