Effect of humidity on melt electrospun polycaprolactone scaffolds

Liao, Sam; Langfield, Brendan; Ristovski, Nikola; Theodoropoulos, Christina; Hardt, Jake; Blackwood, Keith A.; Yambem, Soniya D.; Gregory, Shaun D.; Woodruff, Maria A.; Powell, Sean K.

doi:10.1515/bnm-2016-0009

Cited by 4 publications

(4 citation statements)

References 27 publications

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“…[ 67 ] In a recent publication, commercially available MEW printers have been retrofitted with tubular collectors to generate tubular constructs, presenting a lower barrier of entry for laboratories already utilizing one of the described systems. [ 146 ] Noteworthy is also the option of using a traditional filament‐based extrusion system compared to the widely used cartridge‐based systems. This opens up the possibility to process many more readily available thermoplastic polymers manufactured to be consistent with medical grade standards like Polydioxanone, PLLA, Poly( L ‐lactide‐ co ‐glycolide) [ 147 ] thermoplastic co‐polyester elastomer, glycol‐modified polyethene terephthalate [ 148 ] or heavy‐duty polymers like polyether ether ketone.…”

Section: The Future Of Tubular Melt Electrowriting For Biomimetic Vas...mentioning

confidence: 99%

The Past, Present, and Future of Tubular Melt Electrowritten Constructs to Mimic Small Diameter Blood Vessels – A Stable Process?

Bartolf‐Kopp,

Jungst

2024

Adv Healthcare Materials

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Melt Electrowriting (MEW) is a continuously growing manufacturing platform. Its advantage is the consistent production of micro‐ to nanometer fibers, that stack intricately, forming complex geometrical shapes. MEW allows tuning of the mechanical properties of constructs via the geometry of deposited fibers. Due to this, MEW can create complex mechanics only seen in multi‐material compounds and serve as guiding structures for cellular alignment. The advantage of MEW is also shown in combination with other biotechnological manufacturing methods to create multilayered constructs that increase mechanical approximation to native tissues, biocompatibility, and cellular response. These features make MEW constructs a perfect candidate for small‐diameter vascular graft structures. Recently, studies have presented fascinating results in this regard, but is this truly the direction that tubular MEW will follow or are there also other options on the horizon? This perspective will explore the origins and developments of tubular MEW and present its growing importance in the field of artificial small‐diameter vascular grafts with mechanical modulation and improved biomimicry and the impact of it in convergence with other manufacturing methods and how future technologies like AI may influence its progress.

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Section: The Future Of Tubular Melt Electrowriting For Biomimetic Vas...mentioning

confidence: 99%

The Past, Present, and Future of Tubular Melt Electrowritten Constructs to Mimic Small Diameter Blood Vessels – A Stable Process?

Bartolf‐Kopp,

Jungst

2024

Adv Healthcare Materials

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“…[ 32 ] A more recent study claimed that relative humidity did not play a significant role in inter‐layering fiber ordering, nor did it affect fiber diameter consistency or fiber surface morphology. [ 33 ] The fact that this difference exists within MES emphasizes that it is distinct from MEW, even though the two techniques have identical configurations.…”

Section: A Mew Handbook: Exploring the Relationships Between Processi...mentioning

confidence: 99%

A Decade of Melt Electrowriting

O'Neill

Dalton

2023

Small Methods

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Over the past decade, melt electrowriting (MEW) has established the fundamental understanding of processing (and printer) requirements. Iterative work on parametric development and dissemination of this recent additive manufacturing technology has been performed across many systems and polymers (mainly poly-(𝝐-caprolactone)), showing similarities and trends. However, the software and hardware ecosystems of MEW are not mature. Further, due to its multi-parametric nature, MEW can be challenging for laboratories to master. This review intends to provide a unique perspective on the dynamic relationship between MEW processing parameters. Such parameters can be divided into 1) those that affect the polymer flow rate to or 2) from the nozzle, and 3) environmental conditions. The most influential parameters for high-quality printing are applied voltage, applied pressure, collector speed, polymer temperature, nozzle diameter, and the conditions that lead to charge buildup (e.g., relative humidity). Other factors such as ambient temperature, nozzle size, and protrusion, collector temperature and conductivity, and collector distance can all affect the process. Success for MEW printing means fibers fall onto the collector according to their pre-programmed path with predicted fiber diameter. Here, the authors elucidate how the dynamic relationship between these parameters can converge into ideal printing conditions to produce scaffolds.

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“…While humidity was kept within a certain range (25–40% r.h.), a previous study shows that such small differences at room temperature do not have a substantial impact on MEW. [ 19 ]…”

Section: Effect Of Polymer Melt Temperature On Fiber Suspensionmentioning

confidence: 99%

Design of Suspended Melt Electrowritten Fiber Arrays for Schwann Cell Migration and Neurite Outgrowth

Hrynevich

Achenbach

Jüngst

et al. 2021

Macromolecular Bioscience

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In this study, well-defined, 3D arrays of air-suspended melt electrowritten fibers are made from medical grade poly(ɛ-caprolactone) (PCL). Low processing temperatures, lower voltages, lower ambient temperature, increased collector distance, and high collector speeds all aid to direct-write suspended fibers that can span gaps of several millimeters between support structures. Such processing parameters are quantitatively determined using a "wedge-design" melt electrowritten test frame to identify the conditions that increase the suspension probability of long-distance fibers. All the measured parameters impact the probability that a fiber is suspended over multimillimeter distances. The height of the suspended fibers can be controlled by a concurrently fabricated fiber wall and the 3D suspended PCL fiber arrays investigated with early post-natal mouse dorsal root ganglion explants. The resulting Schwann cell and neurite outgrowth extends substantial distances by 21 d, following the orientation of the suspended fibers and the supporting walls, often generating circular whorls of high density Schwann cells between the suspended fibers. This research provides a design perspective and the fundamental parametric basis for suspending individual melt electrowritten fibers into a form that facilitates cell culture.

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Effect of humidity on melt electrospun polycaprolactone scaffolds

Cited by 4 publications

References 27 publications

The Past, Present, and Future of Tubular Melt Electrowritten Constructs to Mimic Small Diameter Blood Vessels – A Stable Process?

The Past, Present, and Future of Tubular Melt Electrowritten Constructs to Mimic Small Diameter Blood Vessels – A Stable Process?

A Decade of Melt Electrowriting

Design of Suspended Melt Electrowritten Fiber Arrays for Schwann Cell Migration and Neurite Outgrowth

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