Effect of high-temperature on high-performance liquid chromatography column stability and performance under temperature-programmed conditions

Marin, Stephanie J.; Jones, Brian A.; Felix, W.D.; Clark, Jody

doi:10.1016/j.chroma.2003.10.092

Cited by 67 publications

(35 citation statements)

References 20 publications

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“…Marin et al [45] evaluated the stability of six commercially available columns under temperature-programmed high temperature LC conditions. They concluded that a Blaze C8 column can be used at maximum temperatures between 100 and 2008C with temperature programming without evidence of column degradation.…”

Section: Blaze 200 C-18 and Phidelity C-18mentioning

confidence: 99%

Temperature and pH‐stability of commercial stationary phases

Teutenberg¹,

Hollebekkers²,

Wiese

et al. 2009

J of Separation Science

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Original PaperTemperature and pH-stability of commercial stationary phasesIn this paper, the temperature and pH stability of silica-based RP stationary phases were investigated. Furthermore, nonsiliceous phases like a polymeric column based on polystyrene divinylbenzene and a polybutadiene coated zirconium dioxide column were also included. The columns were heated up to 1508C at dynamic conditions, which means that the eluent consisting of water and methanol (90:10, v/v) was continuously purged through the packed bed. After every 5 h, the columns were cooled down to room temperature and the efficiency was measured by injecting a test sample based on the Neue test. It could be shown that some stationary phases exhibited a very good temperature stability at the test conditions specified above.

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Section: Blaze 200 C-18 and Phidelity C-18mentioning

confidence: 99%

Temperature and pH‐stability of commercial stationary phases

Teutenberg¹,

Hollebekkers²,

Wiese

et al. 2009

J of Separation Science

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“…Literature on available stationary phases for high temperature and temperature-programmed LC is available [10,12,13,[15][16][17][18][19][20][21][22][23][24][25][26][27][28][29][30][31]. In 2004, a special issue of Journal of Chromatography A [32] was published on developments in stationary phases and retention, including phases for high temperature.…”

Section: Introductionmentioning

confidence: 99%

Elevated temperature and temperature programming in conventional liquid chromatography – fundamentals and applications

Vanhoenacker

Sandra²

2006

J of Separation Science

101

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Temperature, as a powerful variable in conventional LC is discussed from a fundamental point of view and illustrated with applications from the author's laboratory. Emphasis is given to the influence of temperature on speed, selectivity, efficiency, detectability, and mobile phase composition (green chromatography). The problems accompanying the use of elevated temperature and temperature programming in LC are reviewed and solutions are described. The available stationary phases for high temperature operation are summarized and a brief overview of recent applications reported in the literature is given.

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“…The gradient run induced a peak focusing effect coinciding with lower retention time, resulting in sharper peak shapes and less tailing. To measure this effect quantifiably, the average peak capacity was measured of the isothermal runs and the gradient run as described in literature [45][46][47]. A peak capacity of 55 was obtained for the gradient run (time interval of 20 min), while the peak capacities of the isothermal runs at 25, 40 and 60°C were 50, 54 and 38, respectively.…”

Section: Resultsmentioning

confidence: 99%