Effect of growth factors on the differentiation of chick precardiac mesoderm in vitro

Yamazaki, Yoshihito; Hirakow, Reiji

doi:10.1007/bf00360525

Cited by 2 publications

(1 citation statement)

References 18 publications

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“…These include the developing myocardium and endocardial cushion tissue, smooth muscle progenitor cells in the developing arteries, the notochord, myotome, mesenchymal cells in the limbs, the coelomic epithelia, and chondro-ossification tissue. A number of studies have shown that TGFβ stimulates the tissue induction of mesoderm and of mesoderm derivatives during vertebrate development (Kimelman and Kirschner 1987;Rosa et al 1988;Asashima et al 1990;Yamazaki and Hirakow 1994;Dickson et al 1995). A comparison of the TGFβ3 expression patterns seen in chick and mouse embryos reveals a number of similarities between these two species, e.g., (1) TGFβ3 is expressed mainly in mesoderm-derived tissues, and (2) no detectable signal for TGFβ3 is found in developing nervous tissue or in morphogenetically inactive epithelia (in the process of differentiation, Table 1; Millan et al 1991).…”

Section: Discussionmentioning

confidence: 95%

Expression of TGFβ3 RNA during chick embryogenesis: a possible important role in cardiovascular development

Yamagishi

Nakajima

Nakamura

1999

Cell and Tissue Research

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We examined the temporal and spatial expression pattern of transforming growth factor (TGF)-beta3 RNA during chick embryogenesis from stage 6 to stage 33 (Hamburger and Hamilton scale) by using in situ hybridization. During cardiogenesis, TGFbeta3 mRNA was first expressed in the premyocardium at stage 8 and thereafter it was localized in endocardial cushion tissue and the ventricular myocardium until the end of embryogenesis. During the formation of the major arteries, mRNA for TGFbeta3 was found in smooth muscle progenitor cells, but not in endothelium. In addition, TGFbeta3 mRNA was detectable in various mesoderm-derived tissues, such as the notochord, myotome, pleura, peritoneum, mesenchymal cells in the limb, and developing bone. These results suggest that TGFbeta3 plays an important role in the development of the cardiovascular system and of other mesodermal derivatives during chicken embryogenesis.

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Section: Discussionmentioning

confidence: 95%

Expression of TGFβ3 RNA during chick embryogenesis: a possible important role in cardiovascular development

Yamagishi

Nakajima

Nakamura

1999

Cell and Tissue Research

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Expression of smooth muscle alpha-actin in mesenchymal cells during formation of avian endocardial cushion tissue: A role for transforming growth factor β3

et al. 1997

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During early cardiac morphogenesis, outflow tract (OT) and atrio‐ventricular (AV) endothelial cells differentiate into mesenchymal cells, which have characteristics of smooth muscle‐like myofibroblasts, and which form endocardial cushion tissue, the primordia of valves, and septa in the adult heart. During this embryonic event, transforming growth factor β3 (TGFβ3) is an essential element in the progression of endothelial‐transformation into mesenchyme. TGFβs are known to be a potent inducer for mesodermal differentiation and a promoter for differentiation of endothelial cells into smooth muscle‐like cells. Using a monoclonal antibody against smooth muscle‐specific alpha‐actin (SMA), we examined the immunohistochemical staining of this form of actin in avian endocardial cushion tissue formation. To determine whether TGFβ3 initiates the expression of SMA, the pre‐migratory AV endothelial monolayer was cultured with or without chicken recombinant TGFβ3 and the expression of SMA was examined immunochemically. Migrating mesenchymal cells expressed SMA beneath the cell surface membrane. These cells showed a reduction of endothelial specific marker antigen, QH1. Stationary endothelial cells did not express SMA. The deposition of SMA in the mesenchymal tissue persisted until the end of the fetal period. Pre‐migratory endothelial cells cultured in complete medium (CM199) that contained TGFβ3 expressed SMA, whereas cells cultured in CM199 alone did not. At the onset of the endothelial‐mesenchymal transformation, migrating mesenchymal cells express SMA and the expression of this form of actin is upregulated by TGFβ3. The induction of the expression of SMA by TGFβ3 is one of the initial events in the cytoskeletal reorganization in endothelial cells which separate from one another during the initial phenotypic change associated with the endothelial‐mesenchymal transformation. Dev. Dyn. 209:296–309, 1997. © 1997 Wiley‐Liss, Inc.

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Effect of growth factors on the differentiation of chick precardiac mesoderm in vitro

Cited by 2 publications

References 18 publications

Expression of TGFβ3 RNA during chick embryogenesis: a possible important role in cardiovascular development

Expression of TGFβ3 RNA during chick embryogenesis: a possible important role in cardiovascular development

Expression of smooth muscle alpha-actin in mesenchymal cells during formation of avian endocardial cushion tissue: A role for transforming growth factor β3

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