Effect of hydroxypropyl‐β‐cyclodextrin in fluid and semi‐solid submicron emulsions on physiological skin parameters during regular in vivo application

Pany, Astrid; Wohlgenannt, Marie; Klopprogge, Safoura; Wolzt, Michael; Heuser, Thomas; Kotisch, Harald; Valenta, Claudia; Klang, Victoria

doi:10.1111/ics.12674

Cited by 4 publications

(6 citation statements)

References 26 publications

(26 reference statements)

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“…All the measured NMF profiles had this same trend; however, the SDS and LPC80 profiles started at a lower NMF concentration, leading to overall lower NMF levels. These findings are consistent with the literature [33,47,58]. These significant drops in NMF levels could be due to the denaturation of proteins of the cornified envelope, which may lead to leakage of NMF components [59,60].…”

Section: Discussionsupporting

confidence: 92%

Changes in Skin Barrier Function after Repeated Exposition to Phospholipid-Based Surfactants and Sodium Dodecyl Sulfate In Vivo and Corneocyte Surface Analysis by Atomic Force Microscopy

et al. 2021

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(1) Background: The aim of the study was to evaluate the effect of pure lecithins in comparison to a conventional surfactant on skin in vivo. (2) Methods: Physiological skin parameters were evaluated at the beginning and the end of the study (day 1 and day 4) (n = 8, healthy forearm skin) with an Aquaflux®, skin-pH-Meter, Corneometer® and an Epsilon® sensor. Confocal Raman spectroscopy was employed to monitor natural moisturizing factor, urea and water content of the participants’ skin. Tape strips of treated skin sites were taken and the collected corneocytes were subjected to atomic force microscopy. Circular nano objects were counted, and dermal texture indices were determined. (3) Results: Transepidermal water loss was increased, and skin hydration was decreased after treatment with SDS and LPC80. Natural moisturizing factor and urea concentrations within the outermost 10 µm of the stratum corneum were lower than after treatment with S75 or water. Dermal texture indices of skin treated with SDS were higher than skin treated with water (control). (4) Conclusions: Results suggest very good (S75) or good (LPC80) skin-tolerability of lecithin-based surfactants in comparison to SDS and encourage further investigation.

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Section: Discussionsupporting

confidence: 92%

Changes in Skin Barrier Function after Repeated Exposition to Phospholipid-Based Surfactants and Sodium Dodecyl Sulfate In Vivo and Corneocyte Surface Analysis by Atomic Force Microscopy

et al. 2021

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“…Moreover, the device offers a linearized calibrated response, unlike conventional corneometers 21 . Areas with no electric permittivity such as furrows are illustrated in black, while bright areas indicate high permittivity and therefore high skin hydration 18 . Measurements were performed in triplicate.…”

Section: Methodsmentioning

confidence: 99%

“…To answer our questions, classic biophysical skin parameters were measured, 18 such as skin hydration (Corneometer, Epsilon sensor), pH of the stratum corneum surface, transepidermal water loss (TEWL, by Aquaflux) to assess dermal barrier function. To characterize the skin composition, 6 important components (ceramide, keratin, cholesterol, natural moistorizing factor, urea and water) were observed in the skins using CRS.…”

Section: Introductionmentioning

confidence: 99%

“…To achieve our goals we performed a comparative analysis of (1) frozen and fresh skin samples, (2) young and aged skin samples, (3) abdominal skins from different species, (4) pig skins from a local market and from a veterinary farm; in addition, (5) the gradual effect of a routinely used mechanical skin pretreatment method (adhesive tape stripping) on the morphological characteristics of the skin surface was tested. To answer our questions, classic biophysical skin parameters were measured, 18 such as skin hydration (Corneometer, Epsilon sensor), pH of the stratum corneum surface, transepidermal water loss (TEWL, by Aquaflux) to assess dermal barrier function. To characterize the skin composition, 6 important components (ceramide, keratin, cholesterol, natural moistorizing factor, urea and water) were observed in the skins using CRS.…”

Section: Introductionmentioning

confidence: 99%

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Characterization and ex vivo evaluation of excised skin samples as substitutes for human dermal barrier in pharmaceutical and dermatological studies

Kocsis

Klang

Schweiger

et al. 2022

Skin Research and Technology

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Background Excised animal and human skins are frequently used in permeability testing in pharmaceutical research. Several factors exist that may have influence on the results. In the current study some of the skin parameters that may affect drug permeability were analysed for human, mouse, rat and pig skin. Materials and methods Classic biophysical skin parameters were measured (e.g. pH, hydration, permittivity, transepidermal water loss). Physiological characteristics of the skins were also analysed by confocal Raman spectroscopy, scanning electron microscopy and two‐photon microscopy. Results Based on biophysical testing, skin barrier function was damaged in psoriatic mouse skin and in marketed pig skin. Hydration and pH values were similar among the species, but freezing and thawing reduced the water content of the skins and shifted the surface pH to acidic. Aging reduced hydration and permittivity, resulting in impaired barrier function. Mechanical sensitization used in permeability studies resulted in proportional thinning of dead epidermis. Discussion Results indicate that depending on the scientific question it should be considered whether fresh or frozen tissue is used, and for certain purposes rodent skins are well usable. The structure of the skin tissue (ceramide, cholesterol, keratin, natural moisturizing factor or urea) is similar in rats and mice, but due to the higher skin thickness the lipid distribution is different in porcine skin. Psoriasis led to irregular chemical composition of the skin. Conclusion A comprehensive evaluation of skin samples of four species was performed. The biophysical and microscopic observations should be considered when selecting drug penetration models and experimental conditions.

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“…Based on the results, the authors concluded that both extracts can be considered as ingredients of skincare products for adults. Pany and co‐workers (2021) 143 tested the effect of hydroxypropyl‐β‐cyclodextrin (HP‐β‐CD) in cosmetic submicron emulsions and submicron emulsion gels on physiological skin parameters during regular application in a clinical setup. Confocal Raman spectroscopy was employed to monitor urea and NMF levels.…”

Section: Utilization Of Raman Spectroscopy In Skin Researchmentioning

confidence: 99%

Novel aspects of Raman spectroscopy in skin research

Lunter

Klang

Kocsis

et al. 2022

Experimental Dermatology

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The analytical technology of Raman spectroscopy has an almost 100‐year history. During this period, many modifications and developments happened in the method like discovery of laser, improvements in optical elements and sensitivity of spectrometer and also more advanced light detection systems. Many types of the innovative techniques appeared (e.g. Transmittance Raman spectroscopy, Coherent Raman Scattering microscopy, Surface‐Enhanced Raman scattering and Confocal Raman spectroscopy/microscopy). This review article gives a short description about these different Raman techniques and their possible applications. Then, a short statistical part is coming about the appearance of Raman spectroscopy in the scientific literature from the beginnings to these days. The third part of the paper shows the main application options of the technique (especially confocal Raman spectroscopy) in skin research, including skin composition analysis, drug penetration monitoring and analysis, diagnostic utilizations in dermatology and cosmeto‐scientific applications. At the end, the possible role of artificial intelligence in Raman data analysis and the regulatory aspect of these techniques in dermatology are briefly summarized. For the future of Raman Spectroscopy, increasing clinical relevance and in vivo applications can be predicted with spreading of non‐destructive methods and appearance with the most advanced instruments with rapid analysis time.

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Effect of hydroxypropyl‐β‐cyclodextrin in fluid and semi‐solid submicron emulsions on physiological skin parameters during regular in vivo application

Cited by 4 publications

References 26 publications

Changes in Skin Barrier Function after Repeated Exposition to Phospholipid-Based Surfactants and Sodium Dodecyl Sulfate In Vivo and Corneocyte Surface Analysis by Atomic Force Microscopy

Changes in Skin Barrier Function after Repeated Exposition to Phospholipid-Based Surfactants and Sodium Dodecyl Sulfate In Vivo and Corneocyte Surface Analysis by Atomic Force Microscopy

Characterization and ex vivo evaluation of excised skin samples as substitutes for human dermal barrier in pharmaceutical and dermatological studies

Novel aspects of Raman spectroscopy in skin research

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