Effect of Growth Factors on Cell Proliferation and Epithelialization in Human Skin

Bhora, Faizullah Y.; Dunkin, Brian J.; Batzri, Shmuel; Aly, Hamdy M.; Bass, Barbara L.; Sidawy, Anton N.; Harmon, John W.

doi:10.1006/jsre.1995.1160

Cited by 155 publications

(106 citation statements)

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“…To address this question, we have taken advantage of short-term organ and explant cultures of adult human skin. These experimental systems have emerged as powerful models for the study of wound healing (30,(44)(45)(46), as they permit the use of pharmacologic inhibitors that could be deleterious in vivo. Moreover, as opposed to cell culture studies, organ cultures constitute a fully differentiated tissue that retains dermal-epidermal interactions, and is subjected to trauma very similar to a normal cutaneous wound.…”

Section: Discussionmentioning

confidence: 99%

“…Known wound healing-related events that have been observed in organ culture include increased collagenolytic (47) and plasminogen activator activity (48), enhanced keratinocyte migration (49), and induction of proteoglycans such as tenascin (50). More recently, epidermal outgrowth from porcine (30) and human (46) skin explants have been shown to be responsive to exogenous EGF. To our knowledge however, no previous studies have explored the effects of EGFR blockade on these responses.…”

Section: Discussionmentioning

confidence: 99%

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Heparin-binding ligands mediate autocrine epidermal growth factor receptor activation In skin organ culture.

Stoll¹,

Garner²,

Elder³

1997

J. Clin. Invest.

103

122

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Exogenous EGF and TGF-␣ accelerate wound healing, but treatment effects are often modest. Using short-term human skin organ culture, we found that autocrine EGF receptor activation could account for this observation. Amphiregulin and heparin-binding EGF-like growth factor (HB-EGF) transcripts were rapidly and markedly induced, whereas EGF and TGF-␣ mRNAs were undetectable or only slightly increased. Vascular permeability factor and keratin 6 transcripts were also strongly induced, albeit with a Ն 3 h delay relative to HB-EGF and amphiregulin. All four transcripts were upregulated in actual healing skin wounds, HB-EGF and keratin 6 being the most prominent. The highly EGF receptor-specific tyrosine kinase inhibitor PD153035 strongly inhibited induction of all four transcripts in organ culture, as well as release of immunoreactive HB-EGF into the medium. These effects were confirmed using the anti-EGF receptor mAb 225 IgG. Neither PD153035 nor 225 IgG was toxic to keratinocytes, as judged by calcein-AM uptake. PD153035 completely abrogated the proliferative phase of keratinocyte outgrowth in skin explant cultures, whereas it had no effect on the antecedent migratory phase. Based on these results, we conclude that EGF receptor activation by highly inducible, keratinocyte-derived heparin-binding ligands is an important mechanism for amplification and transmission of the cutaneous wound healing signal. ( J.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Heparin-binding ligands mediate autocrine epidermal growth factor receptor activation In skin organ culture.

Stoll¹,

Garner²,

Elder³

1997

J. Clin. Invest.

103

122

View full text Add to dashboard Cite

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“…It has been reported that bFGF is expressed in dermal cells and keratinocytes, and is involved in cell proliferation and epithelialization in skin [29, 30, 31]. In addition, KGF is produced by mesenchymal cells and acts specifically on epithelial cells [32, 33].…”

Section: Discussionmentioning

confidence: 99%

Cell Proliferation and Lipid Formation in Hamster Sebaceous Gland Cells

et al. 2002

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Background: The development of sebocytes and lipogenesis are known to be dependent on androgens. However, it is not fully understood whether growth factors are involved in the regulation of cell proliferation and lipid formation in sebaceous glands. Objective: This study was designed to evaluate the effect of growth factors such as epidermal growth factor (EGF), transforming growth factor α (TGF-α), basic fibroblast growth factor (bFGF) and keratinocyte growth factor (KGF) on cell proliferation and lipogenesis in cultured hamster sebocytes. Methods: Cell proliferation and intracellular lipid accumulation in these hamster sebocytes treated with growth factors were examined. Results: EGF, TGF-α and bFGF augmented the proliferation of hamster sebocytes for 8 days in a time- and dose-dependent manner. However, KGF had no effect on their proliferation. Moreover, the accumulation of intracellular lipids consisting mainly of triglycerides was suppressed in EGF-, TGF-α-, bFGF- and KGF-treated hamster sebocytes. Conclusion: EGF, TGF-α and bFGF, but not KGF, have mitogenic activity on hamster sebocytes, and all these growth factors act as antilipogenic factors. Moreover, it is likely that the formation of intracellular lipid droplets is independent of cell proliferation in hamster sebocytes.

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“…This deposition pattern seals the wound cells. (7), (8), (9) The use of plant extract in the external management of wound is becoming widely acceptable because there is a growing interest in the clinical practice of wound management with the use of chemical componentimpregnated dressing. (10) The result of the experiment showed that the methanol extract of Napoleona Vogelii leaves has a wound healing activity, although this was not dose dependent.…”

Section: Discussionmentioning

confidence: 99%

Wound Healing Effect of Methanol Leaf Extract of Napoleona Vogelii Family (Lecythidaceae)

J.C¹

2013

IOSR-JDMS

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Effect of Growth Factors on Cell Proliferation and Epithelialization in Human Skin

Cited by 155 publications

References 0 publications

Heparin-binding ligands mediate autocrine epidermal growth factor receptor activation In skin organ culture.

Heparin-binding ligands mediate autocrine epidermal growth factor receptor activation In skin organ culture.

Cell Proliferation and Lipid Formation in Hamster Sebaceous Gland Cells

Wound Healing Effect of Methanol Leaf Extract of Napoleona Vogelii Family (Lecythidaceae)

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