Effect of Huaier On the Proliferation of Mesangial Cells in Anti-Thy-1 Nephritis

Bai, Jiuxu; Wang, Geng; Mei, Yan; Wu, Lingling; Duan, Shuwei; Dong, Zheyi; Fu, Bo; Wang, Yong; Zhu, Fei; Cai, Guangyan; Feng, Zhe; Lin, Shupeng; Chen, Xiangmei

doi:10.1159/000480198

Cited by 22 publications

(30 citation statements)

References 40 publications

(37 reference statements)

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“…CASY cell analysis confirmed that Huaier effectively inhibited the proliferation of Hacat cells in a statistically significant time- and concentration-dependent manner (P <0.01). This is similar to many other studies, which have indicated that Hacat can inhibit the proliferation of tumor cells: previous experiments have confirmed that Huaier effectively inhibited the proliferation of melanoma, breast cancer, colon cancer, liver cancer, prostate cancer, stomach cancer, fibrosarcoma, lung cancer, and many other tumor cells [ 3 , 8 – 14 ]; in addition to tumor cells, previous studies have confirmed that Huaier inhibited the proliferation of some nontumor cells, such as mesangial cells and nodular sclerosis cells [ 15 , 16 ]. This study confirmed that Huaier effectively inhibited the excessive proliferation and accumulation of keratinocytes, which demonstrated an important link to the pathogenesis of psoriasis.…”

Section: Discussionmentioning

confidence: 99%

A Randomized, Double-Blind, Controlled Clinical Study on the Curative Effect of Huaier on Mild-to-Moderate Psoriasis and an Experimental Study on the Proliferation of Hacat Cells

Zhang

et al. 2018

BioMed Research International

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The antitumor effects of Huaier have been recently revealed. However, no research has been conducted on the effects of Huaier on keratinocyte proliferation and for the treatment of psoriasis. Hacat cells were treated with different concentrations of Huaier for different periods of times. The effects on cell proliferation and vitality and on the cell cycle were detected. Patients with mild-to-moderate psoriasis were randomized and divided into two groups in a double-blind manner. The experimental group was given sugar-free Yinxie granules and Huaiqihuang (HQH) granules, and the control group was given sugar-free Yinxie granules and placebo. After 4 weeks, various therapeutic indexes were compared. Huaier significantly inhibited Hacat cell proliferation, suppressed vitality, and blocked the cell cycle in the G1 phase compared with the control group (P < 0.01, respectively). After treatment for 4 weeks, the number of patients between the two groups that experienced a 50% reduction in the Psoriasis Area and Severity Index (PASI 50), PASI 75 and PASI 90, was significantly different (P <0.01). The body surface area (BSA) affected by psoriasis and static physician's global assessment (sPGA) was significantly reduced (P < 0.01); additionally, a significant improvement in the Dermatology Life Quality Index (DLQI) (P < 0.01) was observed. Huaier has shown promising effects in both clinical and experimental setting in this preliminary study and it might provide some benefit in the treatment of psoriasis vulgaris in the future.

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Section: Discussionmentioning

confidence: 99%

A Randomized, Double-Blind, Controlled Clinical Study on the Curative Effect of Huaier on Mild-to-Moderate Psoriasis and an Experimental Study on the Proliferation of Hacat Cells

Zhang

et al. 2018

BioMed Research International

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“…According to the research of Bai et al, PDGF-B is involved in the development of mesangial cells and overexpression of glomerular PDGF-B has an important effect on MsPGN disease. The research indicates that HQH can reduce the stimulation of PDGF-B, decrease the urinary protein, prolong G2 phase, and inhibit cell proliferation by regulating cyclin, so as to reduce the proliferation of anti-Thy-1 MsPGN [15]. As a result, HQH inhibits mesangial proliferation in MsPGN, promising a possible therapy in its clinical treatment.…”

Section: Mesangioproliferative Glomerulonephritis (Mspgn)mentioning

confidence: 96%

“…Studies of Li et al have shown that IgG is significantly higher after treatment with HQH than before treatment and CD3; CD4 are significantly higher than before treatment. Therefore, HQH can regulate the immune function and reduce infection in children with IgA nephropathy, indicating a quickly and effectively to treat it 15 . IgA can be improved by the antiproliferation and immunomodulatory properties of HQH.…”

Section: Mesangioproliferative Glomerulonephritis (Mspgn)mentioning

confidence: 99%

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The Effect of Chinese Traditional Medicine Huaiqihuang (HQH) on the Protection of Nephropathy

Zhang

Cheng

Zhou

et al. 2020

Oxidative Medicine and Cellular Longevity

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Kidney disease is one of the common diseases with high morbidity and high mortality, which brings a huge burden to the society and the patient’s family. The pathogenesis, treatment, and prognosis of kidney diseases are related to oxidative stress, inflammation, mitochondrial damage, and immune dysfunction. However, existing treatments always cause some damage to the kidneys. Kidney disease and immunosuppressant used together often lead to drug toxicity, patients with weakened immunity, organic rupture of the normal structure of the kidney, damage to the physiological function of the kidney, etc. Huaiqihuang is a kind of traditional Chinese medicine with a history of more than one thousand years. According to research, Robinia pseudoacacia can regulate the immune function by regulating oxidative stress, calcium inflow, and mitochondrial ATP. At the same time, it is also involved in regulating the ways of cell death, such as apoptosis, autophagy, ferroptosis, pyroptosis, and clockophagy, to reduce kidney damage, which has important clinical value. This article reviews the exact mechanism and clinical application of Huaiqihuang in different types of nephropathy. The aim is to provide new ideas for the treatment of clinical nephropathy.

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“…Currently, mesangial cells are recognized as the major cells to secrete ECM [ 5 ]. Therefore, inhibiting the proliferation of mesangial cells and the accumulation of ECM can be applied as a practical approach for treating or relieving DN [ 6 ]. However, the underlying mechanism is still unsatisfactory, and there is no effective therapeutic treatment drug.…”

Section: Introductionmentioning

confidence: 99%

Sauchinone Protects Renal Mesangial Cell Dysfunction against Angiotensin II by Improving Renal Fibrosis and Inflammation

Yoon

Lee

Kim

et al. 2020

IJMS

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Abnormal and excessive growth of mesangial cells is important in the pathophysiologic processes of diabetes-associated interstitial fibrosis and glomerulosclerosis, leading to diabetic nephropathy, which eventually turns into end-stage renal disease. Sauchinone, a biologically-active lignan isolated from aerial parts of Saururus chinensis, has anti-inflammatory and anti-viral activities effects on various cell types. However, there are no studies reporting the effects of sauchinone on diabetic nephropathy. The present study aims to investigate the role of sauchinone in mesangial cell proliferation and fibrosis induced by angiotensin II, as well as the underlying mechanisms of these processes. Human renal mesangial cells were induced by angiotensin II (AngII, 10 μM) in the presence or absence of sauchinone (0.1–1 μM) and incubated for 48 h. In this study, we found that AngII induced mesangial cell proliferation, while treatment with sauchinone inhibited the cell proliferation in a dose-dependent manner. Pre-treatment with sauchinone induced down-regulation of cyclins/CDKs and up-regulation of CDK inhibitor, p21, and p27kip1 expression. In addition, AngII-enhanced expression of fibrosis biomarkers such as fibronectin, collagen IV, and connective tissue growth factor (CTGF), which was markedly attenuated by sauchinone. Sauchinone also decreased AngII-induced TGF-β1 and Smad-2, Smad-3, and Smad-4 expression. This study further revealed that sauchinone ameliorated AngII-induced mesangial inflammation through disturbing activation of inflammatory factors, and NLRP3 inflammasome, which is composed of the NLRP3 protein, procaspase-1, and apoptosis-associated speck-like protein containing a CARD (ASC). Moreover, pretreatment of sauchinone inhibited NF-κB translocation and ROS production in AngII-exposed mesangial cells. These data suggest that sauchinone has a protective effect on renal proliferation, fibrosis and inflammation. Therefore, sauchinone might be a potential pharmacological agent in prevention of AngII-induced renal damage leading to diabetic nephropathy.

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Effect of Huaier On the Proliferation of Mesangial Cells in Anti-Thy-1 Nephritis

Cited by 22 publications

References 40 publications

A Randomized, Double-Blind, Controlled Clinical Study on the Curative Effect of Huaier on Mild-to-Moderate Psoriasis and an Experimental Study on the Proliferation of Hacat Cells

A Randomized, Double-Blind, Controlled Clinical Study on the Curative Effect of Huaier on Mild-to-Moderate Psoriasis and an Experimental Study on the Proliferation of Hacat Cells

The Effect of Chinese Traditional Medicine Huaiqihuang (HQH) on the Protection of Nephropathy

Sauchinone Protects Renal Mesangial Cell Dysfunction against Angiotensin II by Improving Renal Fibrosis and Inflammation

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