Effect of Heparin Surface Modification of Polymethylmethacrylate Intraocular Lenses on Signs of Postoperative Inflammation after Extracapsular Cataract Extraction

Borgioli, Dott Marco; Coster, Douglas J.; Fan, R. F. T.; Henderson, J. Gordon; Jacobi, K. W.; Kirkby, G R; Lai, Yoon Kee; Menezo, J.L.; Montard, M; Strobel, Jürgen; Wollensak, J.

doi:10.1016/s0161-6420(92)31816-0

Cited by 66 publications

(21 citation statements)

References 16 publications

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“…Three one-piece PMMA IOLs without surface modification (Pharmacia) and three heparin-surface-modified PMMA IOLs (Pharmacia) were tested. Two techniques for studying oil-IOL interaction were used: (I) immersion of IOLs in neat silicone oil, (2) immersion of IOLs in a suspension of emulsified sili cone oil droplets in saline solution. The emulsified drop lets were taken from a patient who had undergone oil removal for emulsification.…”

Section: Methodsmentioning

confidence: 99%

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The adherence of silicone oil to standard and heparin-coated PMMA intraocular lenses

Batterbury

Wong

Williams

et al. 1994

Eye

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Section: Methodsmentioning

confidence: 99%

“…It has been shown that inflammatory cells are less likely to adhere to these surface-modified IOLs. 2 The bound heparin renders the PMMA surface more hydrophilic,1 which will influence the interaction between the material surface and the silicone oil, such that the oil should spread less and therefore be less strongly adherent to the IOL.…”

mentioning

confidence: 99%

The adherence of silicone oil to standard and heparin-coated PMMA intraocular lenses

Batterbury

Wong

Williams

et al. 1994

Eye

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“…In vitro studies of heparin surface-modified (HSM) IOLs showed reduced fibroblast, granulocyte and macrophage activation, reduced complement activation as well as re duced adherence of endothelial cells and thrombocytes when compared to regular PMMA IOLs [9][10][11]. Following implantation in animal and human eyes, HSM IOLs showed less synechiae, cells, fibrin, pigment and precipitates on IOL surfaces [12][13][14].…”

Section: Fig 1 Heparin (Anionic Polyclectrolytc)mentioning

confidence: 99%

A New Method for Direct Detection of Heparin on Surface-Modified Intraocular Lenses

Dick¹,

Eisenmann³

et al. 1997

Ophthalmologica

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Background: Examination of surface-bound heparin on synthetic polymers can only be performed by few staining methods. These methods are limited by an only approximate detection of heparin visible at high magnification. Other methods only measure heparin quantitatively (per square dimension) and are rather sensitive to artifacts. Due to its homogeneous staining pattern, the modified toluidine blue staining technique, using a non-protein-based substance, allows examination and analysis of the homogeneity of the monomolecular heparin layer even under critical conditions like scanning electron microscopy. Materials and Methods: For critical examination of the heparin layer, this method was used for 5 sterile heparin surface-modified (HSM) monofocal (Pharmacia, 809C, 5 mm optical zone), 2 multifocal PMMA (Pharmacia, 81 IE, 6 mm optical zone) and 1 standard PMMA intraocular lenses (IOLs; Pharmacia, 809P, 5 mm optical zone). A special mixture containing a sodium borate buffer was used to avoid cross-reactions of toluidine with phenylimines, which permit a covalent surface linkage of heparin to synthetic polymeric materials via reductive amination. This resulted in less coarse-grained complex agglutination. After light and spectral microscopy of the surface of stained IOLs, scanning electron microscopy was performed to investigate the reliability and validity of this modified staining method. Results: All HSM IOLs showed a homogeneous heparin structure and coating, which could be demonstrated even under critical photographic circumstances. Conclusions: The modification of the original toluidine blue staining method introduced by Jaques in 1943 is a reliable and reproducible technique for the detailed in vitro analysis of surface-bound heparin with a low artifact rate. Because of the detailed detection of heparin on polymeric surfaces, this staining method is recommended for special problems, e.g. in cataract surgery.

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“…It can be dissolved in liquid fluorocarbons, 3 which allows its use as a coating of PMMA IOLs rendering them highly hydrophobic. Other techniques can be used to modify the surface of PMMA IOLs, rendering them highly hydrophilic [4][5][6][7] or highly hydrophobic. [8][9][10] The highly hydrophilic heparin-surface-modified PMMA IOLs have anti-adhesive properties that are correlated with increased biocompatibility in vivo.…”

Section: Introductionmentioning

confidence: 99%

“…[8][9][10] The highly hydrophilic heparin-surface-modified PMMA IOLs have anti-adhesive properties that are correlated with increased biocompatibility in vivo. [5][6][7] In a previous study, we used an organ culture method to evaluate cell adhesion, proliferation, and migration on Teflon-coated IOLs (Werner L, Legeais JM, Nagel MD, Renard G. Evaluation of Teflon-coated intraocular lenses in an organ culture method. J Biomed Mater Res, to appear).…”

Section: Introductionmentioning

confidence: 99%

Neutral red assay of the cytotoxicity of fluorocarbon-coated polymethylmethacrylate intraocular lenses in vitro

Werner

Legeais

Nagel

et al. 1999

J. Biomed. Mater. Res.

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Polymethylmethacrylate (PMMA) intraocular lenses (IOLs) were coated with Teflon AF, an amorphous, transparent Teflon, to render them highly hydrophobic. Teflon-coated PMMA IOLs were immersed in culture medium for 30 days at 37 degrees C. Four concentrations of the IOL leachables, 2 concentrations of a toxic control (phenol), and complete liquid culture medium (nontoxic control) were incubated for 24 h in a 96-well plate containing confluent L-929 fibroblasts. The cytotoxic effect of each solution on the fibroblasts was quantitatively assessed by measuring the uptake of neutral red by the viable cells. After the extraction of the neutral red using 1% acetic acid-50% ethanol, the optical densities were measured with a microplate reader at 550 nm. Scanning electron microscopy (SEM) and X-ray photoelectron spectroscopy (XPS) were used to analyze the surfaces of the IOLs. Only the optical densities in the wells containing fibroblasts that had been in contact with the phenol solutions were significantly lower than those in the wells incubated with the nontoxic control solution (p < 0.01). There were no signs of surface alteration by SEM, apart from some crystals on the IOLs. The crystals were composed of Na and Cl, as demonstrated by XPS. Aqueous extractables from the Teflon-coated IOLs produced no cytotoxic effects in the neutral red assay used.

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Effect of Heparin Surface Modification of Polymethylmethacrylate Intraocular Lenses on Signs of Postoperative Inflammation after Extracapsular Cataract Extraction

Cited by 66 publications

References 16 publications

The adherence of silicone oil to standard and heparin-coated PMMA intraocular lenses

The adherence of silicone oil to standard and heparin-coated PMMA intraocular lenses

A New Method for Direct Detection of Heparin on Surface-Modified Intraocular Lenses

Neutral red assay of the cytotoxicity of fluorocarbon-coated polymethylmethacrylate intraocular lenses in vitro

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