Effect of Headspace and Trapped Volatile Organic Compounds (VOCs) of the Chinese Caterpillar Mushroom, Ophiocordyceps sinensis (Ascomycetes), against Soil-Borne Plant Pathogens

Sangeetha, C.; Krishnamoorthy, A. S.; Kumar, Niranjan; Pravin, I. Arumuka

doi:10.1615/intjmedmushrooms.2018027311

Cited by 7 publications

(7 citation statements)

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“…This method has been used to validate the VOCs emitted by fungi, bacteria, and yeasts by several workers [7,9,28] . The trapping of volatiles by air entrainment technique was followed as suggested by Sangeetha et al, (2018) citratus read through MS spectral library strongly supported the earlier results of authors [29,23,20] who have documented matching VOCs profile with various species of M. spicata and C. citratus. The FT-IR was performed to identify the functional group of organic components present in the leaves of M. spicata and C. citratus based on peaks shown in the region of IR radiation.…”

Section: Antifungal Activity Of Vocsupporting

confidence: 80%

Antifungal volatiles from medicinal herbs suppress Fusarium oxysporum f. sp. lycopersici

T¹,

As²,

Nakkeeran³

et al. 2021

J. Entomol. Zool. Stud.

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Medicinal herbs are the potential sources for antimicrobial compounds against soil-borne pathogens. The present investigation focuses on assessing the efficacy of antifungal activity of volatile organic compounds (VOCs) produced by seven different medicinal herbs in the suppression of F. oxysporum f.sp. lycopercisi, the causal agent of wilt disease in tomato plants. Among the seven samples tested invitro, the blend of VOCs emitted from mint (Mentha spicata) leaves inhibited the mycelial growth of F. o. f.sp. lycopercisi up to 41.88% followed by lemongrass (39.44%) and nochi leaves (34.66%). The important VOCs emitted by mint and lemongrass samples were identified through Headspace -Gas chromatography coupled with Mass Spectrophotometry (HS-GCMS) which revealed the presence of around 25 different compounds with varied functional groups. Among them -(-) carvone obtained from mint leaves had the highest relative abundance (2.08% peak area at 10.25 RT). In addition, geraniol and citronellol were also present with a peak area percentage of 15.85 at 10.37 RT and 5.27 at 9.97 RT, respectively. Pure compounds of carvone, geraniol and citronellol (Sigma Aldrich) along with plant samples were also tested in vitro for comparison on the inhibition of mycelial growth of F. oxysporum f. sp. lycopercisi. Among the samples, citronellol was found to be the most effective and suppressed the wilt pathogen up to 100% at 500 ppm, followed by carvone (up to 90% inhibition) in the partition plate method. The results indicated that VOCs produced from mint and lemongrass could be explored with an additive effect for the management of F. oxysporum f.sp. lycopercisi along with other Integrated Disease Management (IDM) practices.

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Section: Antifungal Activity Of Vocsupporting

confidence: 80%

Antifungal volatiles from medicinal herbs suppress Fusarium oxysporum f. sp. lycopersici

T¹,

As²,

Nakkeeran³

et al. 2021

J. Entomol. Zool. Stud.

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“…These fungal pathogens are more susceptible to the antagonistic activity of the volatiles exhibited by the basidiomycetes (Petre et al 2017). Futher more, Sangeetha (2018) also demonstrated a similar result on the effect of headspace volatiles produced by Chinese caterpillar mushroom (Ophiocordyceps sinensis) that strongly inhibited the mycelia growth of F. oxysporum f. sp. lycopersici and F. oxysporum f. sp.…”

Section: Fig 2 Inhibition Potential Of Macrobasidiomycetes Volatiles Against F Oxysporum Fsp Lycopercisisupporting

confidence: 54%

Enhancing Multinutrients Delivery and Seed Coating with Biodegradable Based Electrospun Nanofibre

Thangaraj¹,

Subbiah²,

Uthandi³

et al. 2022

MAJ

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Several macrobasidiomycete fungi have potential biological properties naturally to combat fungal diseases. F.oxysporum f.sp. lycopersici is a soilborne ascomycetous fungus, which causes disease in several vegetable crops. The present study aimed to explore the antifungal activity of VOCs produced by several macrobasidiomycete fungi against F. oxysporum f.sp. lycopersici. The VOCs emitted by macrobasidiomycete fungi were demonstrated by the inverted sealed plate assay against the target pathogen. Among the mushroom isolate tested in vitro, the VOCs exhibited from Coprinus cinereus inhibited 70 % mycelial growth of F. oxysporum f.sp. lycopersici, followed by Ganoderma lucidum (60.28 %) and Lentinus edodes (35.28 %). In addition to inhibition of the pathogen, the headspace volatiles emitted by the effective isolate were trapped by Tenax column and subjected to GC-MS analysis. A total of 15 and 25 VOCs were identified from C. cinereus and G. lucidum, respectively. Among them, Alfa copaene showed a peak area percentage of 7.82 (14.99 RT) in Coprinus cinereus. followed by 2 undecanone. Similarly, trichloromethane and 1- pentanol produced from G. lucidum, showed high relative abundance of 71.5 per cent at 9.84 RT and 70.3 per cent at 2.69 RT, respectively. The VOCs produced by macrobasidiomycete fungi could possess antimicrobial activities against the fungal pathogen. These volatile compounds may be explored as a novel biocontrol agent against soil borne pathogens of vegetable crop.

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“…Dynamic headspace (purge and trap) and static headspace sampling are two analytical methods that can be applied to extract and concentrate volatiles from the air surrounding a sample (Kusano et al, 2016; Park et al, 2017; Sangeetha et al, 2018). In static headspace sampling, the metabolic volatiles in the sample extract are allowed to equilibrate with air in air‐tight customized glassware.…”

Section: Methodologies For Metabolite Extractionmentioning

confidence: 99%

“…As a consequence, volatile profiles for isolation, detection and identification are highly and markedly dependent on the sample handling, sampling methodology and sample extraction approaches as well as on the chemical properties of the volatile present in the sample extract. As has been reported in metabolomics investigations(Kusano et al, 2016;Park et al, 2017;Sangeetha et al, 2018), no single analytical approach can provide a comprehensive profile of all the volatile and non-volatile compounds with very low concentrations (e.g., hormones and flavouring agents). A combination of selective broad-spectrum profiling approaches that entail targeted techniques to analyse and characterize important volatile and non-volatile metabolites is required.…”

mentioning

confidence: 99%

Disease diagnostic strategies and emerging analytical metabolomics techniques for waterborne microbial pathogens in vegetable crops—A critical review

et al. 2023

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A significant number of people around the world suffer from illnesses that are related to the consumption of unsafe vegetable crops. Early identification of waterborne microbial pathogens affecting vegetable crops can be an important step towards the control of disease. Microbial pathogens secrete extracellular metabolic volatiles or molecular biomarkers that affect the yield and quality of vegetable crops. These secreted compounds can be investigated using several applied analytical techniques, such as metabolomics, proteomics and other ‘omics’ tools. Currently, it is possible to use these approaches to characterize a broad range of specialized metabolites present in vegetable crops affected by waterborne and soilborne microbial pathogens. Such approaches may be used to determine molecular biomarkers, which can subsequently be exploited for the diagnosis of microbial pathogens that affect vegetable crops. Molecular biomarkers are involved in complex pathogenic biological processes involving mechanisms of pathogenesis and could be of great interest in the field of metabolomics. This review investigates disease diagnostic strategies and emerging analytical metabolomics techniques for waterborne microbial pathogens in vegetable crops. These will be vital in building comprehensive and more reliable data regarding the nature of secreted proteins and other specialized metabolites in vegetable crops and will lead to an increased understanding of the interactions between the host and pathogenic oomycetes, fungi, bacteria and viruses.

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Effect of Headspace and Trapped Volatile Organic Compounds (VOCs) of the Chinese Caterpillar Mushroom, Ophiocordyceps sinensis (Ascomycetes), against Soil-Borne Plant Pathogens

Cited by 7 publications

References 0 publications

Antifungal volatiles from medicinal herbs suppress Fusarium oxysporum f. sp. lycopersici

Antifungal volatiles from medicinal herbs suppress Fusarium oxysporum f. sp. lycopersici

Enhancing Multinutrients Delivery and Seed Coating with Biodegradable Based Electrospun Nanofibre

Disease diagnostic strategies and emerging analytical metabolomics techniques for waterborne microbial pathogens in vegetable crops—A critical review

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