Effect of Baccharis dracunculifolia D.C (Asteraceae) extracts and its isolated compounds on macrophage activation

Abstract

Baccharis dracunculifolia D.C. (Asteraceae), a shrub which grows wild in Brazil, is the main botanical source of Brazilian green propolis. Since Brazilian propolis shows an immunomodulatory activity, the goal of this work was to evaluate the action of B. dracunculifolia extracts and some of its isolated compounds on reactive oxygen intermediate (H(2)O(2)) production by macrophages obtained from male BALB/c mice. The results showed that the leaf (Bd-L) (25, 50, and 100 microg mL(-1)), leaf rinse (Bd-LR) (25 microg mL(-1)), and the root (Bd-R) (25 microg mL(-1)) extracts enhanced H2O2 release by macrophages. A phytochemical study of the root and leaves of B. dracunculifolia was carried out. The chromatographic fractionation of Bd-R, using several techniques, afforded the isolation of baccharis oxide (1), friedelanol (2), viscidone (11), 11-hydroxy-10,11-dihydro-euparin (12), and 6hydroxy-tremetona (13), while Bd-LR gave the following isolated compounds: baccharis oxide (1), friedelanol (2), isosakuranetin (3), aromadendrin-4'-methyl ether (4), dihydrocumaric acid (5), baccharin (6), hautriwaic acid lactone (7), hautriwaic acid acetate (8), drupanin (9), and cumaric acid (10). Among the isolated compounds, baccharis oxide (1) and friedelanol (2) increased H2O2 production at a concentration of 100 microM. This is the first time that the presence of compounds 7, 8, 12, and 13 in B. dracunculifolia has been reported. Based on these results it is suggested that the crude extracts and some isolated compounds from B. dracunculifolia display an immunomodulatory action.

Perfis físico-químico e cromatográfico de amostras de própolis produzidas nas microrregiões de Franca (SP) e Passos (MG), Brasil

“…Por apresentar atividades biológicas, a própolis vem sendo utilizada há milhares de anos pelo homem, para mumifi cação de cadáveres (Salatino 2005), para tratamento de infecções bacterianas (Sforcin et al, 2000) e fúngicas (Murad et al, 2002), bem como devido às atividades antiinfl amatória (Reis et al, 2000), contra afecções do trato respiratório (Tavares et al, 2006;Soares et al, 2006), antioxidante (Simões et al, 2004), imunomodulatória (Sforcin et al, 2005;Missima et al, 2006), antitumoral (Bazo et al, 2002) e antiúlcera (Barros et al, 2006).…”

In vitroantileishmanial, antiplasmodial and cytotoxic activities of a new ventiloquinone and five known triterpenes fromParinari excelsa

“…A wide variety of chemical compounds have been identified from Parinari genus, such as diterpenes and triterpenes (Uys et al 2002), flavonoids, flavonols; flavanones and quinones (Braca et al 2005;Werawattanachai et al 2010). It is well known that the chemical composition of plant can change depending on several factors, including the collection site (Missima et al 2007). The isolation of triterpenes and quinones showed that our results are in agreement with those reported in the literature.…”

Ação do extrato de própolis nas Leishmaniose

“…A própolis do Sudeste brasileiro, conhecida como própolis verde devido à sua cor, é extensivamente utilizada em alimentos e bebidas, especialmente no Brasil e no Japão, com o objetivo de melhorar a saúde e prevenir muitas doenças (MARCUCCI, 1995;MARCUCCI;DE CAMARGO;LOPES, 1996;CAMPOS et al, 1998, BANKOVA;MARCUCCI, 2000;MISSIMA et al, 2007). No Brasil, a planta mais importante como fonte de própolis verde (BGP) é a Baccharis dracunculifolia D. C. (Asteraceae) (KUMAZAWA et al, 2003).…”