Effect of honey on hepatotoxicity induced by antitubercular drugs in albino rats

Abstract

Background: Drug-induced hepatotoxicity is a potentially serious adverse effect of antituberculosis treatment (ATT) regimens containing isoniazid, rifampicin and pyrazinamide. Many in vitro and in vivo studies revealed that honey possess antioxidant property and hepotoprotective property but there is no systematic work available to test the effect of honey on antitubercular drugs induced hepatotoxicity in rats. Hence present study was carried out to explore the prophylactic and therapeutic effect of honey with its antioxidant activity against hepatotoxicity induced by antitubercular drugs (Isoniazid, Rifampicin and Pyrazinamide) in albino rats. Methods: Hepatotoxicity in rats treated with antitubercular drugs (Isoniazid, Rifampicin and Pyrazinamide) was studied by assessing parameters such as Serum alanine aminotransferase (ALT), Serum aspartate aminotransferase (AST), Serum total protein, Serum Malondialdehyde (MDA) and Serum Superoxide dismutase activity (SOD). The effect of Honey as co-administration and administration after establishment of hepatotoxicity on above parameter was investigated. These biochemical observations were supplemented by Histopathological examination of liver. Results: Honey significantly reversed changes in serum levels of AST, ALT, MDA, SOD, total protein and also histopathological changes produced by Antitubercular drugs. It was found that honey significantly prevented as well as reversed Antitubercular drugs induced hepatotoxicity and antioxidant activity. Conclusions: The results of present study show that honey has significant prophylactic and therapeutic value against antitubercular drugs induced hepatotoxicity. [Int J Basic Clin Pharmacol 2013; 2(2.000): 177-181

Hepatoprotective and Hepatocurative Effects of Nabk Honey in Penicillin-Induced Hepatic Toxicity

“…On the other hand, the present histological study showed that of Nabk honey reduced the cellular changes induced by penicillin treated groups in both hepatoprotective and hepatocurative studies, indicating that Nabk honey contributed to the protection against penicillin induced liver toxicity. Our observations are highly supported by the other studies which suggest that Nabk honey exert their protective and curative effects against some drugs 59,66-(doxorubicin, penicillin, streptomycin, ibuprofen, acetaminophen and paracetamol)-induced hepatotoxicity 13,32,[56][57][58][59]66 . On hypothesis to explain the beneficial effects of honey in ameliorating biochemical parameters and histological changes is that honey may contains flavonoids, ascorbic acid, tocopherols, catalase and phenolic compounds.…”

“…This might be the main reason for the restoration in the activities of the marker enzymes during administration of honey oxidative damage in a cell or tissue which occurs when the concentration of ROS (O 2 , H 2 O 2 and OH) generated exceeds the antioxidant capability of free radical scavenger 57 . Previous reports demonstrated that nabk honey has a protective role against many toxicants drugs 13,32,[56][57][58][59] . The present investigation clearly demonstrated that the injection of penicillin and antibiotics to guinea pigs have induced conspicuous alteration in the histological structure on the liver tissue in treated guinea pigs.…”

“…Numerous of biological and pharmacological properties of honey have been noted, including antibacterial, antifungal, anti-inflammatory, antioxidant, immunomodulatory, antiviral and anti carcinogenic properties [27][28][29][30] . The mentioned properties of the honey are mainly due to the presence of many chemical compositions such as polyphenolic composites including flavonoids, tannins, terpenoids and phenolic compounds, that are known to have a free-radical scavenging activity and reduce the levels of ROS 31,32 . This study was designed to investigate the protective and curative effect of nabk honey to reduce the side effects induced by penicillin on some biochemical parameters as well as liver tissue structure.…”

Effects of Kurdistan Honey on the Tongue of Chemotherapy Treated Albino Rats (Immunohistochemical Study)

“…In the distilled water treated group, a volume of distilled water equal to honey was given with gavage tube, while the honey-treated group was gavaged with honey at a dose of 2.5 g/kg two times daily, with a total of 5 g/kg/day [13]. A physiological saline (0.9% NaCl) in a similar dose of MTX (60 mg/Kg) interrupt the treatment at day four and administered intraperitoneally instead of MTX.…”

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“…[8] Drug-induced hepatotoxicity is mediated through oxidative stress, lipid peroxidation, and antioxidant alteration. [9] Therefore, it is proposed that the agents which reduce the lipid peroxidation level in tissue and elevate in tracellular antioxidant defenses may have hepatoprotective effect in people taking antitubercular drugs. To solve this problem, use of silymarin is suggested in clinical practice.…”