Effect of human sperm freezing-thawing process on vitrification and conventional freezing: evaluation of survival, motility, DNA oxidation and mitochondrial activity

Pabón, D.; Meseguer, M.; Sevillano, G.; Cobo, Ana; Romero, Jaime; Sota, N.; Mifsud, Amparo; Santos, Joana; Remohı́, José; Santos, M. de los

doi:10.1016/j.fertnstert.2015.07.853

Cited by 3 publications

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“…Studies have indicated no significant differences in sperm parameters between vitrified and frozen spermatozoa, 24,25 although some authors suggest vitrified samples exhibit increased sperm motility after warming. 15,[26][27][28][29] Therefore, after considering cryopreservation outcomes, in combination with the technical characteristics of each cryopreservation technique, it can be concluded that the utility of vitrification as a sperm cryopreservation option to be used routinely in clinical practice is still lacking evidence, since robust differences between sperm freezing and vitrification, in terms of the sample quality after cryopreservation, cannot yet be established. In contrast, slow freezing offers good thawing outcomes, in terms of sperm quality, as has been reported in the comparative studies summarised in Table 2.…”

Section: Dry Ice Freezingmentioning

confidence: 99%

Description and Outcomes of Current Clinical Techniques for Sperm Cryopreservation

Rios

Botella

2019

EMJ Repro Health

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Nowadays, sperm cryopreservation is strongly recommended in cases of malignancy. Moreover, the use of frozen testicular sperm in azoospermic patients prevents the need for repeated sperm retrieval and optimises scheduling between oocyte and sperm obtainment. Even though cryopreservation of human spermatozoa for assisted reproductive purposes is a widely implemented practice, none of the established freezing and vitrification techniques offer optimal cryosurvival results due to the dramatic impact of cryodamage on sperm cells. This comprehensive review describes the most commonly used sperm cryopreservation techniques in order to establish which of them minimise sperm cryodamage and offer better survival rates. Presently, it is not sufficiently demonstrated that sperm vitrification improves survival significantly more than freezing methods. Slow freezing offers the best survival results when compared to other freezing protocols, and owing to its technical advantages, can be considered as one of the preferred protocols to be easily implemented in assisted reproduction laboratories. Moreover, several studies have suggested that sperm preparation prior to cryopreservation can improve thawed sample quality. However, other authors have demonstrated that freezing the fresh sample and performing semen preparation after thawing gives better results in regard to total motile sperm count and motility. Regarding clinical results, it is well established that similar or even better reproductive outcomes are achieved using frozen testicular sperm in cases of azoospermia or anejaculation. Moreover, the use of frozen semen in cancer patients can help to achieve good fertilisation and pregnancy rates. Finally, the use of frozen sperm is not at all associated with worse post-natal development.

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Section: Dry Ice Freezingmentioning

confidence: 99%

Description and Outcomes of Current Clinical Techniques for Sperm Cryopreservation

Rios

Botella

2019

EMJ Repro Health

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“…39 Moreover, comparative studies of different cryopreservation protocols fail to find differences in sperm DNA integrity among the protocols studied. 20,22,[40][41][42][43][44] More remarkable is that most of these studies reported a high degree of DNA integrity, superior to 70%, after cryopreservation. 20,40,41,43,44 In contrast, some of these studies observed lower sperm DNA integrity, in the range of 15%, in thawed samples.…”

Section: Sperm Dna Integritymentioning

confidence: 99%

“…20,22,[40][41][42][43][44] More remarkable is that most of these studies reported a high degree of DNA integrity, superior to 70%, after cryopreservation. 20,40,41,43,44 In contrast, some of these studies observed lower sperm DNA integrity, in the range of 15%, in thawed samples. 22,42 However, it is relevant to note that the sperm DNA integrity prior to cryopreservation is not available in these cases.…”

Section: Sperm Dna Integritymentioning

confidence: 99%

Causes and Impact of Cryopreservation-Associated Damage on Different Parameters of Human Spermatozoa and its Clinical Impact

Rios

Botella

2019

EMJ Repro Health

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Sperm cryopreservation has been widely used for assisted reproductive technology (ART). Indications for sperm cryopreservation include donor insemination, cryopreservation prior to surgical infertility treatment, and malignancies to avoid additional surgery in couples undergoing repeated in vitro fertilisation (IVF) or intracytoplasmic sperm injection (ICSI) cycles. However, dramatic changes during cryopreservation have detrimental effects on the sperm membrane, resulting in a large increase in the percentage of poorly motile sperm or sperm with abnormal morphology. The negative effects related to rapid temperature decrease, such as osmotic injury, cellular dehydration, intracellular ice crystal formation, and oxidative stress can also damage the sperm in ways that affect reproductive outcome. This comprehensive review focusses on describing the detrimental effects of the cryopreservation process on sperm and aims to clarify that not all impaired sperm parameters have the same impact on the clinical practice of ART. Regarding the parameters studied, some of the biomarkers used for sperm maturity, hyaluronic acid binding capacity, or damaged DNA have limited clinical significance compared to other semen parameters which provide more useful information for clinical practice and are often dismissed, such as total motility or total motile sperm count (TMSC). In the authors’ experience, TMSC gives valuable quantitative information about the number of viable spermatozoa. Indeed, TMSC should be assessed specifically for groups of patients in which sample availability is limited.

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Green tea extract as a cryoprotectant additive to preserve the motility and DNA integrity of human spermatozoa

et al. 2021

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Effect of human sperm freezing-thawing process on vitrification and conventional freezing: evaluation of survival, motility, DNA oxidation and mitochondrial activity

Cited by 3 publications

References 0 publications

Description and Outcomes of Current Clinical Techniques for Sperm Cryopreservation

Description and Outcomes of Current Clinical Techniques for Sperm Cryopreservation

Causes and Impact of Cryopreservation-Associated Damage on Different Parameters of Human Spermatozoa and its Clinical Impact

Green tea extract as a cryoprotectant additive to preserve the motility and DNA integrity of human spermatozoa

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