Effect of <i>Opuntia ficus indica</i> extract on methotrexate-induced testicular injury: a biochemical, docking and histological study

Akacha, Amira; Badraoui, Riadh; Rebaï, Tarek; Zourgui, Lazhar

doi:10.1080/07391102.2020.1856187

Cited by 51 publications

(74 citation statements)

References 70 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In our experiment, the treatment with ASO and ASE significantly decreased wound tissue levels TBARS, CD, AOPP and CP as compared to control rats. These results are in accordance with previous results [ 11 , 12 , 13 , 14 , 17 , 39 ], which reported that plants, specifically hairy garlic, possessed high antioxidant and free radical-scavenging effects. It may be attributed to the presence of active biomolecules in Allium subhirsutum L. oil which ultimately cause an antioxidant activity and wound repairing.…”

Section: Resultssupporting

confidence: 93%

See 1 more Smart Citation

The Role of Allium subhirsutum L. in the Attenuation of Dermal Wounds by Modulating Oxidative Stress and Inflammation in Wistar Albino Rats

et al. 2021

Self Cite

View full text Add to dashboard Cite

In our study, Allium subhirsutum L. (AS) was investigated to assess its phenolic profile and bioactive molecules including flavonoids and organosulfur compounds. The antioxidant potential of AS and wound healing activity were addressed using skin wound healing and oxidative stress and inflammation marker estimation in rat models. Phytochemical and antiradical activities of AS extract (ASE) and oil (ASO) were studied. The rats were randomly assigned to four groups: group I served as a control and was treated with simple ointment base, group II was treated with ASE ointment, group III was treated with ASO ointment and group IV (reference group; Ref) was treated with a reference drug “Cytolcentella® cream”. Phytochemical screening showed that total phenols (215 ± 3.5 mg GAE/g) and flavonoids (172.4 ± 3.1 mg QE/g) were higher in the ASO than the ASE group. The results of the antioxidant properties showed that ASO exhibited the highest DPPH free radical scavenging potential (IC50 = 0.136 ± 0.07 mg/mL), FRAP test (IC50 = 0.013 ± 0.006 mg/mL), ABTS test (IC50 = 0.52 ± 0.03 mg/mL) and total antioxidant capacity (IC50 = 0.34 ± 0.06 mg/mL). In the wound healing study, topical application of ASO performed the fastest wound-repairing process estimated by a chromatic study, percentage wound closure, fibrinogen level and oxidative damage status, as compared to ASE, the Cytolcentella reference drug and the untreated rats. The use of AS extract and oil were also associated with the attenuation of oxidative stress damage in the wound-healing treated rats. Overall, the results provided that AS, particularly ASO, has a potential medicinal value to act as effective skin wound healing agent.

show abstract

Section: Resultssupporting

confidence: 93%

“…Many antioxidants in medicinal plant extracts are used to eliminate the negative effects of ROS-associated pathologies and/or injuries including wound healing [ 11 , 13 , 14 ]. Over the years, medicinal plants have been used to develop a variety of formulations that combat injuries such as wounds, burns and cuts [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

The Role of Allium subhirsutum L. in the Attenuation of Dermal Wounds by Modulating Oxidative Stress and Inflammation in Wistar Albino Rats

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

“…The docking approach was based on the CHARMm force field after processing the receptors: polar hydrogens and Kollman charges. The binding affinity and the hydrogen‐bonds calculations were assessed as previously reported [37,38] . These receptors have been selected because they are highly implicated in the pathogenesis of breast and liver cancer [31,39] and microbial function, particularly S. aureus ligase [40] …”

Section: Methodsmentioning

confidence: 99%

Synthesis, Structure‐Activity Relationship and in silico Studies of Novel Pyrazolothiazole and Thiazolopyridine Derivatives as Prospective Antimicrobial and Anticancer Agents

et al. 2021

Self Cite

View full text Add to dashboard Cite

In our attempt to develop potential new drug candidates with promising dual antimicrobial and anticancer activities, a series of pyrazolothiazole and thiazolopyridine analogues has been synthesized. All compounds were evaluated for their antimicrobial potential towards pathogenic strains and cytotoxicity properties against hepatic cancer cell line HepG-2 and breast cancer cell line MCF-7. 9 c showed excellent antimicrobial activity against the tested strains, with MIC values about 27.5 μM (S. epidermidis), 6.8 (B. subtilis) and 3.4 μM (S. pneumoniae and K. pneumoniae) fold higher than the reference drug, whilst 9 a, 9 b and 5 a exhibited also potent activity against selected strains. Moreover, compounds 9 c (IC50 = 10.89 μM and 15.60 μM) and 9 a (IC50 = 22.24 μM and 28.47 μM) showed promising anticancer activity for HepG2 and MCF-7 cell lines, respectively, when compared to the known anticancer drugs, 5-Fluorouracil (IC50 = 26.75 μM and IC50 = 32.75 μM). The data from structure-activity relationships analysis revealed the potency of pyrazolothiazole than thiazolopyridine derivatives in generating potential activity. Further, molecular docking studies performed on the more active antimicrobial and cytotoxic compound, 9 c to get insights for binding modes to the target enzymes (PDB ID: 1JIJ) for antimicrobial, and (PDB ID: 1DI8) and (PDB ID: 3TWJ) for anticancer revealed that they interacted with the same key amino acids with TyrRS for S. aureus, CDK2 and ROCK1, respectively.

show abstract

“…Furthermore, serum testosterone levels increased considerably. Their results also suggest that EEOFI improves testicular injury and has a potent stimulating effects on fertility [94]. The contradictory results opens the field of research to confirm the antioxidant action of Opuntia genus on fertility.…”

Section: Effects On Human Infertilitymentioning

confidence: 96%

Opuntia genus in Human Health: A Comprehensive Summary on Its Pharmacological, Therapeutic and Preventive Properties. Part 1

et al. 2022

View full text Add to dashboard Cite

Opuntia spp. is a diverse and widely distributed genus in Africa, Asia, Australia, and America. Mexico has the largest number of wild species; mainly O. streptacantha, O. hyptiacantha, O. albicarpa, O. megacantha and O. ficus-indica. The latter being the most cultivated and domesticated species. Throughout history, plants and their phytochemicals have played an important role in health care and Opuntia spp. has shown a high nutritional, medicinal, pharmaceutical, and economic impacts. Its main bioactive compounds include pigments (carotenoids, betalains and betacyanins), vitamins, flavonoids (isorhamnetin, kaempferol, quercetin) and phenolic compounds. Together, they favor the different plant parts and are considered phytochemically important and associated with control, progression and prevention of some chronic and infectious diseases. This first review (Part 1), compiles information from published research (in vitro, in vivo, and clinical studies) on its preventive effects against atherosclerotic cardiovascular diseases, diabetes and obesity, hepatoprotection, effects on human infertility and chemopreventive and/or antigenotoxic capacity. The aim is to provide scientific evidences of its beneficial properties and to encourage health professionals and researchers to expand studies on the pharmacological and therapeutic effects of Opuntia spp.

show abstract

Effect of Opuntia ficus indica extract on methotrexate-induced testicular injury: a biochemical, docking and histological study

Cited by 51 publications

References 70 publications

The Role of Allium subhirsutum L. in the Attenuation of Dermal Wounds by Modulating Oxidative Stress and Inflammation in Wistar Albino Rats

The Role of Allium subhirsutum L. in the Attenuation of Dermal Wounds by Modulating Oxidative Stress and Inflammation in Wistar Albino Rats

Synthesis, Structure‐Activity Relationship and in silico Studies of Novel Pyrazolothiazole and Thiazolopyridine Derivatives as Prospective Antimicrobial and Anticancer Agents

Opuntia genus in Human Health: A Comprehensive Summary on Its Pharmacological, Therapeutic and Preventive Properties. Part 1

Contact Info

Product

Resources

About