Effect of heat treatment on H2O2/HCl etched pure titanium dental implant: An in vitro study

Abstract

SummaryBackgroundSurface chemistry of dental implant plays an important role in osseointegration. Heat treatment might alter surface chemistry and result in different biological response. The aim of this study was to investigate the roles of heat treatment of H2O2/HCl-treated Ti implants in cell attachment, proliferation and osteoblastic differentiation.Material/MethodsSandblasted, dual acid-etched and H2O2/HCl heat-treated discs were set as the control group and sandblasted, dual acid-etched H2O2/HCl-treated discs were the test group. Both groups’ discs were sent for surface characterization. MC3T3-E1 cells were seeded on these 2 groups’ discs for 3 hours to 14 days, and then cell attachment, cell proliferation and cell differentiation were evaluated.ResultsScanning electron microscope analysis revealed that the titanium discs in the 2 groups shared the same surface topography, while x-ray diffraction examination showed an anatase layer in the control group and titanium hydride diffractions in the test group. The cell attachment of the test group was equivalent to that of the control group. Cell proliferation was slightly stimulated at all time points in the control group, but the alkaline phosphatase (ALP) activity and osteocalcin (OC) production increased significantly in the test group compared with those in the control group at every time point investigated (p<0.05 or p<0.01). Moreover, the osteoblastic differentiation-related genes AKP-2, osteopontin (OPN) and OC were greatly up-regulated in the test group (p<0.05 or p<0.01).ConclusionsThe results implied that surface chemistry played an important role in cell response, and H2O2/HCl etched titanium surface without subsequent heat treatment might improve osseointegration response.

Surface-treated commercially pure titanium for biomedical applications: Electrochemical, structural, mechanical and chemical characterizations

“…Several authors [27][28][29]52] have investigated the effect of HCl/H 2 O 2 treatment on the peri-implant bone formation. The osteoblastic response was improved for HCl/H 2 O 2 etched surface when compared to sandblasted one [52].…”

“…Several authors [27][28][29]52] have investigated the effect of HCl/H 2 O 2 treatment on the peri-implant bone formation. The osteoblastic response was improved for HCl/H 2 O 2 etched surface when compared to sandblasted one [52]. Contradicting our results, Faverani et al [53] observed that double acid-etched reduced the corrosion stability of Ti\ \6Al\ \4V alloy in SBF containing different concentrations of dextrose and lipopolysaccharide versus machined surface.…”

Effect of chemical and thermochemical treatments on the surface properties of commercially pure titanium

“…The modification of the chemistry and phase compositions of the surface has been tested with success to improve both the biocompatibility and bioactivity of the titanium [4,5]. In order to achieve this purpose, coatings of calcium phosphates, oxides, hydroxides, and titanates have been used [6][7][8][9]. Also, the modification of the surface roughness and (or) topography has been successfully employed to enhance bioactivity on titanium and its alloys [7,10].…”

Biological characterization of implant surfaces - in vitro study

“…These properties determine which proteins will be adsorbed, the amount and the orientation they will have on the surface, thereby influencing mechanisms such as recruitment, adhesion, proliferation and cellular differentiation 6,18 . Thus, it is understood that the surface treatment of titanium also acts indirectly in osteoblast adhesion from the interaction with proteins that will mediate cellular adhesion and differentiation with contribution of proteins from the extracellular matrix, membrane and cytoskeleton 19,20 .…”