Effect of growth conditions on enzyme activities, intracellular kinetics and biomass yields of a new RuMP-type methylotroph (T15)

Bussineau, C. M.; Keuer, Thomas A.; Chu, I‐Ming; Papoutsakis, Eleftherios T.

doi:10.1007/bf00282150

Cited by 9 publications

(1 citation statement)

References 23 publications

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“…Methane monooxygenase from several species of methanotrophs has been shown to insert an oxygen atom into a wide range of substrates (Anthony, 1975;Anthony, 1982;Bussineau and Papoutsakis, 1988;Bussineau and Papoutsakis, 1986;Bussineau et al, 1987). These include carbon monoxide, ammonia, n-alkanes, n-alkenes, dimethyl and diethyl ether, alicyclic and aromatic hydrocarbons.…”

Section: Introductionmentioning

confidence: 99%

Fossil Energy Biotechnology

1993

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Portions of this document may be illegible in electronic image products.Images are produced from the best avaiiable original document. EXPERT PANELThe following panel of distinguished scientists evaluated the research needs for fossil energy biotechnology applicable to the programs of the Department of Energy. This project was conducted under ,contract to Consultec Scientific, Inc., Knoxville, TN from the US.

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Section: Introductionmentioning

confidence: 99%

Fossil Energy Biotechnology

1993

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Mixed substrate‐metabolism in an obligate methanol utilizer: Chemostat studies

Samuelov

1989

Biotech & Bioengineering

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In Pseudomonas C, an obligate RMP-methylotroph, externally added formic acid and multi-carbon intermediates are co-metabolized in the obligate presence of methanol. Cell yield and methanol-carbon conversion to cell mass are optimal in a chemostat culture growing on a mixture of energy sources and methanol as the sole limiting carbon source. Growth on methanol as the sole, obligate energy source and an additional carbon source (e.g. pyruvate) is less efficient. Under these energy limited conditions, the cyclic oxidation pathway is predominantly operated.

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Growth of the obligate methylotroph Methylobacillus flagellatum under stationary and nonstationary conditions during continuous cultivation

Baev

Schklyar

Chistoserdova

et al. 1992

Biotech & Bioengineering

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The growth characteristics of a chemostat culture of the obligate methylotrophic bacterium Methylobacillus flagellaturn have been determined. Steady-state cultures growing at a rate of 0.73-0.74 h-', equal to the maximal growth rate, were obtained under oxyturbidostat cultivation conditions. The response of a chemostat culture to a pulse increase of methanol concentration was studied. It was shown that slow and rapidly growing cultures of M. flagellaturn responded differently to pulse methanol addition. The growth characteristics of slow-growing cultures decreased after methanol addition compared to those of stationary chemostat cultures. The growth characteristics of rapidly growing cultures were practically unchanged with and without pulse methanol addition.

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Effect of growth conditions on enzyme activities, intracellular kinetics and biomass yields of a new RuMP-type methylotroph (T15)

Cited by 9 publications

References 23 publications

Fossil Energy Biotechnology

Fossil Energy Biotechnology

Mixed substrate‐metabolism in an obligate methanol utilizer: Chemostat studies

Growth of the obligate methylotroph Methylobacillus flagellatum under stationary and nonstationary conditions during continuous cultivation

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