Effect of Growth Conditions on the Production, Composition and Viscosity of Xanthomonas campestris Exopolysaccharide

Tait, M. I.; Sutherland, Ian W.; Clarke-Sturman, A.J.

doi:10.1099/00221287-132-6-1483

Cited by 38 publications

(25 citation statements)

References 29 publications

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“…Xanthan was extracted after 9 days of culture. The procedures for extraction and determination of the viscosity and composition (glucuronic, acetic, and pyruvic acid contents) of xanthan were performed as described by Tait et al (41).…”

Section: Methodsmentioning

confidence: 99%

A Xanthomonas campestris pv. campestris protein similar to catabolite activation factor is involved in regulation of phytopathogenicity

et al. 1990

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A DNA fragment from Xanthomonas campestris pv. campestris that partially restored the carbohydrate fermentation pattern of a cya crp Escherichia coli strain was cloned and expressed in E. coli. The nucleotide sequence of this fragment revealed the presence of a 700-base-pair open reading frame that coded for a protein highly similar to the catabolite activation factor (CAP) of E. coli (accordingly named CLP for CAP-like protein). An X. campestris pv. campestris clp mutant was constructed by reverse genetics. This strain was not affected in the utilization of various carbon sources but had strongly reduced pathogenicity. Production of xanthan gum, pigment, and extracellular enzymes was either increased or decreased, suggesting that CLP plays a role in the regulation of phytopathogenicity.

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Section: Methodsmentioning

confidence: 99%

A Xanthomonas campestris pv. campestris protein similar to catabolite activation factor is involved in regulation of phytopathogenicity

et al. 1990

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“…Colorimetric microanalyses were performed for O-acetyl groups, pyruvate ketals and uronic acids as described by Tait et al (1986). Following enzymatic degradation, the diffusible oligosaccharide products were separated from limit (nondegradable) material by dialysis and evaporated to dryness under reduced pressure.…”

Section: Analytical Proceduresmentioning

confidence: 99%

Bacteriophage and associated polysaccharide depolymerases – novel tools for study of bacterial biofilms

Hughes

Sutherland

Clark

et al. 1998

Journal of Applied Microbiology

180

134

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Bacteriophage for three representative strains of Gram‐negative biofilm bacteria have proved to be of widespread occurrence. Lytic bacteriophage have been isolated from local sewage for the bacterium 1·15, an exopolysaccharide (EPS)‐producing pseudomonad found originally as a component of biofilms in a local river, and for two Enterobacter agglomerans strains from industrial biofilms. Representative examples of all three bacteriophage possess a relatively low burst size and on solid media, exhibit very large plaques surrounded by a wide halo (5–20 mm) indicative of polysaccharide depolymerase action. The bacteriophage are thus similar to other viruses for EPS‐producing bacteria in inducing the synthesis of enzymes degrading the polymers which occlude the bacterial cell surface. In each preparation, the polysaccharase activity was associated both with sedimented phage particles and with the supernate of bacterial lysates. The enzymes have been partially purified and used to prepare polysaccharide digests in which the major products from each polysaccharide are the presumed repeat units of the polymers or oligomers of these. The soluble phage enzymes each degrade their substrate by acting as endo‐glycanohydrolases. The phage and their associated enzymes thus provide very useful highly specific tools for studies of biofilms incorporating the bacterial host strains. Their potential applications in studies on bacterial biofilms are discussed.

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“…Tait et al (1986) observed that high C : N and C : P ratios in the medium were associated with high levels of polymer production. However, no difference was found between the amount of polymer extracted from planktonic cells in the culture outflow in the three media (extraction and quantitation methods were as described by Bonthrone et al, 2000).…”

Section: Time (H) Mean Depth (µM) Maximum Depth (µM) Minimum Depth (µM)mentioning

confidence: 96%

Effect of nutrient limitation on biofilm formation and phosphatase activity of a Citrobacter sp.

et al. 2002

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A phosphatase-overproducing Citrobacter sp. (NCIMB 40259) was grown in an air-lift reactor in steady-state continuous culture under limitation of carbon, phosphorus or nitrogen. Substantial biofilm formation, and the highest phosphatase activity, were observed under lactose limitation. However, the total amount of biofilm wet biomass and the phosphatase specific activity were reduced in phosphorus-or nitrogen-limited cultures or when glucose was substituted for lactose as the limiting carbon source. Scanning electron microscopy (SEM), transmission electron microscopy (TEM) and confocal laser scanning microscopy (CLSM) showed differences in cell and biofilm morphology in relation to medium composition. Electron microscopy suggested that the differences in biofilm formation may relate to differential expression of fimbriae on the cell surface.

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Effect of Growth Conditions on the Production, Composition and Viscosity of Xanthomonas campestris Exopolysaccharide

Cited by 38 publications

References 29 publications

A Xanthomonas campestris pv. campestris protein similar to catabolite activation factor is involved in regulation of phytopathogenicity

A Xanthomonas campestris pv. campestris protein similar to catabolite activation factor is involved in regulation of phytopathogenicity

Bacteriophage and associated polysaccharide depolymerases – novel tools for study of bacterial biofilms

Effect of nutrient limitation on biofilm formation and phosphatase activity of a Citrobacter sp.

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