Effect of growth hormones on some antioxidant parameters and gene expression in tomato

Abstract

Bioregulators have a great effect on vital processes of plant growth and development. Known plant bioregulators include Naphthalene acetic acid (NAA), Indole-3-butyric acid (IBA) and Indole-3-acetic acid (IAA). Natural or synthetic plant bioregulators are organic compounds that affect the physiological processes in the plant, either to control some of these processes or to modify them. For example these bioregulators can affect the nature of the process, either by accelerating or decelerating plant growth, rates of maturation and also by altering the behavior of the plants or their products. Also, enhancement of important nutrients in human diet could be achieved by bioregulators. This study uses the model crop plant Tomato (Lycopersicon esculentum). Tomato is affected by a group of bioregulators, this group contains compounds which are powerful antioxidants in vitro. The current study aims to find out the effect of some plant bioregulators (IAA, IBA and NAA) on tomato growth, total protein content and enzyme activities of ascorbate peroxidase (APX), superoxide dismutase (SOD) and catalase (CAT). This study also investigates the effect of the above mentioned bioregulators on the level of RNA expression for SOD, CAT and TPX1 genes. The analytical quantification of target gene expression showed the induced effect of NAA on SOD expression and reducing effect of the other bioregulators (IAA and IBA) on CAT and TPX1 expression. However, at the protein level, we foundthat IBA and IAA caused a minor effect on total protein content while a significant effect was recorded on the total protein level using NAA. Upon measuring the enzyme activity of ascorbate peroxidase and catalase, we found that both the exogenous NAA and IBA stimulated ascorbate peroxidase activity in tomato while there was no considerable difference detected in IAA treated plants. Also, there was no considerable difference detected in catalase activity of all bioregulator-treated plants compared with the control.

Molecular Responses of Maize Shoot to a Plant Derived Smoke Solution

“…Antioxidants are mostly expressed to cope with stressed situations and their expression also has positive effects on the activation of enzymes related to plant growth [58,59]. In the present study, plant-derived smoke is behaving as a growth promoter and thus did not affected ascorbate peroxidase enzyme levels (Figure 7A).…”

Effect of different stress treatments on mature green tomatoes (Solanum lycopersicum) to enhance fruit quality

“…Sucrose is one of the compatible solutes that get accumulated in plants, particularly under drought or salt stress [9]. In this study, treatment of plants with NaCl led to the highest increase in sucrose (3.2x).…”

“…Global production of tomato was estimated at over 153 million metric tonnes in 2009 [8]. Tomato consumption has been linked to reduced risks of cancer especially prostate cancer and reduced occurrence of cardiovascular diseases [9]. This is because tomato contains high amounts of antioxidants such as vitamins A, C and E, carotenoids, flavonoids and phenolic acids [10].…”

Foliar Aspersion of Salicylic Acid Improves Phenolic and Flavonoid Compounds, and Also the Fruit Yield in Cucumber (Cucumis sativus L.)

“…With the increase in the concentration of SA, the concentration of total phenolic compounds and total flavonoids was improved, obtaining the highest concentration of these compounds at a dose of 0.5 mM, exceeding the control treatment by 37.5% and 45.46%, respectively. El-Gaied et al (2013) observed that phytochemical compounds are increased in response to a biochemical stress in cell suspensions caused by the increase in SA [27]. It has also been found that SA induces the production of hydrogen peroxide (H 2 O 2 ), which stimulates a greater activity of phenylalanine ammonium lyase, responsible for the synthesis of phenolic compounds [28].…”