Effect of<i>Lactobacillus rhamnosus</i>CGMCC1.3724 supplementation on weight loss and maintenance in obese men and women

Sánchez, Marina; Darimont, Christian; Drapeau, Vicky; Emady-Azar, Shahram; Lepage, Mélissa; Rezzonico, Enea; Ngom-Bru, Catherine; Berger, Bernard; Philippe, Lionel; Ammon-Zuffrey, Corinne; Leone, Patricia; Chevrier, Geneviève; St-Amand, Emmanuelle; Marette, André; Doré, Jean; Tremblay, Angelo

doi:10.1017/s0007114513003875

Cited by 274 publications

(153 citation statements)

References 48 publications

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“…Similar weight loss was shown in obese humans who consumed purified L. rhamnosus [40]. It was also previously shown that microbe-induced oxytocin modulates host immunity by inducing a more rapid return to health after injury [30].…”

Section: Discussionsupporting

confidence: 75%

“…Based on our knowledge that consumption of Lactobacillus ATCC 6475 is sufficient to inhibit inflammation and age-associated obesity in mouse models [39], and Lactobacillus rhamnosus CGMCC1.3724 stimulates weight loss in obese humans [40], we tested the microbeobesity hypothesis using mice exposed orally to the canine-source L. reuteri 2546 and compared them with untreated control animals. After nine months of daily feeding with L. reuteri, we discovered that L. reuteri 2546-treated mice had significantly lower body weight than control animals ( Figure 2A).…”

Section: Mice Exposed To Bacteria From Dog Saliva Are More Slender Thmentioning

confidence: 99%

See 1 more Smart Citation

Beneficial Dog Bacteria Up-Regulate Oxytocin and Lower Risk of Obesity

Varian¹,

Levkovich²,

Poutahidis³

et al. 2017

J Prob Health

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AnimalsC57BL/6 wild type (wt), oxytocin-wt (oxt-wt) and oxytocin- AbstractCohabitation with pet dogs imparts diverse health benefits to humans including a slim physique. It is known that neuropeptide hormone oxytocin fundamental in human-canine social bonds regulates appetite and body weight. It was recently shown in mice that consuming Lactobacillus reuteri ATCC 6475 from human breast milk lowers body weight and up-regulates oxytocin levels in blood. Here we test the hypothesis that bacteria from dog saliva may similarly modulate recipient host body weight. We find that a Lactobacillus spp isolate from dog saliva led to lower body weight when fed to C57BL/6 wild type mice. Mice consuming the canineborne L. reuteri also had elevated oxytocin levels in blood plasma, and exhibited body weight in an oxytocin-dependent manner. Interestingly, killed (lysed) canine bacteria were sufficient to achieve the physiological effects. Taken together, these studies provide evidence that dog bacteria modulate oxytocin levels and body weight in recipient mice, and thus may help reduce risk of obesity in individuals cohabitating with pet dogs. knockout (oxt-ko) B6; 129S-Oxttm1Wsy/J mice (purchased initially from Jackson labs; Bar Harbor, ME) were used in three separate experiments (Figure 1). Mice were housed and handled in Association for Assessment and Accreditation of Laboratory Animal Care (AAALAC)-accredited facilities using techniques and diets including Lactobacillus reuteri as specifically approved by Massachusetts Institute of Technology's (MIT) Committee on Animal Care (CAC). Mice were housed under standard 12:12 light cycle conditions with lights on at 7 AM. Mice were fed a standard control chow Purina RMH3000. Beneficial Dog Bacteria Up-Regulate Oxytocin and Lower Risk of ObesityMice were bred in-house to achieve experimental groups. Each experiment included 5-15 animals per group with one or two replications (total N=10-30 mice examined per group) unless otherwise specified. For the initial studies, C57BL/6 wt mice received Lactobacillus reuteri 2546 isolated from dog saliva. To test putative roles for microbeinduced oxytocin in obesity, oxt-ko mice and their oxt-wt littermates entered experiments at eight weeks of age. At the conclusion of the study mice were euthanized with CO 2 overdose, and were examined as described below. Eight pet dogs served as saliva microbe donors as approved by the MIT-CAC. Saliva was collected from these dogs in the morning before feeding using sterile swabs [Puritan Sterile Polyester Tipped applicators Guilford, Me Ref: 25-806 1PD] in 1.5 ml centrifuge tubes (Safeseal Microcentrifuge Tubes, Sorenson Bioscience Inc. Salt Lace City, UT Cat# 16070). Bacteria were purified from dog saliva as described in detail below. PCR of dog saliva for all Lactobacillus speciesSaliva was collected from eight pet dogs and prepared using High Pure PCR Template kit (Roche Diagnostics) was used without changes to the manufacturer's directions to isolate DNA from the canine saliva. DNA was measured using Nan...

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Section: Discussionsupporting

confidence: 75%

Section: Mice Exposed To Bacteria From Dog Saliva Are More Slender Thmentioning

confidence: 99%

Beneficial Dog Bacteria Up-Regulate Oxytocin and Lower Risk of Obesity

Varian¹,

Levkovich²,

Poutahidis³

et al. 2017

J Prob Health

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“…Pyruvate oxidation was used to assess the activity of pyruvate dehydrogenase (PDH), the enzyme that catalyzes the oxidation of pyruvate and the provision of glucose-derived acetyl CoA to the TCA. [1][2][3][4][5][6][7][8][9][10][11][12][13][14] C]-pyruvate oxidation was assessed in a similar manner to fatty acid oxidation with the exception that pyruvate was substituted for BSA-bound palmitic acid. All samples were run in triplicate and data were normalized to total protein content and expressed in nmol/mg protein/h.…”

Section: Serum Endotoxin Was Measured With a Pyrogenementioning

confidence: 99%

“…In addition, small, but significant, body mass and fat mass loss has been reported in individuals with obesity following consumption of some probiotic strains (7,8). Interestingly, Hultson et al (9) reported less body mass and fat mass gain and prevention of insulin resistance in young, healthy subjects consuming a single probiotic strain during 7 days of high-fat overfeeding.…”

Section: Introductionmentioning

confidence: 99%

Probiotic supplementation attenuates increases in body mass and fat mass during high‐fat diet in healthy young adults

Osterberg

Boutagy

McMillan

et al. 2015

Obesity

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Objective: The objective was to determine the effects of the probiotic, VSL#3, on body and fat mass, insulin sensitivity, and skeletal muscle substrate oxidation following 4 weeks of a high-fat diet. Methods: Twenty non-obese males (18-30 years) participated in the study. Following a 2-week eucaloric control diet, participants underwent dual X-ray absorptiometry to determine body composition, an intravenous glucose tolerance test to determine insulin sensitivity, and a skeletal muscle biopsy for measurement of in vitro substrate oxidation. Subsequently, participants were randomized to receive either VSL#3 or placebo daily during 4 weeks of consuming a high-fat (55% fat), hypercaloric diet (11,000 kcal day 21). Participants repeated all measurements following the intervention. Results: Body mass (1.42 6 0.42 kg vs. 2.30 6 0.28 kg) and fat mass (0.63 6 0.09 kg vs. 1.29 6 0.27 kg) increased less following the high-fat diet in the VSL#3 group compared with placebo. However, there were no significant changes in insulin sensitivity or in vitro skeletal muscle pyruvate and fat oxidation with the high-fat diet or VSL#3. Conclusions: VSL#3 supplementation appears to have provided some protection from body mass gain and fat accumulation in healthy young men consuming a high-fat and high-energy diet.

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“…Again, the global profile of the faecal microbiota was not altered except for a transient increase in the two probiotic strains during supplementation. In adults, L. rhamnosus combined with a weight reduction diet had no overall impact on faecal microbiota composition and weight loss, while subgroup analysis showed an effect in women (Sanchez et al ., 2014). Overall, the trials showed a great resilience of the microbiota in adults towards probiotic supplementation.…”

Section: Introducing Beneficial Bacteria Into the Gut Microbiomementioning

confidence: 99%

Biome engineering‐2020

Brüssow

2016

Microbial Biotechnology

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SummaryThe gut microbiome research is going from a descriptive into an intervention phase. To optimize beneficial microbe–host interaction, we need to understand how to steer the system by modulating the nutrient input with which the system is literally fed (e.g. diets, fibres, prebiotics, human milk oligosaccharides), and we must learn how to modulate the composition of the gut microbiota by adding beneficial microbes (e.g. probiotics, faecal transplants) and by eliminating disturbing microbial members using, for example, bacteriophages in this highly complex ecosystem. The current status of the field is reviewed together with an outlook what might be expected until 2020, highlighting obstacles to progress and possible solutions to these problems.

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Effect ofLactobacillus rhamnosusCGMCC1.3724 supplementation on weight loss and maintenance in obese men and women

Cited by 274 publications

References 48 publications

Beneficial Dog Bacteria Up-Regulate Oxytocin and Lower Risk of Obesity

Beneficial Dog Bacteria Up-Regulate Oxytocin and Lower Risk of Obesity

Probiotic supplementation attenuates increases in body mass and fat mass during high‐fat diet in healthy young adults

Biome engineering‐2020

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