Effect of Heavy Metal Contamination in the Environment on Antioxidant Function in Wumeng Semi-fine Wool Sheep in Southwest China

Self Cite

The purpose of this research is to better understand the effect of different copper (Cu) levels of feed on the antioxidant capacity in the native sheep, solve the adverse effects of local seasonal Cu deficiency on sheep, and provide a reasonable reference for Cu supplementation. 10 healthy native sheep were selected as the control group (C group). 40 Cu-deficient native sheep were selected as tested group, and divided randomly into 4 groups (n = 10), supplied by CuSO 4 (0, 20, 40, 60 mg/kg for group I, group II, group III and group IV, respectively). The results showed that the level of Cu, hemoglobin (Hb), packed-cell volume (PCV), albumin (ALB), the activities of ceruloplasmin (CP), Cu/Znsuperoxide dismutase (Cu/Zn-SOD) and catalase (CAT) in group I were significantly lower than those in C group, group II, III, IV (P<0.05), but malondialdehyde (MDA) concentration was higher. The activities of these antioxidant enzymes and MDA concentration had no significant difference between C group, group II, III, IV (P>0.05), but group III had the highest antioxidant enzymes activity and the lowest MDA concentration. There was no significant difference in manganese (Mn), zinc (Zn), iron (Fe), molybdenum (Mo), selenium (Se), red blood cell count (RBC), white blood cell count (WBC), blood urea nitrogen (BUN), alanine transaminase (ALT), alkaline phosphatase (ALP), aspartate aminotransferase (GOT), total cholesterol (TC), manganese-superoxide dismutase (Mn-SOD) between all groups (P>0.05). Conclusion: The function of antioxidant system of the native sheep in Weining County have seriously reduced. Added CuSO 4 to the diet increase the activity of antioxidant enzymes and improve the health parameters, but them did not increase linearly. Added 40 mg/kg CuSO 4 , the activity of antioxidant enzymes was the highest and the content of MDA was the lowest. Therefore, it is recommended to add 40 mg/kg CuSO 4 to diet of the native sheep in Weining County.

Section: Introductionmentioning

confidence: 99%

Effect of Different Copper Levels in Feed on Antioxidant Capacity in Stocking the Native Sheep in the Wumeng Prairie

Wu¹,

Xiong²,

Jian³

2021

Self Cite

“…Different lowercase letters in the same trade indicates significant difference at P<0.01 level. sheep [8,22,23]. In addition, metallothionein (MT) and metal binding protein (MBP) can be regulated to prevent or reduce its toxicity.…”

Section: Effects Of S Fertilization On Antioxidant Capacitymentioning

confidence: 99%

“…Hydrogen peroxide is degraded and removed by CAT and GSH-Px, which keeps the organism in a good immune state [27]. Cu is also a component of SOD catalytic center in organism, which is related to SOD catalytic activity [22]. When the Cu content in the body is extremely excessive, it shows certain toxicity.…”

Section: Effects Of S Fertilization On Antioxidant Capacitymentioning

confidence: 99%

“…GSH-Px and CAT can clear H 2 O 2 in the body, so as to protect cells from H 2 O 2 damage [28]. As the comprehensive indicator for evaluating levels of antioxidant enzymes and the non-enzymatic system in animal organisms, T-AOC can reflect the compensatory capacity to external stimuli and the metabolism capacity of free radicals [22,37]. Decreased level of T-AOC cannot keep antioxidant system active, leading to the abundance of lipid peroxides and free radicals.…”

Section: Effects Of S Fertilization On Antioxidant Capacitymentioning

confidence: 99%

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Effects of Sulfur Fertilization on Antioxidant Capacity of Wumeng Semi-Fine Wool Sheep in the Wumeng Prairie

Li¹,

Wang²,

Shen³

2021

Self Cite

To assess the impact of sulfur(S) fertilization on antioxidant capacity of grazing Wumeng semifine wool sheep in copper(Cu) polluted meadow, and explore the control methods of Cu pollution in natural pasture, fertilizer treatment and grazing experiments were carried out in Weining County of the Wumeng Prairie, Guizhou Province, Southwest China. 24 ha Cu-polluted meadows were fenced, and randomly divided into four groups (3 replications/group, 2 ha/replication): 1) control group, no fertilizer; 2) the tested groups, applied ammonium sulfate [(NH4) 2 SO 4 ] (300, 400, and 500 kg/ha for group I, group II, and group III, respectively). A total of 72 healthy Wumeng semi-fine wool sheep (aged one year, 32.8±1.3 kg) were used in this study. All animals were randomly divided into 4 groups (3 replications/group, 6 sheep/replication) and assigned to the tested pastures. The grazing experiment lasted for 60 days. The results showed that the contents of N, Mn, Zn, and S in herbage in fertilized pastures were markedly higher than those in control group (P<0.01). The contents of Cu, Fe, and Se in herbage in fertilized pastures were significantly lower than those in control group (P<0.01). There were no extreme differences among the fertilized pastures (P>0.05). The contents of Mn, Zn, and S in serum and liver in the tested sheep were significantly higher than those in control animals (P<0.01). The contents of Cu, Fe, and Se in serum and liver in the tested sheep were extremely lower than those in control animals (P<0.01). The levels of Hb, RBC, and PCV in blood in the tested sheep, and the activities of serum SOD, GSH-Px, T-AOC, CAT, and Cp in the tested sheep were extremely higher than those in control sheep (P<0.01). The contents of serum MDA in the tested sheep were significantly lower than those in control animals (P<0.01). There were no great differences among the tested groups in physiological and biochemical parameters (P>0.05). The current results indicated that fertilization of

“…Cu element may be reversibly removed, and it is required for activity [22,23]. Cu deficiency often leads to low Cp content in blood, and decreases absorption of Fe [24,25]. Therefore, the levels of T-AOC, CAT, and SOD in the Tibetan sheep from fertilized pastures were extremely decreased, and the contents of MDA were considerably increased.…”

Section: Influences Of S Fertilization On Antioxidantmentioning

confidence: 99%

Effects of Sulphur Fertilizer on Copper Metabolism in Grazing Tibetan Sheep in Fertilized Pasture

Zhai¹,

Zhao²,

Shen³

2021

Self Cite

To explore the impact of sulfur (S) fertilization on copper (Cu) metabolism in grazing Tibetan sheep, fertilizing and grazing experiments were conducted in the Southwest Grand Grassland, China. The tested pasture was randomly divided into four parts by the fencing device (2 hm 2 /part). The pasture received ammonium sulfate [(NH 4 ) 2 SO 4 ] treatment in summer in 2020 (0, 300, 400, and 500 kg/hm 2 for part I, part II, part III, and part IV, respectively). 24 Tibetan sheep (one year old) were randomly divided into four groups, and assigned to the selected pastures (part I, part II, part III, and part IV for group I, group II, group III, and group IV, respectively). The grazing experiments lasted for 90 days. The results showed that the contents of nitrogen (N) and S in fertilized forage were markedly higher than those in unfertilized grass (P<0.01), but there was no significant difference among fertilized forages. The contents of Cu in blood and liver in the Tibetan sheep from fertilized pasture were extremely lower than those in animals from unfertilized pasture (P<0.01). The contents of Hb and PCV in the tested sheep from fertilized pasture were extremely higher than those in sheep from unfertilized pasture. The activities of serum SOD, GSH-Px, T-AOC, Cp, and CAT in sheep from fertilized pasture were extremely lower than those in sheep from no fertilized pasture (P<0.01), but there were no obvious differences among the Tibetan sheep from fertilized pasture (P>0.05). The contents of MDA in the Tibetan sheep from fertilized pasture were significantly higher than those in animals from no fertilized pasture (P<0.01). There was no obvious difference among the Tibetan sheep from fertilized pasture (P>0.05). Therefore, (NH 4 ) 2 SO 4 fertilization not only greatly influenced the contents of S and N