EFFECT OF HYPO‐ AND HYPERTONIC SALINE AND RAFFINOSE ON CELL CONTENT OF Na, Cl and K IN RESPIRING RAT KIDNEY CORTEX AT 37°

Az, Györy; Kweifio-Okai, George; Ng, J.

doi:10.1038/icb.1981.14

Cited by 5 publications

(1 citation statement)

References 18 publications

(43 reference statements)

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“…Our previous work on kidney slices incubated at 37OC showed complete cell volume regulation (Gyory ef al. 1981b) following ouabain, despite an increase in "a], which was counterbalanced by loss of Ki (Gyory et al 1981a).…”

Section: Discussionmentioning

confidence: 96%

Effect of Atrial Natriuretic Peptide on Cellular Element Concentrations in Rat Proximal Tubules: Evidence for Inhibition of the Sodium Pump

Reddy

Györy

Dyne

et al. 1994

Clin Exp Pharma Physio

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1. In order to further define the action of atrial natriuretic peptide (ANP) on proximal tubular (PT) transport, combined clearance and electron microprobe X-ray (EMPX) experiments were performed on five male Wistar rats infused with ANP (0.16 nmol/kg per h) and nine control animals. 2. Electron microprobe X-ray analysis of PT cell electrolytes (mmol/kg wet weight) revealed a similar [Na]i in both the control and ANP treated groups (16.4 +/- 0.4 vs 16.5 +/- 0.4; P = 0.894). [Cl]i was lower in the ANP treated animals (14.8 +/- 0.3 vs 12.0 +/- 0.3; P < 0.0001) as was [K]i (131.4 +/- 1.4 vs 114 +/- 1.7; P < 0.0001). The PT cells in the ANP treated group had a significant reduction in dry weight (20.1 +/- 0.3 g% vs 19.0 +/- 0.3 g%; P < 0.024), indicating significant cell swelling. Thus, despite a normal [Na]i, there was net accumulation of Nai following ANP treatment. 3. These results are consistent with accumulation of Nai due to inhibition of the Na pump followed by cell swelling and subsequent regulatory volume decrease with exit of K and Cl. These results are the first to show the effect of ANP on PT intracellular electrolytes.

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Section: Discussionmentioning

confidence: 96%

Effect of Atrial Natriuretic Peptide on Cellular Element Concentrations in Rat Proximal Tubules: Evidence for Inhibition of the Sodium Pump

Reddy

Györy

Dyne

et al. 1994

Clin Exp Pharma Physio

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Electron microprobe analysis of proximal tubule cellular Na, Cl and K element concentrations during acute mannitol-saline volume expansion in rats: evidence for inhibition of the Na pump

Györy¹,

Beck

Rick

et al. 1985

Pflugers Arch.

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It has previously been shown that during mannitol-saline volume expansion (VE) Na transport was inhibited 50% by harvested proximal tubular fluid without a change in paracellular shunt pathway permeability to Na. To determine whether this inhibition was due to changes in cellular entry step or an effect on the pump itself, intracellular element concentrations were measured by electron microprobe X-ray ranalysis in proximal tubular cells of control (non-expanded, NE) and VE rats. Nai, Cli and phosphorusi were increased (mean +/- S.E.) from 19.3 +/- 0.8 to 23.4 +/- 0.6, 15.8 +/- 0.4 to 21.3 +/- 0.4 and 124.3 +/- 2.6 to 138.0 +/- 1.8 mmol . kg-1 wet weight (P less than 0.001) respectively while Ki remained unchanged: 122.9 +/- 2.2 and 124.2 +/- 1.3 mmol . kg-1 wet weight. The increases in Nai and Cli were in excess of cell shrinkage produced by the hyperosmolal peritubular environment while the unchanged Ki in the face of cell shrinkage indicates and actual loss. It is concluded that mannitol-saline VE inhibits the Na pump producing a rise in Nai and a fall in Ki.

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Electron probe X‐ray microanalysis of intracellular element concentrations in cryosections in the presence of changes in cell volume

Boström

Field

Györy

et al. 1991

Journal of Microscopy

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SUMMARY The interpretation of element concentration data for X‐ray microanalyses of biological tissues, which are subjected to some experimental treatment, can be complicated by changes in cell volume and total cell dry matter induced by the treatment. We have examined the manner in which such changes would affect the values measured in frozen‐dried cryosections of soft tissues, and how they may be taken into account in the interpretation of the results. The element content (mass per unit dry weight) measured by the peak‐to‐continuum or Hall method is independent of changes in cell volume, but is sensitive to a change in the local dry mass. Conversely, intracellular concentrations in terms of mass per unit volume, as determined by the peripheral or internal standard technique, are dependent on volume changes but independent of dry mass. The estimated dry weight fraction is affected by changes in both volume and dry mass. The results obtained from both quantification methods can therefore provide information on the combination of changes in cellular element levels, volume and total dry mass that may occur following the experimental treatment. In a study of the late effect of the drug cisplatin on electrolyte concentrations in kidney proximal tubules, both quantification methods have been used to obtain wet weight and dry weight concentrations. By applying the above considerations, the analytical results have been interpreted as a combination of changes in element levels and a shrinkage of the tubule cells. Cell shrinkage was confirmed by morphometric analysis of tubular cross‐sections.

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EFFECT OF HYPO‐ AND HYPERTONIC SALINE AND RAFFINOSE ON CELL CONTENT OF Na, Cl and K IN RESPIRING RAT KIDNEY CORTEX AT 37°

Cited by 5 publications

References 18 publications

Effect of Atrial Natriuretic Peptide on Cellular Element Concentrations in Rat Proximal Tubules: Evidence for Inhibition of the Sodium Pump

Effect of Atrial Natriuretic Peptide on Cellular Element Concentrations in Rat Proximal Tubules: Evidence for Inhibition of the Sodium Pump

Electron microprobe analysis of proximal tubule cellular Na, Cl and K element concentrations during acute mannitol-saline volume expansion in rats: evidence for inhibition of the Na pump

Electron probe X‐ray microanalysis of intracellular element concentrations in cryosections in the presence of changes in cell volume

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