Effect of hyperbaric oxyhelium gas on response of bacteria to antimicrobial agents in vitro

Abstract

Modern diving techniques can require the treatment of infection in an atmosphere of pressurized oxyhelium gas. The antibiotic susceptibility of 16 species and strains (eight genera) of gram-negative bacilli and 3 species and strains (two genera) of gram-positive cocci to each of 21 antimicrobial agents was assessed in air at atmospheric pressure and in oxyhelium gas at an absolute pressure of 7 bar (ca. 709 kPa). A disk diffusion technique was employed, and significantly different results were obtained in the two atmospheres. The effect of oxyhelium on diameters of growth inhibition varied significantly with the bacterium and with the antibiotic and was particularly marked with certain bacterium-antibiotic combinations. The gram-negative bacilli generally gave reduced zone diameters in oxyhelium, whereas the gram-positive cocci showed a mixture of effects.

Oxygen in acute and chronic wound healing

“… 53 demonstrated that oxygen triggers the differentiation of fibroblasts to myofibroblasts – a possible explanation for the accelerated healing. It has also been shown in vitro that HBOT may increase the susceptibility of certain bacteria to antimicrobial agents 120 . For example, Kenward et al.…”

“…It has also been shown in vitro that HBOT may increase the susceptibility of certain bacteria to antimicrobial agents. 120 For example, Kenward et al 120 reported that under HBOT the zones of inhibition were reduced in Gram-negative bacterial cultures, whereas for Gram-positive bacteria a mixture of effects was found. It seems that these effects are quite strain specific and may not easily be generalized to all aerobic or anaerobic bacteria.…”

Uso de oxígeno hiperbárico para el manejo de heridas: bases físicas, biológicas y evidencia disponible

“…De esta manera aumenta la eficiencia del proceso de limpieza de restos celulares y la defensa contra microorganismos que pudieran enlentecer la cicatrización 33,34 . Este factor tiene especial relevancia en individuos en que su sistema inmune está comprometido, como por ejemplo: pacientes diabéticos, en tratamiento inmunosupresor y con inmunodeficiencias congénitas o adquiridas 35 .…”

The role of oxygen‐associated therapies for the healing of chronic wounds, particularly in patients with diabetes

“…The researchers found that using four sessions of HBOT, positive bacterial cultures from the lesions significantly decreased (from 19 to 3 in the study group against 16-12 in the conventional treatment group). In addition, Kenward et al 43 demonstrated the significant effect of HBOT on in vitro Gram-negative bacteria cultures.…”

“…In addition, Kenward et al. 43 demonstrated the significant effect of HBOT on in vitro Gram‐negative bacteria cultures.…”