Effect of<i>Moringa oleifera</i>seed extract on antioxidant activity and sperm characteristics in cryopreserved ram semen

Carrera‐Chávez, José Maria; Jiménez-Aguilar, Edson Eduardo; Acosta-Pérez, Theisy Patricia; Núñez‐Gastélum, José Alberto; Quezada‐Casasola, Andrés; Escárcega‐Ávila, Angélica María; Itzá-Ortíz, Mateo; Orozco‐Lucero, Ernesto

doi:10.1080/09712119.2020.1741374

Cited by 21 publications

(19 citation statements)

References 29 publications

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“…It was thought that these positive effects of TQ are due to its supporting influence on antioxidant defence (Mahdavi et al, 2015). In the freezing studies of ram semen with different plant extracts, including Moringa Oleifera (Carrera-Chavez et al, 2020), Entada abyssinica (Sobeh et al, 2020), Ginger and echinacea (Merati & Farshad, 2020) extracts, it has been determined that these extracts increase antioxidant activity after thawing and have curative effects on spermatological parameters. Accordingly, TQ also demonstrated superoxide anion scavenger effects, androgen activities and direct cryoprotective and indirect antioxidant activities (Hala, 2011).…”

Section: Discussionmentioning

confidence: 99%

Thymoquinone improves motility, plasma membrane integrity and DNA integrity of frozen–thawed ram semen

et al. 2022

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The aim of this study was to determine the effects of thymoquinone (TQ), which is the most essential active compound of Nigella sativa, on the spermatological parameters of ram semen during cryopreservation. Ejaculates were collected from five Sonmez rams using an artificial vagina and extended with Tris‐based extender not containing TQ (control, 0 μg/ml TQ) and containing 10, 25, 50 and 100 μg/ml TQ. The extended semen samples were equilibrated in a + 4°C cold cabinet for 2 h. After 2 h, the samples were loaded into 0.25 ml French straws. The straws were frozen by liquid nitrogen vapour and stored in a liquid nitrogen container (−196°C). The frozen straws were thawed in a water bath (37°C for 30 s) and evaluated in terms of motility characteristics, plasma membrane and acrosome integrity, mitochondrial reactive oxygen species levels, lipid peroxidation levels, DNA damage and biochemical alterations (oxidative stress index, malondialdehyde and glutathione). TQ100 had higher total motility (53.59 ± 3.01) and progressive motility (19.84 ± 1.44; not significantly different from TQ25 and TQ50) compared to the control and TQ10 (p ˂ 0.05). According to the results of the analyses on motility characteristics, there were significant differences between the groups in terms of curvilinear velocity (VCL), amplitude of lateral head displacement (ALH) and linearity (LIN; p ˂ 0.05). The highest DNA damage was detected in the control group (p ˂ 0.05). TQ50 had higher plasma membrane and acrosome integrity (59.56 ± 5.92) compared to the control and TQ25 (p < 0.05) but not significantly different from TQ10 and TQ100. The lowest mitochondrial reactive oxygen species levels were detected in TQ50 and TQ100 (p ˂ 0.05). There were no significant differences among the groups in terms of their oxidative stress index, lipid peroxidation, malondialdehyde and glutathione levels (p > 0.05). According to the results, it could be concluded that supplementing 50 or 100 μg/ml TQ to Tris extenders that were used for ram semen cryopreservation showed a positive effect on motility, plasma membrane integrity and acrosome integrity, and it reduced DNA damage and mitochondrial reactive oxygen species levels.

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Section: Discussionmentioning

confidence: 99%

Thymoquinone improves motility, plasma membrane integrity and DNA integrity of frozen–thawed ram semen

et al. 2022

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“…Adding plant-based extracts to freezing media improves the antioxidant properties of freezing media [ 121 ]. Including Moringa oleifera extract in freezing media lowered the harmful effects on frozen thawed sperm quality in sheep [ 122 ]. Similarly, ovine sperm acrosome integrity, motility and viability were improved by the addition of an aqueous solution of rosemary [ 123 ].…”

Section: Addition Of Seminal Plasma Components and Other Supplementsmentioning

confidence: 99%

Effect of Sperm Cryopreservation in Farm Animals Using Nanotechnology

Akhtar

et al. 2022

Animals

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Sperm cryopreservation is one of the sublime biotechnologies for assisted reproduction. In recent decades, there has been an increasing trend in the use of preserved semen. Post-thaw semen quality and values vary among animals of the same species. Similarly, there are species-specific variations in sperm morphology, i.e., sperm head, kinetic properties, plasma membrane integrity, and freezability. Similarly, the viability of sperm varies in the female reproductive tract, i.e., from a few hours (in cattle) to several days (in chicken). Various steps of sperm cryopreservation, i.e., male health examination, semen collection, dilution, semen centrifugation, pre- and post-thaw semen quality evaluation, lack standardized methodology, that result in differences in opinions. Assisted reproductive technologies (ART), including sperm preservation, are not applied to the same extent in commercial poultry species as in mammalian species for management and economic reasons. Sperm preservation requires a reduction in physiological metabolism by extending the viable duration of the gametes. Physiologically and morphologically, spermatozoa are unique in structure and function to deliver paternal DNA and activate oocytes after fertilization. Variations in semen and sperm composition account for better handling of semen, which can aid in improved fertility. This review aims to provide an update on sperm cryopreservation in farm animals.

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“…Higher post-thaw sperm motility and viability were reported following fortification of Barki ram freezing extender with MOLE (Shokry et al 2021). In rams, Moringa oleifera seed methanolic extract improved post-thaw semen motility and antioxidant capacity (Carrera-Chávez et al 2020). The biochemical analysis of chilled and frozen ram semen was reported (Abd El-Hamid 2019).…”

Section: Introductionmentioning

confidence: 98%

The impact of varying doses of moringa leaf methanolic extract supplementation in the cryopreservation media on sperm quality, oxidants, and antioxidant capacity of frozen-thawed ram sperm

El-Seadawy

Kotp

El‐Maaty

et al. 2022

Trop Anim Health Prod

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To increase rams’ post-thaw semen quality following cryopreservation, this study used enriched Tris-based diluent with varying amounts of moringa leaf methanolic extract (MLME). The antioxidant activity, total phenolic, and total flavonoid content were all assessed in MLME. The sperm of five healthy Awassi rams were collected, divided into 4 equal aliquots, and diluted [1:5; (v/v)] in Tris-citrate-glucose extender supplemented with 0.48, 0.56, and 0.64 mg MLME/ml or without MLME supplementation (control). The percentages of sperm total motility (STM, %), sperm progressive motility (SPM, %) and viability (V, %), abnormal morphology (AM, %), membrane functional integrity (MFI, %), and acrosome integrity (AI %) were measured. Malondialdehyde (MDA), nitric oxide (NO), ascorbic acid (AA), superoxide dismutase (SOD), glutathione peroxidase (GPx), total cholesterol (TC), low-density lipoproteins (LDL), lactate dehydrogenase (LDH), alkaline phosphatase (ALP), zinc (Zn), and copper (Cu) were measured. The total phenolic gallic acid and flavonoid catechin (equivalent) contents were 19.78 mg/g and 11.94 mg/g, respectively. 2,2-Diphenyl-1-picrylhydrazyl (34.37 mM TE/g) and 2,2′-azino-bis/3-ethylbenzothiazoline-6-sulfonic acid (53.47 mM TE/g) were found in MLME. MLME had a 64.59 mM TE/g ferric-reducing power. In comparison to control, the addition of 0.64 mg/ml MLME to Tris-based extender resulted in the highest (P < 0.001) STM (55.22 ± 0.98), SPM (45.41 ± .70), SV (60.01 ± 1.05), MFI (75.23 ± 0.77), and AI (73.13 ± 0.72) and the lowest (P < 0.001) AM (21.34 ± 0.72) values. In comparison to the control, the addition of 0.56 mg/ml semen extender resulted in lower STM, SPM, SV, MFI, and AI with higher AM percentages. MDA (P = 0.03), NO (P = 0.012), CHO (P = 0.0001), and LDL (P = 0.004) were reduced by 0.64 mg/ml MLME, while AA (P = 0.017) and SOD (P = 0.0001) were elevated. In conclusion, the highest copper (P = 0.006) and lowest zinc concentrations in MLME (0.48 mg/ml extender) deteriorated the post-thaw semen quality, prompting us to suggest the addition of 0.64 mg MLME to rams’ Tris-based semen extender.

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Effect ofMoringa oleiferaseed extract on antioxidant activity and sperm characteristics in cryopreserved ram semen

Cited by 21 publications

References 29 publications

Thymoquinone improves motility, plasma membrane integrity and DNA integrity of frozen–thawed ram semen

Thymoquinone improves motility, plasma membrane integrity and DNA integrity of frozen–thawed ram semen

Effect of Sperm Cryopreservation in Farm Animals Using Nanotechnology

The impact of varying doses of moringa leaf methanolic extract supplementation in the cryopreservation media on sperm quality, oxidants, and antioxidant capacity of frozen-thawed ram sperm

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