Effect of Papaver rhoeas extract on in vitro maturation and developmental competence of immature mouse oocytes

Abstract

It was concluded that natural extracts increase the IVD competence of oocytes. The improved effect on oocyte maturation was dependent on the addition of optimum concentrations of extract to the maturation medium.

Superovulation, in vitro fertilization (IVF) and in vitro development (IVD) protocols for inbred BALB/cJ mice in comparison with outbred NMRI mice

“…In this research, we evaluated the effect of G-interval delivery on superovulation rate in addition to the effects of different media on in vitro embryo production during IVF and IVD for BALB/cJ mice. These evaluations were undertaken with the use of a common protocol in previous research [33][34][35][36][37] using Naval Medical Research Institute (NMRI) mice, which is an outbred mouse stock [31]. The common protocol included a 48 h G-interval [11,12,34,35] and used T6 as a medium during the IVF and IVD processes [34,35].…”

Effects of intraperitoneal administration of Papaver rhoeas L. extract on mouse ovaries

“…Therefore, effect of the extract is dose dependent. Our previous study showed that the administration of defined concentration of P. rhoeas extract to maturation medium increased IVM rates and subsequent developmental competence (Golkar-Narenji et al 2010). In this research, P. rhoeas extract administered in vivo and immature oocytes were retrieved from treated ovaries and matured in vitro.…”

“…into 10 female mice daily for 12 days. After 12 days of treatment, ovaries were immediately transferred to MEMa medium supplemented with 5% fetal bovine serum (Golkar-Narenji et al 2010), and oocytes were released using 26 gauge needles. Immature oocytes were transferred to maturation medium and about 16 hours later the maturation rate was recorded using an inverted microscope (Nikon).…”

The effect of intra‐peritoneal administration of Papaver bracteatum Lindl. extract on development of NMRI mice oocytes treated with Doxorubicin

“…It was found that oxidation is affected by antioxidants that can act as radical scavengers [71]. Investigations on the effect of P. rhoeas extract have revealed that extract of P. rhoeas can increase oocyte maturation and subsequent embryo development [15]. Recent studies have indicated the effects of antioxidants on the development of parthenogenetic porcine embryos.…”

“…The present study was designed to examine the possibility of an ameliorating action of the water-alcohol extract on doxorubicin-induced detrimental effects in mouse models, and implies that the extract may be a promising adjuvant agent that may attenuate the toxicity of doxorubicin. [14,15].…”