Effect of IAA produced by Klebsiella oxytoca Rs-5 on cotton growth under salt stress

Liu, Yan; Shi, Zaiqiang; Yao, Lixia; Yue, Haitao; Li, Hui; Li, Chun

doi:10.2323/jgam.59.59

Cited by 43 publications

(18 citation statements)

References 29 publications

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“…SE992 had the capacity to produce IAA, which might compensate for the loss of endogenous IAA in plants affected by soil salinity. Similar findings were presented by Liu et al (2013), who found that IAA-producing Klebsiella oxytoca Rs-5 promoted cotton seedling growth under salt stress by enhancing endogenous IAA content. The significant increase in chlorophyll content in cucumber plants treated with Enterobacter sp.…”

Section: D 3dsupporting

confidence: 87%

Enterobacter sp. SE992-induced regulation of amino acids, sugars, and hormones in cucumber plants improves salt tolerance

et al. 2015

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The rhizosphere of plants serves as a host for a number of microbiota. The mutualistic interaction between rhizosphere bacteria and plants supports the promotion of plant growth. These interactions also support responses against biotic and abiotic stresses through the modulation of plant metabolism. In the current study, we sought to investigate the effects of individual sugar, amino acid, and salicylic acid content upon cucumber plant salt tolerance with respect to Enterobacter sp. SE992 interactions. Enterobacter sp. SE992 was isolated from soil and identified through 16s rDNA sequences. Mechanisms of plant growth promotion were confirmed via tryptophan-dependent indole-3-acetic acid synthesis in bacterial culture medium. Salt tolerance of Enterobacter sp. SE992 was confirmed on bacterial media supplemented with 3 % NaCl. Greenhouse experiments revealed that soil salinity inhibited cucumber plant growth. However, inoculation of Enterobacter sp. SE992 into saline soil mitigated the adverse effects of salt stress, enhancing the length and biomass of shoots and roots. The concentrations of chlorophyll, sucrose, glucose, and fructose were decreased in salt-affected plants. Interaction with Enterobacter sp. SE992 resulted in the enhanced production of chlorophyll and sugars in stressed plants. Salinity correlated with a significant decrease in threonine, glutamine, cysteine, valine, methionine, isoleucine, tyrosine, phenylalanine, histidine, and arginine levels but stimulated the accumulation of glycine, alanine, leucine, and lysine. Treatment with Enterobacter sp. SE992 ameliorated these stress effects and increased the levels of all amino acids. Marked accumulation of salicylic acid was observed in salt-infected plants, while inoculation with Enterobacter sp. SE992 suppressed salicylic acid synthesis. Our findings suggest that inoculation of Enterobacter sp. SE992 in soil can promote plant growth and increase stress tolerance by positively influencing plant metabolism under conditions of high salinity.

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Section: D 3dsupporting

confidence: 87%

Enterobacter sp. SE992-induced regulation of amino acids, sugars, and hormones in cucumber plants improves salt tolerance

et al. 2015

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“…For A. brasilense , a direct role for IAA production in the root architecture was demonstrated in wheat. In our study, EJ01 produced 43 mg/ml of IAA in liquid broth media, which was comparable with the amount produced by Klebsiella oxytoca Rs-5 (42.14 mg/ml broth), a bacterial strain that increases salt stress tolerance in cotton (Liu et al, 2013). This could be interpreted that auxin produced by EJ01 might be responsible for growth promotion and salt tolerance.…”

Section: Discussionsupporting

confidence: 74%

Alleviation of Salt Stress by Enterobacter sp. EJ01 in Tomato and Arabidopsis Is Accompanied by Up-Regulation of Conserved Salinity Responsive Factors in Plants

Kim¹,

Jang²,

Lee³

et al. 2014

Molecules and Cells

185

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Microbiota in the niches of the rhizosphere zones can affect plant growth and responses to environmental stress conditions via mutualistic interactions with host plants. Specifically, some beneficial bacteria, collectively referred to as Plant Growth Promoting Rhizobacteria (PGPRs), increase plant biomass and innate immunity potential. Here, we report that Enterobacter sp. EJ01, a bacterium isolated from sea china pink (Dianthus japonicus thunb) in reclaimed land of Gyehwa-do in Korea, improved the vegetative growth and alleviated salt stress in tomato and Arabidopsis. EJ01 was capable of producing 1-aminocy-clopropane-1-carboxylate (ACC) deaminase and also exhibited indole-3-acetic acid (IAA) production. The isolate EJ01 conferred increases in fresh weight, dry weight, and plant height of tomato and Arabidopsis under both normal and high salinity conditions. At the molecular level, short-term treatment with EJ01 increased the expression of salt stress responsive genes such as DREB2b, RD29A, RD29B, and RAB18 in Arabidopsis. The expression of proline biosynthetic genes (i.e. P5CS1 and P5CS2) and of genes related to priming processes (i.e. MPK3 and MPK6) were also up-regulated. In addition, reactive oxygen species scavenging activities were enhanced in tomatoes treated with EJ01 in stressed conditions. GFP-tagged EJ01 displayed colonization in the rhizosphere and endosphere in the roots of Arabidopsis. In conclusion, the newly isolated Enterobacter sp. EJ01 is a likely PGPR and alleviates salt stress in host plants through multiple mechanisms, including the rapid up-regulation of conserved plant salt stress responsive signaling pathways.

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“…On the other hand, a case for the involvement of ACC deamination as the main bacterial modulator of growth and tolerance to saline stress in plants is supported by examples involving AcdS mutants of Pseudomonas species (Cheng et al, 2012; Ali et al, 2014; Han et al, 2015) but, for the most part of the reports in literature, the actual extent of the contribution of this bacterial enzyme to salinity tolerance induction in the host is not explored in detail, and has been mostly inferred from the existence of a functional AcdS in active bacterial isolates (Onofre-Lemus et al, 2009; Ahmad et al, 2013; Chang et al, 2014; Singh et al, 2015). However, it is worth noting that, in several cases, high ACC deaminase activity levels in isolates do not correlate with a better performance in salinity tolerance induction (Zheng et al, 2008; Tank and Saraf, 2010; Tiwari et al, 2011; Liu et al, 2013; Mapelli et al, 2013; Ramadoss et al, 2013), or even growth promotion activity in general (Dey et al, 2004; Long et al, 2008; Bruto et al, 2014). Furthermore, it has been observed that, in order to be effective, ACC deamination within plant tissues must deal with feedback regulation of ethylene biosynthesis (Yang and Hoffman, 1984), that will stimulate ethylene production when ACC levels are low (Vacheron et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

“…Interestingly, it has been found that colonization of plants by certain specific plant growth promoting rhizobacteria (PGPR) can lead to enhanced resistance to abiotic challenges, such as water deficit (Naveed et al, 2014), salinity (Sziderics et al, 2007), adaptation to transplantation (Nowak and Shulaev, 2003), and chilling (Ait Barka et al, 2006). Over the last few years, several studies have reported the ability of isolated microorganisms to induce plant tolerance to salinity once they have been inoculated to seeds or young plantlets (reviewed in Yang et al, 2009; Dodd and Pérez-Alfocea, 2012; Shrivastava and Kumar, 2015), including a variety of hosts, like wheat (Nadeem et al, 2013; Singh et al, 2015), maize (Hamdia et al, 2004; Nadeem et al, 2009), cotton (Liu et al, 2013; Egamberdieva et al, 2015), tomato (Mayak et al, 2004; Ali et al, 2014), lettuce (Barassi et al, 2006; Kohler et al, 2009), sunflower (Shilev et al, 2010; Tewari and Arora, 2014) and Arabidopsis (Zhang et al, 2008; Kim et al, 2014; Sukweenadhi et al, 2015). Among the PGPR that have been demonstrated to play a role in salt stress tolerance induction, a wide diversity of bacteria is included, encompassing several members of the γ-proteobacteria class, specially within the genus Pseudomonas (Ahmad et al, 2013; Nadeem et al, 2013; Chang et al, 2014; Han et al, 2015), α-proteobacteria belonging to the Azospirillum genus (del Amor and Cuadra-Crespo, 2011; Nia et al, 2012; Sahoo et al, 2014), and β-proteobacteria like Achromobacter (Mayak et al, 2004) or Paraburkholderia (Talbi et al, 2013; Pinedo et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Volatile-Mediated Effects Predominate in Paraburkholderia phytofirmans Growth Promotion and Salt Stress Tolerance of Arabidopsis thaliana

et al. 2016

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Abiotic stress has a growing impact on plant growth and agricultural activity worldwide. Specific plant growth promoting rhizobacteria have been reported to stimulate growth and tolerance to abiotic stress in plants, and molecular mechanisms like phytohormone synthesis and 1-aminocyclopropane-1-carboxylate deamination are usual candidates proposed to mediate these bacterial effects. Paraburkholderia phytofirmans PsJN is able to promote growth of several plant hosts, and improve their tolerance to chilling, drought and salinity. This work investigated bacterial determinants involved in PsJN stimulation of growth and salinity tolerance in Arabidopsis thaliana, showing bacteria enable plants to survive long-term salinity treatment, accumulating less sodium within leaf tissues relative to non-inoculated controls. Inactivation of specific bacterial genes encoding ACC deaminase, auxin catabolism, N-acyl-homoserine-lactone production, and flagellin synthesis showed these functions have little influence on bacterial induction of salinity tolerance. Volatile organic compound emission from strain PsJN was shown to reproduce the effects of direct bacterial inoculation of roots, increasing plant growth rate and tolerance to salinity evaluated both in vitro and in soil. Furthermore, early exposure to VOCs from P. phytofirmans was sufficient to stimulate long-term effects observed in Arabidopsis growth in the presence and absence of salinity. Organic compounds were analyzed in the headspace of PsJN cultures, showing production of 2-undecanone, 7-hexanol, 3-methylbutanol and dimethyl disulfide. Exposure of A. thaliana to different quantities of these molecules showed that they are able to influence growth in a wide range of added amounts. Exposure to a blend of the first three compounds was found to mimic the effects of PsJN on both general growth promotion and salinity tolerance. To our knowledge, this is the first report on volatile compound-mediated induction of plant abiotic stress tolerance by a Paraburkholderia species.

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Effect of IAA produced by Klebsiella oxytoca Rs-5 on cotton growth under salt stress

Cited by 43 publications

References 29 publications

Enterobacter sp. SE992-induced regulation of amino acids, sugars, and hormones in cucumber plants improves salt tolerance

Enterobacter sp. SE992-induced regulation of amino acids, sugars, and hormones in cucumber plants improves salt tolerance

Alleviation of Salt Stress by Enterobacter sp. EJ01 in Tomato and Arabidopsis Is Accompanied by Up-Regulation of Conserved Salinity Responsive Factors in Plants

Volatile-Mediated Effects Predominate in Paraburkholderia phytofirmans Growth Promotion and Salt Stress Tolerance of Arabidopsis thaliana

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