Effect of hypoxia and interleukin‐1<i>β</i> on expression of tenascin‐C in temporomandibular joint

Tojyo, Itaru; Yamaguchi, Akihiko; Nitta, T.; Yoshida, Hiroaki; Fujita, Shigeyuki; Yoshida, Toshimichi

doi:10.1111/j.1601-0825.2006.01344.x

Cited by 20 publications

(20 citation statements)

References 28 publications

(33 reference statements)

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“…A disrupted nutrient supply has long been implicated in the development of IVD disc degeneration, including cartilage end-plate calcification and a further decrease in oxygen and glucose levels. In TMJ disc cells, recent studies have shown that hypoxia with inflammation modulates the gene expression of tenascin-C and matrix metalloproteinases 17, 18 . However, unlike the chondrocytes and IVD cells, the effect of essential nutrients (e.g., oxygen and glucose) on the energy metabolism and matrix synthesis of TMJ disc cells is still largely unknown.…”

Section: Introductionmentioning

confidence: 99%

The effects of oxygen level and glucose concentration on the metabolism of porcine TMJ disc cells

Cisewski

Zhang

Kuo

et al. 2015

Osteoarthritis and Cartilage

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Objective To determine the combined effect of oxygen level and glucose concentration on cell viability, ATP production, and matrix synthesis of temporomandibular joint (TMJ) disc cells. Design TMJ disc cells were isolated from pigs aged 6-8 months and cultured in a monolayer. Cell cultures were preconditioned for 48 hours with 0, 1.5, 5, or 25mM glucose DMEM under 1%, 5%, 10%, or 21% O2 level, respectively. The cell viability was measured using the WST-1 assay. ATP production was determined using the Luciferin-Luciferase assay. Collagen and proteoglycan synthesis were determined by measuring the incorporation of [2, 3-3H]proline and [35S]sulfate into the cells, respectively. Results TMJ disc cell viability significantly decreased (P<0.0001) without glucose. With glucose present, decreased oxygen levels significantly increased viability (P<0.0001), while a decrease in glucose concentration significantly decreased viability (P<0.0001). With glucose present, decreasing oxygen levels significantly reduced ATP production (P<0.0001) and matrix synthesis (P<0.0001). A decreased glucose concentration significantly decreased collagen synthesis (P<0.0001). The interaction between glucose and oxygen was significant in regards to cell viability (P<0.0001), ATP production (P=0.00015), and collagen (P=0.0002) and proteoglycan synthesis (P<0.0001). Conclusions Although both glucose and oxygen are important, glucose is the limiting nutrient for TMJ disc cell survival. At low oxygen levels, the production of ATP, collagen, and proteoglycan are severely inhibited. These results suggest that steeper nutrient gradients may exist in the TMJ disc and it may be vulnerable to pathological events that impede nutrient supply.

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Section: Introductionmentioning

confidence: 99%

The effects of oxygen level and glucose concentration on the metabolism of porcine TMJ disc cells

Cisewski

Zhang

Kuo

et al. 2015

Osteoarthritis and Cartilage

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“…The balance between the rate of nutrient transport through the matrix and the rate of consumption by disc cells establishes a concentration gradient across the disc. The concentration levels of essential nutrients, such as oxygen and glucose, can profoundly affect disc cell viability, matrix synthesis, and response to inflammatory factors (Yamaguchi et al, 2005;Tojyo et al, 2008). This suggests that deviation from physiological nutrient levels in the TMJ disc due to the lack of nutrient supply may initiate tissue remodeling and matrix degradation.…”

Section: Introduction Tmentioning

confidence: 99%

Effect of Mechanical Loading on Electrical Conductivity in Porcine TMJ Discs

et al. 2011

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The objective of this study was to examine the impact of mechanical loading on solute transport in porcine temporomandibular joint (TMJ) discs using the electrical conductivity method. The electrical conductivity, as well as ion diffusivity, of TMJ discs was determined under confined compression with 3 strains in 5 disc regions. The average electrical conductivity over the 5 regions (mean ± SD) at 0% strain was 3.10 ± 0.68 mS/cm, decreased to 2.76 ± 0.58 mS/cm (-11.0%) at 10% strain, and 2.38 ± 0.55 mS/cm (-22.2%) at 20% compressive strain. Correspondingly, the average relative ion diffusivity (mean ± SD) at 0% strain was 0.273 ± 0.055, decreased to 0.253 ± 0.048 (-7.3%) at 10% strain, and 0.231 ± 0.048 (-15.4%) at 20% compressive strain. These results indicated that compressive strain impeded solute transport in the TMJ disc. Furthermore, our results showed that the transport properties of TMJ discs were regiondependent. The electrical conductivity and ion diffusivity in the anterior region were significantly higher than in the posterior region. This regional difference is likely due to the significant differences of tissue hydration between these 2 regions. This study provides important insight into the electrical and solute transport behaviors in TMJ discs under mechanical loading and aids in the understanding of TMJ pathophysiology related to tissue nutrition.

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“…After 3, 5 and 9 days in culture, fibronectin, collagens I and IV, Lysyl Oxidase ( LOX ), Lysyl Oxidase Like-2 ( LOXL2 ) and VEGFA mRNA expression were quantified in hypoxia and atmospheric-exposed co-cultures. These genes were chosen as they have been shown to be regulated by hypoxia and play an important role in ECM organization and stability and angiogenesis [6, 7, 15-24]. We found that collagen 1αI and LOX mRNA were upregulated in response to hypoxic as compared to atmospheric conditions, while collagen IV, fibronectin, VEGFA, and LOXL2 mRNA were largely downregulated in response to hypoxic as compared to atmospheric conditions (Figure 2).…”

Section: Resultsmentioning

confidence: 94%

“…For instance, we have recently shown that hypoxia increases the expression of collagens I and IV, laminin and fibronectin deposition from ECs [15]. Similarly, others have shown using fibroblasts and animal models that hypoxia increases collagen I mRNA expression, synthesis and deposition [16-20], fibronectin protein expression [21,22], collagen IV mRNA expression and secretion [21,23], and tenascin-C mRNA and protein expression [24]. In addition to these, hypoxia has also been reported to influence the expression of prolyl hydroxylases and lysyl oxidases (LOX), enzymes which stabilize and promote crosslinking of collagens and elastins in the ECM [25].…”

Section: Introductionmentioning

confidence: 97%

Hypoxia Affects the Structure of Breast Cancer Cell-Derived Matrix to Support Angiogenic Responses of Endothelial Cells

Hielscher

Qiu

Porterfield

et al. 2013

J Carcinogene Mutagene

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Hypoxia, a common feature of the tumor environment and participant in tumor progression, is known to alter gene and protein expression of several Extracellular Matrix (ECM) proteins, many of which have roles in angiogenesis. Previously, we reported that ECM deposited from co-cultures of Neonatal Fibroblasts (NuFF) with breast cancer cells, supported 3-dimensional vascular morphogenesis. Here, we sought to characterize the hypoxic ECM and to identify whether the deposited ECM induce angiogenic responses in Endothelial Cells (ECs). NuFF and MDA-MB-231 breast cancer cells were co-cultured, subjected to alternating cycles of 24 hours of 1% (hypoxia) and 21% (atmospheric) oxygen and de-cellularized for analyses of deposited ECM. We report differences in mRNA expression profiles of matrix proteins and crosslinking enzymes relevant to angiogenesis in hypoxia-exposed co-cultures. Interestingly, overt differences in the expression of ECM proteins were not detected in the de-cellularized ECM; however, up-regulation of the cell-binding fragment of fibronecin was observed in the conditioned media of hypoxic co-cultures. Ultrastructure analyses of the de-cellularized ECM revealed differences in fiber morphology with hypoxic fibers more compact and aligned, occupying a greater percent area and having larger diameter fibers than atmospheric ECM. Examining the effect of hypoxic ECM on angiogenic responses of ECs, morphological differences in Capillary-Like Structures (CLS) formed atop de-cellularized hypoxic and atmospheric ECM were not evident. Interestingly, we found that hypoxic ECM regulated the expression of angiogenic factors and matrix metalloproteinases in CLS. Overall, we report that in vitro, hypoxia does not alter the composition of the ECM deposited by co-cultures of NuFF/MDA-MB-231, but rather alters fiber morphology, and induces vascular expression of angiogenic growth factors and metalloproteinases. Taken together, these results have important implications for understanding how the hypoxic matrix may regulate angiogenesis in tumors.

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Effect of hypoxia and interleukin‐1β on expression of tenascin‐C in temporomandibular joint

Abstract: These results indicate that hypoxic conditions with inflammation modulate the tenascin-C expression in synovial fibroblasts, but not in disk cells.

Cited by 20 publications

References 28 publications

The effects of oxygen level and glucose concentration on the metabolism of porcine TMJ disc cells

The effects of oxygen level and glucose concentration on the metabolism of porcine TMJ disc cells

Effect of Mechanical Loading on Electrical Conductivity in Porcine TMJ Discs

Hypoxia Affects the Structure of Breast Cancer Cell-Derived Matrix to Support Angiogenic Responses of Endothelial Cells

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