Low level incorporation of Lactobacillus Sporogenes in feed of Tinfoil Barb (Barbonymus Schwanenfeldii (Bleeker, 1854)) Fingerlings Positively Improves Growth, Gut Microflora and Intestinal Morphology

Abstract

Use of probiotics in freshwater fishes is debated for its benefits to the host. Tinfoil barb, Barbonymus schwanenfeldii is a tropical fish having ornamental as well as food value. This study was conducted to evaluate effect of Lactobacillus sporogenes on Tinfoil barb. Diets containing 0%(T0), 0.5%(T1), 1%(T2), 1.5%(T3), and 2% (T4) of probiotic L. sporogenes were prepared and fed to fishes at 3% body weight for 80 days. Control diet was fed to fishes of all treatments for 20 more days. Growth parameters, gut microflora and intestinal histology were analysed. The growth performance was found to be significantly better (P < 0.05) in fishes fed with 1% of probiotic but Survival remained unaffected due to the concentration of probiotic. Total Plate Count of aerobes seemed to be affected by concentration and duration of probiotic treatment. However, anaerobic TPC did not show much variation. Histological analysis revealed that in lower level of incorporation (T2-1%) fish have intact mucosal layer with increased absorptive enterocytes and absorptive area of intestinal villi, well organized lamina propria with different types of cells compared to other treatments. The results indicated that addition of L. sporogenes at 1% level of inclusion was found to be effective for better growth, survival, gut flora as well as intestinal morphology in B. schwanenfeldii.

Analysis of Embryo Development and Early Performance of Larvae of Barbonymus Schwanenfeldii: A Systematic Review

“…According to Wahyuningtias et al ( 2015) at a good environmental temperature embryonic development, egg hatching, and early survival of fish larvae can take place normally. At high temperatures it will accelerate metabolism, so that embryonic development will be faster, and can inhibit the hatching process or cause death in larvae 11 . A good temperature for hatchery of freshwater fish ranges from 25-31 C Furthermore, the fish eggs enter the morula phase.…”