Green tea extract as a cryoprotectant additive to preserve the motility and DNA integrity of human spermatozoa

Alqawasmeh, Odai; Zhao, Ming; Chan, C.; Leung, Maran Bw; Chow, Ki C; Agarwal, Nikunj; Mak, Jennifer Sm; Wang, Chi C; Pang, Chi Pui; Li, Tin C.; Chu, Wai Kit; Chan, David Yiu Leung

doi:10.4103/aja.aja_58_20

Cited by 10 publications

(4 citation statements)

References 31 publications

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“…The positive benefit of antioxidant therapy observed in this study is in keeping with the important role that ROS-induced lipid peroxidation plays in the loss of sperm function and DNA integrity associated with cryopreservation (Figures 4-6). The finding of improved DNA integrity, in particular, is in keeping with the results obtained by others with a variety of antioxidants, including ebselen [54], resveratrol [55], vitamin C [55], canthaxanthin [56], curcumin [57], L-carnitine [58], green tea extract [59], catalase [60], Tempol [61], alpha lipoic acid [62], and vitamin E [63]. Such results reinforce the conclusion that DNA damage in cryostored spermatozoa is primarily induced by oxidative stress, rather than by alternative mechanisms such as apoptosis [37].…”

Section: Discussionsupporting

confidence: 88%

Addition of Vitamin C Mitigates the Loss of Antioxidant Capacity, Vitality and DNA Integrity in Cryopreserved Human Semen Samples

Hungerford,

Bakos,

Aitken

2024

Antioxidants

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Cryopreservation of human spermatozoa is a necessity for males suffering from infertility who cannot produce fresh semen for insemination. However, current ART cryopreservation protocols are associated with losses of sperm motility, vitality and DNA integrity, which are thought to be linked to the induction of oxidative damage and the toxic properties of commercial cryoprotectants (CPAs). Preventing or mitigating these losses would be hugely beneficial to sperm survival during ART. Therefore, in this in vitro investigation, lipid peroxidation, production of reactive oxygen species, movement characteristics, antioxidant capacity, vitality, and DNA integrity were examined in semen samples both pre- and post-cryopreservation with CPA supplementation. The findings revealed a 50% reduction in antioxidant capacity with CPA addition, which was accompanied by significant increases in generation of reactive oxygen species and formation of lipid aldehydes. These changes were, in turn, correlated with reductions in sperm viability, motility and DNA integrity. Antioxidant supplementation generated bell-shaped dose-response curves with both resveratrol and vitamin C, emphasising the vulnerability of these cells to both oxidative and reductive stress. At the optimal dose, vitamin C was able to significantly enhance vitality and reduce DNA damage recorded in cryopreserved human spermatozoa. An improvement in sperm motility did not reach statistical significance, possibly because additional pathophysiological mechanisms limit the potential effectiveness of antioxidants in rescuing this aspect of sperm function. The vulnerability of human spermatozoa to reductive stress and the complex nature of sperm cryoinjury will present major challenges in creating the next generation of cryoprotective media.

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Section: Discussionsupporting

confidence: 88%

Addition of Vitamin C Mitigates the Loss of Antioxidant Capacity, Vitality and DNA Integrity in Cryopreserved Human Semen Samples

Hungerford,

Bakos,

Aitken

2024

Antioxidants

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“…Some observational studies have revealed that increased frequency and duration of green tea consumption signi cantly improved sperm concentration and motility [30][31][32]. However, only one study has examined the effects of green tea consumption on human sperm DNA integrity [33]. To investigate whether green tea extract (GTE) exerted protective bene ts on human sperm DNA, Alqawasmeh et al divided the sperm of the subjects into three groups (control, 300 µmol/l H 2 O 2 , and 300 µmol/l H 2 O 2 + 1.0 ng/ml GTE) and measured the DFI for each group.…”

Section: Discussionmentioning

confidence: 99%

“…To investigate whether green tea extract (GTE) exerted protective bene ts on human sperm DNA, Alqawasmeh et al divided the sperm of the subjects into three groups (control, 300 µmol/l H 2 O 2 , and 300 µmol/l H 2 O 2 + 1.0 ng/ml GTE) and measured the DFI for each group. They found that GTEtreated sperm exhibited a reduced DNA damage percentage as compared to the 300 µmol/l H 2 O 2 group (21.1% vs 25.2%, P < 0.05) [33]. Similarly, the protection of sperm DNA by GTE has also been demonstrated in rats, bucks, buffalo bulls, and goats [34][35][36][37].…”

Section: Discussionmentioning

confidence: 99%

Green tea is a protective factor that can preserve sperm DNA integrity: A retrospective study

Yang,

Geng,

Duan

et al. 2023

Preprint

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Background: Although sperm DNA fragmentation index (DFI) is an vital biomarker for sperm DNA integrity and male fertility, factors that influence DFI have not been fully elucidated, particularly for occupational exposures and lifestyle factors. Here, we sought to explore the effects of self-reported occupational exposures and lifestyle factors on sperm DNA integrity. Methods: The baseline characteristics, occupational exposures, and lifestyle factors of participants were collected immediately prior to or following semen evaluation through a self-report questionnaire. Participants were separated into three DFI-based groups: DFI<15%, 15%≤ DFI<30%, and 30%≤DFI. Initially, one-way ANOVAs and chi-square tests were employed to investigate the possible factors related to sperm DNA integrity. Then, we performed an ordered logistic regression analysis to detect factors independently associated with sperm DFI. To adjust for relevant confounders, we included factors with a P< 0.25 in univariate analyses as covariates. Results: Among 1,060 subjects who participated in this study, 826 met the icludion cretieria. Univariate analysis showed that age, infertility type, infertility duration, semen parameters (concentration, motility and morphology) and smoking were related to sperm DNA integrity (P<0.05). At ordered logistic regression analysis, compared with the group that drank green tea 5-7 times/week, the groups that drank green tea <1 time/week (OR=1.52, 95% CI=1.05-2.2, and p=0.027) and 1-4 times/week (OR=1.61, 95% CI=1.02-2.54, and p=0.042) exhibited significantly increased sperm DFI values. Of the traditional semen parameters measured, a positive correlation was noted between sperm DFI and semen volume (OR=1.18, 95% CI=1.07-1.31) and abnormal sperm morphology (OR=1.2, 95% CI=1.11-1.3), whereas it was negatively correlated with sperm motility (OR=0.94, 95% CI=0.93-0.95). Relative to men over 40 years of age, we detected a 65% (95% CI=0.2-0.62) and 58% (95% CI=0.26-0.7) decrease in sperm DFI values in the 20-30 and 30-40 age groups, respectively. Conclusions: These data suggest that green tea consumption is a protective factor associated with the preservation of sperm DNA integrity, which may offer new insights to guide the prevention and treatment of clinical male infertility.

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“…Giving green tea leaf extract could improve the quality and quantity of spermatozoa (Roychoudhury et al, 2017). Adding green tea extract to the extender could neutralize ROS and prevent DNA damage caused by ROS (Alqawasmeh et al, 2021). Until now, there has been no publication regarding the addition of green tea (Camellia sinensis) leaf extract (GTLE) in skim milk-goose egg yolk (SM-GEY) on the quality of Sapudi ram spermatozoa in 5°C storage.…”

Section: Introductionmentioning

confidence: 99%

Impact of green tea (Camellia sinensis) leaf extract in skim milk-goose egg yolk semen extender on the quality of Sapudi ram spermatozoa stored at 5°C

Ardina Sahra Miranda,

Tri Wahyu Suprayogi,

Budi Utomo

et al. 2023

Ovz

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Livestock production requires Sapudi rams, a breed native to Indonesia, to meet meat demand and food security. In artificial high-quality frozen semen is needed to spread Sapudi rams. To maximize the survival of spermatozoa during cryopreservation, semen should be stored in an extender. Green tea leaf extract (GTLE) and skim milk-goat egg yolk (SM-GEY) may be a good cryoprotectants due to their antioxidant properties. This study aimed to determine the effect of adding GTLE to the SM-GEY extender on the quality of Sapudi ram spermatozoa stored at 5°C. The fresh semen sample was divided into four different GTLE treatment groups, which each contained a 0.1 mL semen sample and a 25-mL extender of SM-GEY. Group T0: no GTLE added to SM-GEY; Groups T1, T2, and T3: 0.1 mL semen diluted in 25 mL SM-GEY with 0.05, 0.10, and 0.15 mg GTLE. Extended semen was then stored at 5°C, and its quality was evaluated daily for five days. The variables observed included spermatozoa motility, viability, and membrane integrity. Data were analyzed using one-way analysis of variance followed by Duncan's test using Statistical Program and Service Solution version 23. The result of this study was that adding 0.05 mg GTLE to 25 mL of SM-GEY extender significantly maintained the spermatozoa motility, viability, and plasma membrane integrity of Sapudi ram spermatozoa for three days at 5°C (p <0.05). Therefore, it could be concluded that adding 0.05 mg of GTLE to the SM-GEY extender preserved Sapudi ram spermatozoa's motility, viability, and membrane integrity for three days at 5°C.

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Green tea extract as a cryoprotectant additive to preserve the motility and DNA integrity of human spermatozoa

Cited by 10 publications

References 31 publications

Addition of Vitamin C Mitigates the Loss of Antioxidant Capacity, Vitality and DNA Integrity in Cryopreserved Human Semen Samples

Addition of Vitamin C Mitigates the Loss of Antioxidant Capacity, Vitality and DNA Integrity in Cryopreserved Human Semen Samples

Green tea is a protective factor that can preserve sperm DNA integrity: A retrospective study

Impact of green tea (Camellia sinensis) leaf extract in skim milk-goose egg yolk semen extender on the quality of Sapudi ram spermatozoa stored at 5°C

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