Effect of Corynebacterium parvum on hemopoietic stem‐cell kinetics

Abstract

The intravenous administration of 548 microgram of killed Corynebacterium parvum (C. parvum) into C57BL mice leads to a significant decrease in the number of bone-marrow colony-forming-units in spleen (CFUs) as early as 12 h after the injection of the bacterium. This decrease persisted in varying degrees for 3 weeks. After an initial fall at 24 h, the splenic CFUs exhibited a rapid expansion and reached values 10 times higher than the control range on the ninth day. A significant rise in the number of circulating CFUs, reaching a first peak at 2 h and a second one on the fifth day, was also observed. The proliferative status of femoral CFUs was increased at 48 and 72 h, while that of splenic CFUs presented a significant increase only 48 h after the injection of C. parvum. The sequence of events which were observed in these experiments indicates that an accelerated migration of hemopoietic stem cells from bone marrow to spleen via the blood circulation has to take place.

A mouse model for immunotherapy of osteosarcoma

“…Three potential sites exist for the anti-turnout actions of PGEs. Systemic Corynebacterium parvum is thought to exert its non-specific anti-tumour effect by increasing the output of mononuclear phagocytes from the marrow [6,10,12,42] and/or by activating macrophages [3,17], and NK cells via interferon [1,2,40]. Systemic Corynebacterium parvum is thought to exert its non-specific anti-tumour effect by increasing the output of mononuclear phagocytes from the marrow [6,10,12,42] and/or by activating macrophages [3,17], and NK cells via interferon [1,2,40].…”

Effect of periodate treatment and phenol extraction of C. parvum on its capacity to activate macrophages

“…After SC injection the nearest lymph node becomes labelled and when C. parvum is injected intratumorally (IT) it remains there for as long as 15 days [29]. It has been found that soon after C. parvum injection, macrophages infiltrate the lymphoid organs and the liver [4,5] Data provided by Scott et al [29] demonstrated that the peritoneal and bone marrow cells are able to take up C. parvum quite readily when it is injected IP or IV. Moore et al [18] have shown that tumours are invaded by mononuclear phagocytic cells to a greater or lesser extent, depending on the immunogenicity of the tumour.…”

Μελετη Της in Vitro Αναπτυξης Των Προδρομων Κυτταρων Της Κοκκιωδους Σειρας Στην Οξεια Μυελογενη Λευχαιμια

“…Χημικώς είναι γλυκοπρωτεϊνες και μέχρι τώρα έχουν απομονωθεί σέ υλικό από ανθρώπινα λευκοκύτταρα τέσσερις παράγοντες μέ Μ.Β.1300, 14.700, 36.500 καί 93.000 daltons (Price et al 1975), ένώ σέ υλικό πού παρασκευάσθηκε από εμβρυϊκό πλακούντα απομονώθηκε ένας μόνο παράγοντας μέ Μ.Β. περίπου 30.000 daltons (Burgess et al 19773 (Shah et al 1978, Singer et al 1978, Eliopoulos et al 1979, Giannoulis et a 1 .1981,Reizenstein 1982.…”