Effect of herbal hypoglycemic agents on oxidative stress and antioxidant status in diabetic rats

Mahdi, Abbas Ali; Chandra, Anu; Singh, Raj Kumar; Shukla, Sanjeev; Mishra, Lakshmi Chandra; Ahmad, Sohail

doi:10.1007/bf02867361

Cited by 57 publications

(31 citation statements)

References 16 publications

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“…[29,30] To elucidate the contribution of oxidative stress to the development of diabetes mellitus, we determined the effect of V. amygdalina on the level of lipid peroxidation product, malonialdehyde (MDA) which results from free radical damage to membrane components of the cells. [31] The mean level of MDA increased significantly (p ≤ 0.05) in the diabetic control compared to the normal group indicating increased oxidative stress in the diabetic rabbits.…”

Section: Discussionmentioning

confidence: 99%

Attenuation of Oxidative Damage in Alloxan Induced Diabetic Rabbits Following Administration of the Extract of the Leaves of Vernonia amygdalina.

Owolabi

Jaja

Olatunji

et al. 2011

Free Radicals and Antioxidants

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Introduction: Diabetes is a disease that constitutes multiple sources of free radicals, thus oxidative stress is expected to have a double impact. Since oxidative stress is mediated by hyperglycemia-induced generation of free radical, it is supposed that compounds with hypoglycemic and antioxidative properties would be useful antidiabetic agents. Our study investigated the use of V. amygdalina as a potential hypoglycemic and antioxidative agent by testing its effect on the antioxidant biomarkers and lipid peroxidation. Methods: Dried leaves of V. amgydalina were extracted for 48 hrs and freeze dried. Acute toxicity was investigated in 25 rats. Thirty six rabbits were divided into 6 groups: groups I -III were normal; diabetes was induced in groups (IV -VI). Groups I and IV were normal and diabetic controls respectively. The animals were treated with aqueous leaf extract of V. amygdalina. Blood samples were collected and used for the study. Results: The reduction in body weight in the diabetic groups was regained following administration of the extract of V. amgydalina. The extract is considered safe and had little or no effect on blood glucose, MDA and GSH levels of the normal rabbits. Extract significantly reduced glucose and MDA concentrations but increased GSH levels in the diabetic rabbits. Similarly, the extract had no effect on the activities of SOD, CAT and GPx in normal rabbits, however in diabetic rabbits, the enzymes activities increased dose-dependently. Conclusion: This finding provides basis for the use of V. amgydalina as potential antidiabetic antioxidant agent and may be useful for its hypoglycemic property. INTRODUCTIONWith oxidative stress linked to metabolic or vascular disorder, diabetes becomes the best disease for studying the consequences of oxidative stress and its treatment. Diabetes mellitus is a multifactorial disease characterized by hyperglycemia, [2,3] lipoprotein abnormalities, [4] raised basal metabolic rate, [5] defect in reactive oxygen species scavenging enzymes [6] and high oxidative stress induced by the generation of highly reactive free radicals which are also involved in pancreatic destruction. [2,7] Insufficient removal of these free radicals can lead to oxidative damage to membranes; enhance susceptibility to lipid peroxidation, enzyme inactivation and nucleic acid damage. [8,6] Since oxidative stress is mediated by hyperglycemiainduced generation of free radical, it became clear that lowering blood glucose as well as scavenging the free radicals may ameliorate the oxidative stress thus providing an effective strategy for reducing diabetic mellitus and its complications. Attenuation of Oxidative Damage in Alloxan Induced[9] Hence compounds with both hypoglycemic and antioxidative properties would be very useful antidiabetic agents. We therefore considered a known hypoglycemic plant, Vernonia amygdalina and its effect on some endogenous biomarkers of oxidative stress: superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT) and lipid peroxidation...

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Section: Discussionmentioning

confidence: 99%

Attenuation of Oxidative Damage in Alloxan Induced Diabetic Rabbits Following Administration of the Extract of the Leaves of Vernonia amygdalina.

Owolabi

Jaja

Olatunji

et al. 2011

Free Radicals and Antioxidants

View full text Add to dashboard Cite

show abstract

“…Fasting blood glucose levels were measured on day 3 and rats with blood glucose level greater than 9.7 mmol/l (175 mg/dl) were included in the study (Yadav et al, 2002). The standard anti-diabetic drug and test drugs were administered at 10 a.m. to the diabetic rats once daily at doses that were considered safe based on previous work (Sangraula et al, 2002;Mahdi et al, 2003).…”

Section: Experimental Designmentioning

confidence: 99%

Antioxidant and protective effect of latex of Calotropis procera against alloxan-induced diabetes in rats

Roy

Sehgal

Padhy

et al. 2005

Journal of Ethnopharmacology

117

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“…Diabetes mellitus, a disease of metabolic disorders, is associated with a number of chronic complications like nephropathy, neuropathy, retinopathy and cardiovascular diseases (Mahati, Chandra, & Singh, 2003). It still remains a major public health problem and prevention of diabetes still lies in the realm of future.…”

Section: Introductionmentioning

confidence: 99%

Antidiabetic effect of S-allylcysteine: Effect on Thyroid hormone and circulatory antioxidant system in experimental diabetic rats

Saravanan¹,

Ponmurugan²

2012

Journal of Diabetes and its Complications

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Effect of herbal hypoglycemic agents on oxidative stress and antioxidant status in diabetic rats

Cited by 57 publications

References 16 publications

Attenuation of Oxidative Damage in Alloxan Induced Diabetic Rabbits Following Administration of the Extract of the Leaves of Vernonia amygdalina.

Attenuation of Oxidative Damage in Alloxan Induced Diabetic Rabbits Following Administration of the Extract of the Leaves of Vernonia amygdalina.

Antioxidant and protective effect of latex of Calotropis procera against alloxan-induced diabetes in rats

Antidiabetic effect of S-allylcysteine: Effect on Thyroid hormone and circulatory antioxidant system in experimental diabetic rats

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