Effect of Mucuna pruriens (Linn.) on Sexual Behavior and Sperm Parameters in Streptozotocin-Induced Diabetic Male Rat

Abstract

Introduction Sexual dysfunction is one of the major secondary complications in the diabetic. Mucuna pruriens, a leguminous plant identified for its antidiabetic, aphrodisiac, and improving fertility properties, has been the choice of Indian traditional medicine. Aim Objective of the present study was to analyze the efficacy of M. pruriens on male sexual behavior and sperm parameters in long-term hyperglycemic male rats. Methods Male albino rats were divided as group I control, group II diabetes induced (streptozotocin [STZ] 60 mg/kg of body weight (b.w.) in 0.1 M citrate buffer), group III diabetic rats administered with 200 mg/kg b.w. of ethanolic extract of M. pruriens seed, group IV diabetic rats administered with 5 mg/kg b.w. of sildenafil citrate (SC), group V administered with 200 mg/kg b.w. of extract, and group VI administered with 5 mg/kg b.w. of SC. M. pruriens and SC were administered in single oral dosage per day for a period of 60 days. The animals were subjected to mating behavior analyses, libido, test of potency, and epididymal sperms were analyzed. Main Outcome Measure The mating behavior, libido, test of potency, along with epididymal sperms were studied. Results The study showed significant reduction in sexual behavior and sperm parameters in group II. Daily sperm production (DSP) and levels of follicular stimulating hormone, luteinizing hormone, and testosterone were significantly reduced in group II, whereas the animals with diabetes administered with seed extract of M. pruriens (group III) showed significant improvement in sexual behavior, libido and potency, sperm parameters, DSP, and hormonal levels when compared to group II. Conclusion The present work reveals the potential efficacy of ethanolic seed extract of M. pruriens to improve male sexual behavior with androgenic and antidiabetic effects in the STZ-induced diabetic male rats. This study supports the usage of M. pruriens in the Indian system of medicine as sexual invigorator in diabetic condition and encourages performing similar study in men.

Protective effects of <i>Lycium barbarum</i> polysaccharide on male sexual dysfunction and fertility impairments by activating hypothalamic pituitary gonadal axis in streptozotocin-induced type-1 diabetic male mice

“…The training was performed on mice for 30 min after 5 pm each day for 3 consecutive days. The male mice which failed to perform intromission within 5 min were excluded from the study as they were considered sexually inactive [33,34]. In this step, 6 out of 14 mice in diabetic group, 0 out of 10 mice in control group, 3 out of 13 mice in SC group, and 4 out of 12 mice, 5 out of 13 mice and 2 out of 14 mice in LBP (10, 20, 40 mg/kg) were excluded from the study respectively.…”

“…The right cauda epididymis was weighed and finely dissected and placed in 1 mL of prepared Ham's F10 medium for 30 min to allow the spermatozoa to swim into the culture medium freely. Sperm count (10 6 /mL) and motility was assessed using a Makler chamber [34,36]. …”

Melatonin Ratlarda Alloxan İle İndüklenen Diyabet Sonrası Oluşan Testiküler Hasarı ve Oksidatif Etkileri Hafifletir

“…It was reported in some studies that the rats having undergone the STZ had decreased sperm motility, but without any marked change [4,37,[40][41][42][43][44] . Similarly, herein, we did not find any difference neither between nor within the groups on the 15 th and 45 th d.…”

“…No marked change in total sperm number was observed by Abbasi et al [37] , while the daily sperm production in rats and the sperm concentration in mice and rats decreased in the STZ-induced diabetes [4,[40][41][42][43][44] . Herein, the sperm concentration in the 45 th d markedly decreased in the DM group as compared to those in the C group, but the MLT treatment of diabetic rats did not lead to recovering effect for a decrease in the sperm concentration.…”

“…Other antioxidants may not reverse the deterioration in these parameters, but they may restore the daily sperm production in testis, and alleviate the mitochondrial function and DNA damage [42] . Deterioration of spermatologic parameters due to the administration of various substances (with ROS generating activities within the testis), the MLT, used as an antioxidant, could ameliorate these parameters [60][61][62][63] .…”