2013
DOI: 10.4172/2157-2518.s13-005
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Hypoxia Affects the Structure of Breast Cancer Cell-Derived Matrix to Support Angiogenic Responses of Endothelial Cells

Abstract: Hypoxia, a common feature of the tumor environment and participant in tumor progression, is known to alter gene and protein expression of several Extracellular Matrix (ECM) proteins, many of which have roles in angiogenesis. Previously, we reported that ECM deposited from co-cultures of Neonatal Fibroblasts (NuFF) with breast cancer cells, supported 3-dimensional vascular morphogenesis. Here, we sought to characterize the hypoxic ECM and to identify whether the deposited ECM induce angiogenic responses in Endo… Show more

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Cited by 17 publications
(28 citation statements)
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“…NuFF (passages 18–28) were co-cultured with MDA breast cancer cells for establishment of cell-derived ECM. Both cell lines were seeded on the same day in a 1:1 ratio in 4 well Nunc LabTek II Chamberslides (Sigma) using methods previously described [ 11 , 14 ]. In brief, co-cultures were maintained in one-half NuFF media and one-half MDA media with a final concentration of 10% fetal bovine serum (FBS).…”
Section: Methodsmentioning
confidence: 99%
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“…NuFF (passages 18–28) were co-cultured with MDA breast cancer cells for establishment of cell-derived ECM. Both cell lines were seeded on the same day in a 1:1 ratio in 4 well Nunc LabTek II Chamberslides (Sigma) using methods previously described [ 11 , 14 ]. In brief, co-cultures were maintained in one-half NuFF media and one-half MDA media with a final concentration of 10% fetal bovine serum (FBS).…”
Section: Methodsmentioning
confidence: 99%
“…Isolation of cell-derived ECM and subsequent seeding of HUVECs for vascular morphogenesis were based off of methods described in prior published reports [ 11 , 14 ]. HUVECs were used at passages 4–5 for all analyses of vascular morphogenesis on de-cellularized ECM and were cultured in EGM media containing 2% serum.…”
Section: Methodsmentioning
confidence: 99%
See 1 more Smart Citation
“…Vascular derivatives, including both EVCs and derived ECs, obtained under low-oxygen differentiation conditions demonstrated a greater propensity to produce ECM proteins collagen IV, fibronectin, and laminin. In our previous studies, we also document a correlation between hypoxic conditions and ECM production [26,30]; hypoxic exposure promoted ECM deposition from mature ECs and yielded a more fibrous ECM ultrastructure from cocultures of fibroblasts and breast cancer cells. In both our previous studies and current study, we could not observe collagen I deposition from any types of ECs tested in atmospheric conditions; however, 5% O 2 conditions stimulated collagen I production by ECs harvested from human umbilical vein or artery [26].…”
Section: Discussionmentioning
confidence: 99%
“…Advances in modeling breast cancer in vitro using multi-culture techniques has recently been reviewed (Regier et al 2016). Though less common than mono- and co-culture models, heterotypic models comprised of breast cancer cells with two or more other cell types have successfully generated in vitro functional recapitulation of in vivo processes including migration (Torisawa et al 2010), intravasation (Zervantonakis et al 2012), and extravasation (Jeon et al 2015) as well as other critical functions such as angiogenesis induction (Hielscher et al 2012; Hielscher et al 2013), and micrometastasis formation (Bersini et al 2014). However, the role of the increase in heterotypic complexity in the success of these models is difficult to define for two primary reasons.…”
Section: Introductionmentioning
confidence: 99%