Effect of hormones on the variation of radiosensitivity in females as measured by induction of chromosomal aberrations.

Roberts, Charles; Morgan, GL; Danford, Natalie

doi:10.1289/ehp.97105s61467

Cited by 19 publications

(9 citation statements)

References 15 publications

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“…These reports clearly demonstrate that a variety of female hormones may play a role in modifying or enhancing DNA damage. 14 In this small study, the genotoxic effects of H. pylori prior to and following eradication therapy were analysed with SCE frequency analysis, a cytogenetic method. SCE frequency was found to be reduced significantly after eradication therapy in a group of gastritis patients infected with H. pylori.…”

Section: Discussionmentioning

confidence: 99%

The Role of Triple Therapy, Age, Gender and Smoking on the Genotoxic Effects of Helicobacter Pylori Infection

et al. 2002

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The role of age, gender and smoking on both the genotoxic effects of Helicobacter pylori and the efficacy of eradication therapy in a group of patients with gastritis was investigated. Gastritis was confirmed by endoscopy and biopsy, and the presence of H. pylori by urease testing. Pre- and post-treatment peripheral blood lymphocyte cultures were prepared from 17 patients and 25 metaphases per patients were analysed for sister chromatid exchange (SCE), a well-established technique for the evaluation of human exposure to toxic agents. Treatment with omeprazole, clarithromycin and amoxycillin triple therapy eradicated H. pylori in 94% of patients and significantly reduced the SCE frequency. Pre-treatment SCE frequency was found to be positively correlated with age. Female smokers tended to have higher post-treatment SCE frequencies than male smokers, and pre- and post-treatment SCE frequencies were higher in older males than in older females. Eradication therapy decreased the genotoxicity of H. pylori, but age in males and smoking in females may decrease treatment efficacy.

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Section: Discussionmentioning

confidence: 99%

The Role of Triple Therapy, Age, Gender and Smoking on the Genotoxic Effects of Helicobacter Pylori Infection

et al. 2002

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“…Biological dosimetry is further complicated by at risk populations, whether that is based on age, sex or genetic background. The current reference for dose limits and risk determination is based on the “Reference Man” by the International Commission on Radiological Protection (ICRP) (9), even though females, children, immunocompromised individuals and individuals with specific genetic mutations (i.e., ataxia telangiectasia mutated, Nijmegen breakage syndrome, Bloom Syndrome) have an increased risk for radiation-induced cancers and in some cases morbidity compared to this ICRP reference standard (9–12). …”

Section: Introductionmentioning

confidence: 99%

Development of a Metabolomic Radiation Signature in Urine from Patients Undergoing Total Body Irradiation

et al. 2014

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The emergence of the threat of radiological terrorism and other radiological incidents has led to the need for development of fast, accurate and noninvasive methods for detection of radiation exposure. The purpose of this study was to extend radiation metabolomic biomarker discovery to humans, as previous studies have focused on mice. Urine was collected from patients undergoing total body irradiation at Memorial Sloan-Kettering Cancer Center prior to hematopoietic stem cell transplantation at 4–6 h postirradiation (a single dose of 1.25 Gy) and 24 h (three fractions of 1.25 Gy each). Global metabolomic profiling was obtained through analysis with ultra performance liquid chromatography coupled to time-of-flight mass spectrometry (TOFMS). Prior to further analyses, each sample was normalized to its respective creatinine level. Statistical analysis was conducted by the nonparametric Kolmogorov-Smirnov test and the Fisher’s exact test and markers were validated against pure standards. Seven markers showed distinct differences between pre- and post-exposure samples. Of those, trimethyl-l-lysine and the carnitine conjugates acetylcarnitine, decanoylcarnitine and octanoylcarnitine play an important role in the transportation of fatty acids across mitochondria for subsequent fatty acid β-oxidation. The remaining metabolites, hypoxanthine, xanthine and uric acid are the final products of the purine catabolism pathway, and high levels of excretion have been associated with increased oxidative stress and radiation induced DNA damage. Further analysis revealed sex differences in the patterns of excretion of the markers, demonstrating that generation of a sex-specific metabolomic signature will be informative and can provide a quick and reliable assessment of individuals in a radiological scenario. This is the first radiation metabolomics study in human urine laying the foundation for the use of metabolomics in biodosimetry and providing confidence in biomarker identification based on the overlap between animal models and humans.

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“…C57BL/6 are known to allow the development of late side effects after irradiation unlike other genetic backgrounds that preferentially develop early effects. To overcome the impact of the ovarian cycle and thus avoid a known sex effect in radiation-induced carcinogenesis, experiments were performed on male animals 34 . Mice were housed in a continuously controlled environment, with enrichment adapted to their species.…”

Section: Methodsmentioning

confidence: 99%

Differential normal skin transcriptomic response in total body irradiated mice exposed to scattered versus scanned proton beams

Leduc

Chaouni

Pouzoulet

et al. 2021

Sci Rep

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Proton therapy allows to avoid excess radiation dose on normal tissues. However, there are some limitations. Indeed, passive delivery of proton beams results in an increase in the lateral dose upstream of the tumor and active scanning leads to strong differences in dose delivery. This study aims to assess possible differences in the transcriptomic response of skin in C57BL/6 mice after TBI irradiation by active or passive proton beams at the dose of 6 Gy compared to unirradiated mice. In that purpose, total RNA was extracted from skin samples 3 months after irradiation and RNA-Seq was performed. Results showed that active and passive delivery lead to completely different transcription profiles. Indeed, 140 and 167 genes were differentially expressed after active and passive scanning compared to unirradiated, respectively, with only one common gene corresponding to RIKEN cDNA 9930021J03. Moreover, protein–protein interactions performed by STRING analysis showed that 31 and 25 genes are functionally related after active and passive delivery, respectively, with no common gene between both types of proton delivery. Analysis showed that active scanning led to the regulation of genes involved in skin development which was not the case with passive delivery. Moreover, 14 ncRNA were differentially regulated after active scanning against none for passive delivery. Active scanning led to 49 potential mRNA-ncRNA pairs with one ncRNA mainly involved, Gm44383 which is a miRNA. The 43 genes potentially regulated by the miRNA Gm44393 confirmed an important role of active scanning on skin keratin pathway. Our results demonstrated that there are differences in skin gene expression still 3 months after proton irradiation versus unirradiated mouse skin. And strong differences do exist in late skin gene expression between scattered or scanned proton beams. Further investigations are strongly needed to understand this discrepancy and to improve treatments by proton therapy.

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Effect of hormones on the variation of radiosensitivity in females as measured by induction of chromosomal aberrations.

Cited by 19 publications

References 15 publications

The Role of Triple Therapy, Age, Gender and Smoking on the Genotoxic Effects of Helicobacter Pylori Infection

The Role of Triple Therapy, Age, Gender and Smoking on the Genotoxic Effects of Helicobacter Pylori Infection

Development of a Metabolomic Radiation Signature in Urine from Patients Undergoing Total Body Irradiation

Differential normal skin transcriptomic response in total body irradiated mice exposed to scattered versus scanned proton beams

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