Effect of holder pasteurisation on total concentrations and iron-binding profiles of holo -lactoferrin used as fortifier in donor human milk

Menéndez, Sonia; Peixoto, Rafaella Regina Alves; Fernández-Colomer, Belén; Suarez-Rodríguez, Marta; Sanz‐Medel, Alfredo; Sánchez, Marı́a Luisa Fernández

doi:10.1016/j.idairyj.2019.104564

Cited by 6 publications

(1 citation statement)

References 28 publications

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“…For the typical minor serum proteins including IgG, IgA, IgM and LF, bovine milk has a concentration around 0.15–0.8, 0.05–0.14, 0.04–0.1 and 0.02–0.5 mg/mL, respectively, while human milk has a concentration around 0.03, 0.96, 0.02 and 1.5–2.0 mg/mL [ 4 ]. These bioactive proteins show antibacterial, anti-pathogenic, anticarcinogenic and anti-inflammatory properties [ 5 , 6 , 7 ]. Some minor serum proteins are generally thermal sensitive and susceptible to heat-induced denaturation, and hence inactivation [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

Transmission of Major and Minor Serum Proteins during Microfiltration of Skim Milk: Effects of Pore Diameters, Concentration Factors and Processing Stages

Liu

et al. 2021

Foods

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Effects of pore diameters (100, 50, and 20 nm), concentration factors (1–8) and processing stages (1–5) on the transmission of major serum proteins (β-lactoglobulin and α-lactalbumin) and minor serum proteins (immunoglobulin (Ig) G, IgA, IgM, lactoferrin (LF), lactoperoxidase (LPO), xanthine oxidase (XO)) during ceramic microfiltration (MF) of skim milk were studied. Holstein skim milk was microfiltered at a temperature of 50 °C, a transmembrane pressure of 110 kPa and a crossflow velocity of 6.7 m/s, using a tubular single stainless steel module that consisted of three ceramic tubes, each with 19 channels (3.5 mm inner diameter) and a length of 0.5 m. For MF with 100 nm and 50 nm pore diameters, the recovery yield of major serum proteins in permeate was 44.3% and 44.1%, while the recovery yield of minor serum proteins was slightly less by 0%–8% than 50 nm MF. MF with 20 nm pore diameters showed a markedly lower (by 12%–45%) recovery yield for both major and minor serum proteins, corresponding with its lower membrane flux. Flux sharply decreased with an increasing concentration factor (CF) up to four, and thereafter remained almost unchanged. Compared to the decrease (88%) of flux, the transmission of major and minor serum proteins was decreased by 4%–15% from CF = one to CF = eight. With increasing processing stages, the flux gradually increased, and the recovery yield of both major and minor proteins in the permeate gradually decreased and reached a considerably low value at stage five. After four stages of MF with 100 nm pore diameter and a CF of four for each stage, the cumulative recovery yield of major serum proteins, IgG, IgA, IgM, LF, LPO, and XO reached 95.7%, 90.8%, 68.5%, 34.1%, 15.3%, 39.1% and 81.2% respectively.

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