“…Studies have reported that heat stress begins when the temperature humidity index value exceeds 65 and mortality rates increase when it exceeds 80 (Vitali et al, 2009;Collier et al, 2011). Many studies have been conducted to show that the heat stress negatively affects milk yield (West, 1999;West et al, 2003;Brouček et al, 2009;Baumgard et al, 2012;Brown et al, 2015;Al Reyad et al, 2016;Trajchev et al, 2016;Zhu et al, 2016) and milk fat rate (Arieli et al, 2004;Rejeb et al, 2012;Brouček et al, 2009;Ghavi Hossein-Zadeh, 2013). However, studies reporting that heat stress negatively affects the reproductive characteristics of animals have also been conducted (Evans et al, 2010;Khodaei-Motlagh et al, 2011;El-Wishy, 2013).This study aimed to investigate the effect of heat stress on milk yield, milk fat ratio and body temperature in Holstein-Friesian dairy cattle.…”