Effect of Heat Stress on the Expression of GABA Receptor mRNA in the HPG Axis of Wenchang Chickens

Lj, Xie; Xie, XZ; Yw, Zhou; Chen, Liang; Yy, Jiang; Chen, Z

doi:10.1590/1806-9061-2015-0065

Cited by 4 publications

(6 citation statements)

References 14 publications

(17 reference statements)

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“…A characteristic of broiler chickens reared under high temperature stress is a rapid decline of weight gain after the early period. According to multiple reports on the rearing of broiler chickens and turkeys, body weight, feed intake, and feed conversion ratio decreased in high temperature environments compared to a general environment [ 18 , 19 ], and the present study also confirmed these phenomena. The present study identified the effects of the addition of a single supplement, GABA (GB50, GB150).…”

Section: Discussionsupporting

confidence: 87%

Effects of gamma aminobutyric acid on performance, blood cell of broiler subjected to multi-stress environments

Park¹,

Oh²,

Joo³

et al. 2023

Anim Biosci

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Objective: Stress factors such as high temperatures, overcrowding, and diurnal temperature range exert profound negative effects on weight gain and productivity of broiler chickens. The potential of gamma aminobutyric acid (GABA) as an excitatory neurotransmitter was evaluated under various stress conditions in this study.Methods: The experiment was conducted under four different environmental conditions: normal, high temperature, overcrowded, and in an overcrowded-diurnal temperature range. The experimental groups were divided into (–) control group without stress, (+) control group with stress, and G50 group (GABA 50 mg/kg) with stress. Weight gain, feed intake, and feed conversion ratio were measured, and stress reduction was evaluated through hematologic analysis.Results: The effects of GABA on broilers in four experimental treatments were evaluated. GABA treated responded to environmental stress and improved productivity in all the experimental treatments. The magnitude of stress observed was highest at high temperature, followed by the overcrowded environment, and was least for the overcrowded-diurnal temperature range.Conclusion: Various stress factors in livestock rearing environment can reduce productivity and increase disease incidence and mortality rate. To address these challenges, GABA, an inhibitory neurotransmitter, was shown to reduce stress caused due to various environmental conditions and improve productivity.

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Section: Discussionsupporting

confidence: 87%

Effects of gamma aminobutyric acid on performance, blood cell of broiler subjected to multi-stress environments

Park¹,

Oh²,

Joo³

et al. 2023

Anim Biosci

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“…However, the mRNA expression of Gad1 was significantly reduced in fish supplied with GABA in the diet which might ultimately balance the GABA levels in the CNS of juvenile olive flounder at the high water temperature and relieve the temperature stress. Likewise, Xie et al [ 46 ] reported the positive effects of supplemental GABA in terms of increased mRNA expression of GABAergic receptors such as GABA A and GABA B in the hypothalamic–pituitary–gonadal (HPG) axis of Wenchang chickens. However, these researchers also reported that the GABA A and GABA B receptors showed fluctuations at mRNA levels and variability in the tissues of the HPG over 1–6 weeks of heat stress in chickens.…”

Section: Discussionmentioning

confidence: 99%

Interactive Effect of Dietary Gamma-Aminobutyric Acid (GABA) and Water Temperature on Growth Performance, Blood Plasma Indices, Heat Shock Proteins and GABAergic Gene Expression in Juvenile Olive Flounder Paralichthys olivaceus

et al. 2023

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Gamma-aminobutyric acid (GABA) is an important inhibitory neurotransmitter in the central nervous system of living organisms and has the ability to reduce the magnitude of stress in humans and animals. In this study, we evaluated the supplemental effects of GABA on normal and high water temperature based on growth, blood plasma composition as well as heat shock proteins and GABA-related gene expression in juvenile olive flounder. For this, a 2 × 2 factorial design of experiment was employed to investigate the dietary effects of GABA at 0 mg/kg of diet (GABA0 diet) and 200 mg/kg of diet (GABA200 diet) in water temperatures of 20 ± 1 °C (normal temperature) and 27 ± 1 °C (high temperature) for 28 days. A total of 180 fish with an average initial weight of 40.1 ± 0.4 g (mean ± SD) were distributed into 12 tanks, of which, each tank contained 15 fish based on the 4 dietary treatment groups in triplicate. At the end of the feeding trial, the results demonstrated that both temperature and GABA had significant effects on the growth performance of the fish. However, fish fed the GABA200 diet had a significantly higher final body weight, weight gain and specific growth rate as well as a significantly lower feed conversion ratio than the fish fed the GABA0 diet at the high water temperature. A significant interactive effect of water temperature and GABA was observed on the growth performance of olive flounder based on the two-way analysis of variance. The plasma GABA levels in fish were increased in a dose-dependent manner at normal or high water temperatures, whereas cortisol and glucose levels were decreased in fish fed GABA-supplemented diets under temperature stress. The GABA-related mRNA expression in the brains of the fish such as GABA type A receptor-associated protein (Gabarap), GABA type B receptor 1 (Gabbr1) and glutamate decarboxylase 1 (Gad1) were not significantly affected by GABA-supplemented diets under normal or temperature stressed conditions. On the other hand, the mRNA expression of heat shock proteins (hsp) in the livers of the fish, such as hsp70 and hsp90, were unchanged in fish fed the GABA diets compared to the control diet at the high water temperature. Collectively, the present study showed that dietary supplementation with GABA could enhance growth performance, and improve the feed utilization, plasma biochemical parameters and heat shock proteins and GABA-related gene expression under the stress of high water temperatures in juvenile olive flounder.

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“…In addition, functional compounds like GABA (a non-protein amino acid) functions as neurotransmitter inhibitor and exhibit hypotensivity [7]. GABA is one of the most important functional components in fermented foods due to its physiological functions such as neurotransmission and antihypertensive activities [8], and anti-heat stress for broilers [9]. Nonetheless not much is known about GABA-rich fermented foods that can be used as feed additives.…”

Section: Introductionmentioning

confidence: 99%

γ-Aminobutyric acid production by selected lactic acid bacteria isolate of an Indonesian indigenous fermented buffalo milk (dadih) origin

Harnentis¹,

Nurmiati²,

Marlida³

et al. 2019

Vet World

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Aim: This study aimed at optimizing γ-aminobutyric acid (GABA) production using lactic acid bacteria (LAB) of an Indonesian indigenous fermented buffalo milk (dadih) origin. This study utilized LAB previously cultured from dadih that has the ability to produce GABA. Materials and Methods: The study started with the identification of selected LAB by 16S rRNA, followed by optimization of GABA production by culture conditions using different initial pH, temperature, glutamate concentration, incubation time, carbon, and nitrogen sources. 16S rRNA polymerase chain reaction and analysis by phylogenetic were used to identify Lactobacillus plantarum (coded as N5) responsible for the production of GABA. Results: GABA production by high-performance liquid chromatography was highest at pH of 5.5, temperature of 36°C, glutamate concentration of 500 mM, and incubation time of 84 h. Peptone and glucose served as the nitrogen and carbon sources, respectively, whereas GABA was produced at optimum fermentation condition of 211.169 mM. Conclusion: Production of GABA by L. plantarum N5 was influenced by initial pH of 5.5, glutamic acid concentration, nitrogen source, glucose as carbon source, and incubation temperature and time. Keywords: fermented buffalo milk, Indonesian indigenous product, lactic acid bacteria, γ-aminobutyric acid.

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Effect of Heat Stress on the Expression of GABA Receptor mRNA in the HPG Axis of Wenchang Chickens

Cited by 4 publications

References 14 publications

Effects of gamma aminobutyric acid on performance, blood cell of broiler subjected to multi-stress environments

Effects of gamma aminobutyric acid on performance, blood cell of broiler subjected to multi-stress environments

Interactive Effect of Dietary Gamma-Aminobutyric Acid (GABA) and Water Temperature on Growth Performance, Blood Plasma Indices, Heat Shock Proteins and GABAergic Gene Expression in Juvenile Olive Flounder Paralichthys olivaceus

γ-Aminobutyric acid production by selected lactic acid bacteria isolate of an Indonesian indigenous fermented buffalo milk (dadih) origin

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