Effect of hypo‐ and hyperthyroidism on the function and metabolism of macrophages in rats

Rosa, L. F. B. P. Costa; Safi, D A; Curi, Rui

doi:10.1002/cbf.290130211

Cited by 32 publications

(23 citation statements)

References 25 publications

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“…In rat macrophages, hyperthyroidism was shown to increase the rates of glucose uptake (28). In these cells, glycolysis was evaluated by measuring the activity of hexokinase and Krebs cycle by measuring the activity of citrate synthase and the decarboxylation of [U- 14 C]glucose (27)(28)(29). In macrophages from normal rats, insulin was shown to increase the activities of hexokinase and citrate synthase as well as the decarboxylation of glucose; in contrast, in macrophages from hyperthyroid rats, the activity of hexokinase was increased, whereas both the activity of citrate synthase and the decarboxylation of [U- 14 C]glucose were decreased suggesting an increase in the rates of glycolysis and a decrease in the rates of glucose oxidation (27)(28)(29).…”

Section: Effects Of Igf-i On Glut Isoformsmentioning

confidence: 95%

“…Several studies have shown that monocytes and macrophages utilize glucose at high rates and their metabolism is modified by insulin and thyroid hormones (25)(26)(27)(28)(29). In rat macrophages, hyperthyroidism was shown to increase the rates of glucose uptake (28).…”

Section: Effects Of Igf-i On Glut Isoformsmentioning

confidence: 99%

Section: Effects Of Igf-i On Glut Isoformsmentioning

confidence: 99%

“…In these cells, glycolysis was evaluated by measuring the activity of hexokinase and Krebs cycle by measuring the activity of citrate synthase and the decarboxylation of [U- 14 C]glucose (27)(28)(29). In macrophages from normal rats, insulin was shown to increase the activities of hexokinase and citrate synthase as well as the decarboxylation of glucose; in contrast, in macrophages from hyperthyroid rats, the activity of hexokinase was increased, whereas both the activity of citrate synthase and the decarboxylation of [U- 14 C]glucose were decreased suggesting an increase in the rates of glycolysis and a decrease in the rates of glucose oxidation (27)(28)(29). These results are in agreement with the experiments performed in the skeletal muscle of rats, which were made hyperthyroid after treatment with triiodothyronine for 2, 5, or 10 days: although the sensitivity of glycogen synthesis to insulin was clearly decreased and that of lactate formation was increased after all three experimental conditions, the sensitivity of glucose oxidation to insulin was increased only after 5 or 10 days treatment, suggesting that in hyperthyroidism, there is a preferential increase in glycolysis and lactate formation relative to glucose oxidation and storage in skeletal muscle (30).…”

Section: Effects Of Igf-i On Glut Isoformsmentioning

confidence: 99%

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IGF-I increases the recruitment of GLUT4 and GLUT3 glucose transporters on cell surface in hyperthyroidism

Dimitriadis

Maratou²,

Boutati³

et al. 2008

European Journal of Endocrinology

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Objective: In hyperthyroidism, tissue glucose disposal is increased to adapt to high energy demand. Our aim was to examine the regulation of glucose transporter (GLUT) isoforms by IGF-I in monocytes from patients with hyperthyroidism. Design and methods: Blood (20 ml) was drawn from 21 healthy and 10 hyperthyroid subjects. The abundance of GLUT isoforms on the monocyte plasma membrane was determined in the absence and presence of IGF-I (0.07, 0.14, and 0.7 nM) using flow cytometry. Anti-CD14-phycoerythrin monocional antibody was used for monocyte gating. GLUT isoforms were determined after staining the cells with specific antisera to GLUT3 and GLUT4. Results: In monocytes from the euthyroid subjects, IGF-I increased the abundance of GLUT3 and GLUT4 on the monocyte surface by 25 and 21% respectively (P!0.0005 with repeated measures ANOVA). Hyperthyroidism increased the basal monocyte surface GLUT3 and GLUT4; in these cells, IGF-I had a marginal but highly significant effect (PZ0.003, with repeated measures ANOVA) on GLUT3 (11%) and GLUT4 (10%) translocation on the plasma membrane. Conclusions: In hyperthyroidism: 1) basal abundance of GLUT3 and GLUT4 on the plasma membrane is increased and 2) the sensitivity of the recruitment of GLUT3 and GLUT4 transporters on the plasma membrane in response to IGF-I is increased. These findings may contribute to the understanding of the mechanism by which hyperthyroidism increases glucose disposal in peripheral tissues.

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Section: Effects Of Igf-i On Glut Isoformsmentioning

confidence: 95%

Section: Effects Of Igf-i On Glut Isoformsmentioning

confidence: 99%

Section: Effects Of Igf-i On Glut Isoformsmentioning

confidence: 99%

Section: Effects Of Igf-i On Glut Isoformsmentioning

confidence: 99%

See 2 more Smart Citations

IGF-I increases the recruitment of GLUT4 and GLUT3 glucose transporters on cell surface in hyperthyroidism

Dimitriadis

Maratou²,

Boutati³

et al. 2008

European Journal of Endocrinology

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“…Several studies in both human subjects and animal models have found that, similar to neutrophils, stimulated hyperthyroid macrophages have increased ROS production ex vivo (Videla et al 1993, Magsino et al 2000 although one study found an opposite effect (Rosa et al 1995). PTU treatment of hyperthyroid patients normalized ROS production although these findings could not be replicated in vitro (Videla et al 1993).…”

Section: Effects Of Extracellular Thyroid Hormone Levels On Macrophagmentioning

confidence: 99%

Thyroid hormone metabolism in innate immune cells

Spek

Fliers

Boelen

2017

Journal of Endocrinology

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Thyroid hormone (TH) metabolism and thyroid status have been linked to various aspects of the immune response. There is extensive literature available on the effects of thyroid hormone on innate immune cells. However, only recently have authors begun to study the mechanisms behind these effects and the role of intracellular TH metabolism in innate immune cell function during inflammation. This review provides an overview of the molecular machinery of intracellular TH metabolism present in neutrophils, macrophages and dendritic cells and the role and effects of intracellular TH metabolism in these cells. Circulating TH levels have a profound effect on neutrophil, macrophage and dendritic cell function. In general, increased TH levels result in an amplification of the pro-inflammatory response of these cells. The mechanisms behind these effects include both genomic and non-genomic effects of TH. Besides a pro-inflammatory effect induced by extracellular TH, the cellular response to pro-inflammatory stimuli appears to be dependent on functional intracellular TH metabolism. This is illustrated by the fact that the deiodinase enzymes and in some cell types also thyroid hormone receptors appear to be crucial for adequate innate immune cell function. This overview of the literature suggests that TH metabolism plays an important role in the host defence against infection through the modulation of innate immune cell function.

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Gonadectomy impairs lymphocyte proliferation and macrophage function in male and female rats. Correlation with key enzyme activities of glucose and glutamine metabolism

Azevedo

Rosa

Lacava

et al. 1997

Cell Biochem. Funct.

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The effect of gonadectomy on lymphocyte proliferation and macrophage function (hydrogen peroxide production and phagocytosis capacity) of male and female rats was examined and the results correlated with the activities of hexokinase, glucose-6-phosphate dehydrogenase, citrate synthase and phosphate-dependent glutaminase. Also, the reversion of the changes by the treatment with oestrogen or progesterone or a combination of both was addressed. Taken as a whole, ovariectomy reduced hydrogen peroxide production and phagocytosis capacity by macrophages and also lymphocyte proliferation. Castration of male rats reduced the proliferation of lymphocytes and raised macrophage phagocytosis capacity. The effects on macrophage function were correlated with changes in glucose metabolism, particularly, in the activity of glucose-6-phosphate dehydrogenase.

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Effect of hypo‐ and hyperthyroidism on the function and metabolism of macrophages in rats

Cited by 32 publications

References 25 publications

IGF-I increases the recruitment of GLUT4 and GLUT3 glucose transporters on cell surface in hyperthyroidism

IGF-I increases the recruitment of GLUT4 and GLUT3 glucose transporters on cell surface in hyperthyroidism

Thyroid hormone metabolism in innate immune cells

Gonadectomy impairs lymphocyte proliferation and macrophage function in male and female rats. Correlation with key enzyme activities of glucose and glutamine metabolism

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