Effect of Lactobacillus acidophilus supplementation on growth performances, digestive enzyme activities and gut histomorphology of striped catfish (Pangasianodon hypophthalmus Sauvage, 1878) juveniles

Abstract

The influence of dietary supplementation of Lactobacillus acidophilus, on growth performance, digestive enzyme activities, gut histomorphology and gut microflora were evaluated in juveniles striped catfish (Pangasianodon hypophthalmus). Five experimental diets were formulated by supplementing 0, 10 3 , 10 5 , 10 7 and 10 9 CFU/g L. acidophilus in fishmeal and casein-based semi-purified diet. Triplicate groups of striped catfish (21.69 ± 0.18 g) were stocked in 15 fiberglass tanks with stocking density of 25 individuals per tank and fed twice daily at 2.5% of the fish body weight for 12 weeks. Weight gain, specific growth rate, feed conversion ratio and the protein efficiency ratio were significantly higher in fish fed with 10 5 and 10 7 CFU/g L. acidophilus supplemented diets compared with the other treatment groups. Compared with the control and fish fed low (10 3 CFU/g) L. acidophilus supplementation, those fed with 10 5 and 10 7 CFU/g had significantly higher (p < 0.05) apparent protein digestibility. Inclusion of L. acidophilus at 10 7 CFU/g diet significantly increased amylase, protease and lipase activities. Microscopic analysis showed that the villi length in both the anterior and posterior gut and microvilli length in the posterior gut increased significantly in fish fed L. acidophilus supplementation at 10 5 and 10 7 CFU/g of diet. The fish fed L. acidophilus supplemented diets significantly increased the total lactic acid bacteria counts in the gut of striped catfish compared with the control-fed group. Based on gut histomorphology and growth performance, inclusion of L. acidophilus at 10 5 CFU/g appears to have the most positive effect on striped catfish farming. K E Y W O R D Sdigestive enzyme activities, growth performance, gut morphology, Lactobacillus acidophilus, Pangasianodon hypophthalmus

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Low level incorporation of Lactobacillus Sporogenes in feed of Tinfoil Barb (Barbonymus Schwanenfeldii (Bleeker, 1854)) Fingerlings Positively Improves Growth, Gut Microflora and Intestinal Morphology

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