Effect of Gynura procumbens Extracts on Anti-Proliferative Activity and its Associated Morphological Changes of Human Glioblastoma multiforme Cell Line (U-87)

Abstract

Objective: Gynura procumbens (Lour.) Merr is a well-known traditional herb and is widely used for traditional medicine by human. Previous clinical studies have shown the benefit effect of this leaves with inflammation, high blood pressure and others. This study aims to investigate the effect of methanol, ethanol, and ethyl acetate extracts of Gynura procumbens on U-87 cell line, human Glioblastoma multiforme cell line. Methods: In the present study, effect of methanol, ethanol and ethyl acetate extracts on U-87 cell line was determined by MTT-based anti-proliferative assay and any significant changes to the cells towards apoptotic changes was observed under a light microscope. Results: The ethanol and methanol extracts of Gynura procumbens was found to have high anti-proliferative effect on U-87 cell line with IC 50 less than 20µg/ml. By comparing IC 50 value, ethanol and methanol extracts of Gynura procumbens had high potential for anti-proliferative effect on U-87 cell line. Therefore, ethanol and methanol extracts can be a potential anti-proliferative effect on U-87 cell line. Conclusion: To the best of our knowledge, this is the first study of exploring the effect of Gynura procumbens extracts using three different polarity (methanol, ethanol, ethyl acetate) effect on U-87 cell line, Human Glioblastoma multiforme cells.

Root-shoot ratio and its relationships with physiological characteristics, growth and biomass yield of Gynura procumbens under different shade levels and plant density

“…Meanwhile, RSR, after 30 days of transplanting, started to grow slowly. This result parallels Qi et al 13 's findings, which reported that plants allocated more biomass to roots in the seedling stage or due to climatic conditions, soil fertility, and management practices 5,43,44 . Results of the present experiments showed that the highest net photosynthetic rate of G. procumbens was 13.89 µmol m -2 s -1 at 30% shade than 12.38 µmol m -2 s -1 at 0% shade (full sunlight) (Table 1).…”

“…It is widely distributed in Africa 1,2 and tropical regions of Southeast Asia and China 3,4 . Non-toxic leaves have extensive pharmacological properties, including anti-diabetic, anti-hypertensive, antioxidant, and vasorelaxant activity 5 . The medicinal benefits of G. procumbens are related to its bioactive compounds, such as saponins, flavonoids, and terpenoids 4,6 .…”

Gynura procumbens Standardised Extract Reduces Cholesterol Levels and Modulates Oxidative Status in Postmenopausal Rats Fed with Cholesterol Diet Enriched with Repeatedly Heated Palm Oil

“…Merr locally known as “pokok sambung nyawa” is commonly found in Malaysia, Indonesia, Thailand, Vietnam, and China. It is traditionally used to treat various diseases including fever, rashes, constipation, kidney disease, and cancer [17, 18]. Previous pharmacological studies have shown that G. procumbens possesses anti-inflammatory, antihypertensive, antihyperlipidaemic, antioxidative, and cardioprotective properties [19].…”

An in vivo and in silico evaluation of the hepatoprotective potential of Gynura procumbens: A promising agent for combating hepatotoxicity

“…[18][19][20][21][22][23][24][25][26][27][28]. Among these plants, Gynura procumbens is an extraordinary herb widely available in the southeast region of the world, such as Thailand, Malaysia, China, Vietnam, Bangladesh, and Indonesia [29]. It is a small (around 1-3 m long) flowering plant that belongs to the Asteraceae family.…”

“…Moreover, the most recent revelation of the antioxidant properties of this plant gives a clear and precise idea to treat various diseases by reducing free radical production [33,[43][44][45][46][47]. For this reason, the Chinese name of this plant is "Pokok Sambung Nyawa," the meaning of which is "prolongation of life" [37,48]. Furthermore, certain findings have provided significant evidence demonstrating the promising hepatoprotective potentials of G. procumbens, which has piqued the interest of researchers to investigate further the protective effects of G. procumbens against specific types of hepatotoxicity [24,49].…”