Effect of Terminalia catappa Linn. on Biofilms of Candida albicans and Candida glabrata and on Changes in Color and Roughness of Acrylic Resin

Abstract

This study aimed to investigate the effect of the n-butanol fraction of Terminalia catappa Linn., (FBuTC) on biofilm of Candida albicans and Candida glabrata, as well as changes in color and roughness of polymethyl methacrylate resin (PMMA). The susceptibility of C. albicans and C. glabrata to FBuTC was evaluated by means of the Minimum Inhibitory and Minimum Fungicidal Concentration (MIC and MFC). PMMA acrylic resin discs (N= 108) were fabricated. For the susceptibility tests, biofilms of C. albicans and C. glabrata were developed on discs for 48 h and immersed in phosphate-saline buffer solution (PBS), 1% sodium hypochlorite (SH 1%), or FBuTC at MIC, 5xMIC, or 10xMIC. For the color and roughness change tests, the discs were immersed in distilled water, SH 1%, or FBuTC in the concentrations of 0.25 mg/mL, 2.5 mg/mL, or 25.0 mg/mL. After 28 days of incubation, color change was evaluated by spectrophotometry and roughness, by using a profilometer. The biofilms were investigated by one-way ANOVA and, the color and roughness changes (two-way ANOVA and the Tukey test; α=0.05). For both MIC and MFC the value of 0.25 mg/mL of FBuTC was observed for the planktonic cells of C. albicans and C. glabrata. Exposure to FBuTC at 10xMIC had a significant effect on the biofilm of C. albicans, showing a reduction in cell counts when compared with PBS, (p=0.001). For the biofilm of C. glabrata, the MIC was sufficient for significantly reducing the cell count (p<0.001). No important changes in color and roughness of the acrylic resin were observed, even after 28 days, irrespective of the concentration of FBuTC used (p >0.05). It could be concluded that the immersion of acrylic resin for dental prosthesis in FBuTC was effective in reducing the biofilms of C. albicans and C. glabrata without evidence of change in roughness and color of this substrate.

The effect of dental cleanser from plant extracts inhibiting the growth of Candida albicans on acrylic resin plates: a systematic review

“…7,8 Secondary metabolites of the phenolic group are obtained from mangrove leaves with active compounds in the form of flavonoid and saponin, 9 rosella flower with active compounds in the form of anthocyanin, riboflavin, beta-carotene, polysaccharide, and flavonoid, 10 ketapang leaves with active compounds in the form of tannins and n-butanol (Terminalia catappa Linn). Fraction, 11 Mexican tea extract with the active compound form quercetin and kaempferol, grape seed extract with active compounds in the form of polyphenols. 12 The terpenoid compounds are lipophilic; the antifungal activity in the secondary metabolites of the terpenoid group can cause the membrane to be blocked from dissolving lipids found on the cell membrane.…”

Ação do extrato de Arrabidaea chica em biofilmes de Candida albicans, efeito citotóxico em fibroblastos e queratinócitos, e nas propriedades físico/mecânicas de resina de poli(metilmetacrilato)

“…Neste sentido, pesquisas envolvendo compostos com propriedades antifúngicas de origem natural vêm aumentando (Gonçalves et al, 2019), inclusive na Odontologia. Desta forma, neste estudo, foi investigado e descrito pela primeira vez na literatura o efeito do extrato de A. chica em biofilmes de C. albicans sobre superfícies de resina acrílica, simulando a condição de biofilme na superfície de próteses dentais removíveis.…”

“…100 µL da solução stock do AchE foi transferida ao primeiro poço da placa de 96 poços e foi realizada a sua diluição seriada de forma a se obter o AchE nas concentrações 1,000 a 0.48 µg/ml. Nistatina (Sigma-Aldrich, St. Louis, MO, USA), Anfoterinina B (União Química Farmacêutica Nacional, SP, Brasil), e DMSO 2 % (veículo, v/v) (Gonçalves et al, 2019) foram utilizados como controles positivos e negativo, respectivamente. O meio de cultura estéril, o AchE, e a viabilidade do fungo também foram testadas.…”

Plants of the Genus Terminalia: An Insight on Its Biological Potentials, Pre-Clinical and Clinical Studies

“…Wight & Arn. ( Saivaraj and Chandramohan, 2018 ) and Terminalia catappa L. ( Gonçalves et al., 2019 ) bark, and Terminalia chebula Retz. fruit ( Vidya et al., 2019 ) extract demonstrated antifungal activity against the opportunistic yeasts including Candida albicans , C. glabrata , C. krusei , and C. tropicalis .…”

“…and Terminalia mantaly H. Perrier bark extracts inhibited Fusarium ssp., plant pathogenic fungi, with low MIC values ranging from 0.025–0.050 mg/mL ( Kouassi et al., 2019 ). Moreover, the leaf extract from Terminalia catappa L. exhibited antibiofilm activity against C. albicans and C. glabrata ( Gonçalves et al., 2019 ). The information indicated that the extracts of Terminalia sp.…”