Effect of hyperphosphatemia on gene expression of the Na-Pi cotransporter in rats

Zhang, C.; Shi, Linlin; Li, Y.; Zhu, Qing; Jin, Chengluo; Wang, H.-P.; Zhou, Li; Hu, Xiaoqi; Zhang, L.; Wang, H.; Yuan, Guan; Xu, Lubin; Zhao, Yan-yan; Chou, Yah‐Huei Wu

doi:10.4238/2015.december.30.1

Cited by 2 publications

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“…Phosphorus, an indispensable element in the growth and development of animals, plays an important role in bone formation, energy metabolism, nucleic acid synthesis, cell signal transduction, and maintenance of blood acid-base balance ( Wagner et al., 2014 , Miao et al., 2017 ). Phosphorus reabsorption in the kidney depends on the NaPi-IIa and NaPi-IIc cotransporter, and the absorption in the intestine depends on the NaPi-IIb cotransporter, which are affected by the mRNA expressions of related genes and hyperphosphatemia ( Virkki et al., 2007 , Biber et al., 2009 , Zhang et al., 2016 , Sugiura et al., 2018 ). Prebiotics have been proven to improve the intestinal transport of calcium and protein in rats, however the effect of supplementation of infant formula with prebiotics on phosphorus absorption has rarely been studied.…”

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confidence: 99%

Enzyme activity and phosphate uptake in the small intestine of Sprague Dawley rats improved by supplementation of infant formula with prebiotics

et al. 2018

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This study was to identify the effects of prebiotics supplemented in infant formula on enzyme activity and phosphate uptake in the small intestine of Sprague Dawley (SD) rats. Forty-eight healthy SD rats at 15 days old (a week before weaning) with similar weight were randomly divided into 3 groups: A (control group), B, C, with 16 rats per group. Rats in groups A, B, C were fed a standard infant formula, the standard infant formula supplemented with oligosaccharides, and the standard infant formula supplemented with polysaccharides, respectively. The feeding test was conducted for 28 d. Compared with group A, the results showed the following: 1) the activities of sucrose and lactase in the small intestine were significantly increased in SD rats of group C (P < 0.05); 2) the relative expressions of lactase gene in the anterior and posterior segments of the small intestine were significantly increased by 1.68 and 2.26 in SD rats of group C (P < 0.05), and the relative expression of Mgam gene in the posterior segment of the small intestine was significantly increased by 0.99 in SD rats of group C (P < 0.05); 3) the relative expressions of Na/Pi-IIb gene in the anterior and posterior segments of the small intestine were significantly increased by 1.85 and 2.28 in SD rats of group C (P < 0.05). These results indicate that the supplementation of infant formula with prebiotics can promote enzyme activity in the small intestine by increasing the relative expression of enzyme gene or by decreasing intestinal injury, and can increase the relative expression of Na/Pi-IIb gene. The effect of polysaccharides is better than that of oligosaccharides.

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Section: Discussionmentioning

confidence: 99%

Enzyme activity and phosphate uptake in the small intestine of Sprague Dawley rats improved by supplementation of infant formula with prebiotics

et al. 2018

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Phosphate Transport in Epithelial and Nonepithelial Tissue

Hernando

Gagnon

Lederer

2021

Physiological Reviews

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Phosphate is an essential nutrient for life and is a critical component of bone formation, a major signaling molecule, and structural component of cell walls. Phosphate is also a component of high-energy compounds (i.e. AMP, ADP, and ATP), and essential for nucleic acid helical structure (i.e. RNA and DNA). Phosphate plays a central role in the process of mineralization, normal serum levels being associated with appropriate bone mineralization, while high and low serum levels are associated with soft tissue calcification. The serum concentration of phosphate and the total body content of phosphate are highly regulated, a process that is accomplished by the coordinated effort of two families of sodium-dependent transporter proteins. The three isoforms of the SLC34 family (SLC34A1-A3) show very restricted tissue expression and regulate intestinal absorption and renal excretion of phosphate. SLC34A2 also regulates the phosphate concentration in multiple lumen fluids including milk, saliva, pancreatic fluid, and surfactant. Both isoforms of the SLC20 family exhibit ubiquitous expression (with some variation as to which one or both are expressed), are regulated by ambient phosphate, and likely serve the phosphate needs of the individual cell. These proteins exhibit similarities to phosphate transporters in non-mammalian organisms. The proteins are non-redundant as mutations in each yield unique clinical presentations. Further research is essential to understand the function, regulation, and coordination of the various phosphate transporters, both the ones described in this review and the phosphate transporters involved in intracellular transport.

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Effect of hyperphosphatemia on gene expression of the Na-Pi cotransporter in rats

Cited by 2 publications

References 8 publications

Enzyme activity and phosphate uptake in the small intestine of Sprague Dawley rats improved by supplementation of infant formula with prebiotics

Enzyme activity and phosphate uptake in the small intestine of Sprague Dawley rats improved by supplementation of infant formula with prebiotics

Phosphate Transport in Epithelial and Nonepithelial Tissue

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