Growth of the obligate methylotroph <i>Methylobacillus flagellatum</i> under stationary and nonstationary conditions during continuous cultivation

Baev, Mark V.; Schklyar, N. L.; Chistoserdova, Ludmila; Chistoserdov, A. Y.; Polanuer, B. M.; Tsygankov, Y. D.; Sterkin, V E

doi:10.1002/bit.260390614

Cited by 10 publications

(9 citation statements)

References 23 publications

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“…(i) Primary oxidation of methanol and methylamine. M. flagellatus exhibits high growth rates on methanol or methylamine (up to 0.73 h Ϫ1 [2,3,7]) and possesses high activities of MDH and MADH, respectively (7,37). The genome analysis revealed the presence of a gene cluster predicted to encode MDH and accessory proteins (mxaFJGIR SACKLD; Mfla2034 to Mfla2044) similar to the clusters characterized in other methylotrophs (9,52,54).…”

Section: Resultsmentioning

confidence: 89%

“…The strain was isolated in the early 1980s from a metropolitan sewer system (24) and selected as a prospective industrial strain due to its high growth rates on methanol, high tolerance to methanol and formaldehyde, high biomass yield, and high coefficient of conversion of methanol into biomass (2,3,7,24). Derivatives of the strain have been successfully generated, aimed at commercial production of value-added compounds (6,21,41).…”

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confidence: 99%

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Genome of Methylobacillus flagellatus , Molecular Basis for Obligate Methylotrophy, and Polyphyletic Origin of Methylotrophy

et al. 2007

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Along with methane, methanol and methylated amines represent important biogenic atmospheric constituents; thus, not only methanotrophs but also nonmethanotrophic methylotrophs play a significant role in global carbon cycling. The complete genome of a model obligate methanol and methylamine utilizer, Methylobacillus flagellatus (strain KT) was sequenced. The genome is represented by a single circular chromosome of approximately 3 Mbp, potentially encoding a total of 2,766 proteins. Based on genome analysis as well as the results from previous genetic and mutational analyses, methylotrophy is enabled by methanol and methylamine dehydrogenases and their specific electron transport chain components, the tetrahydromethanopterin-linked formaldehyde oxidation pathway and the assimilatory and dissimilatory ribulose monophosphate cycles, and by a formate dehydrogenase. Some of the methylotrophy genes are present in more than one (identical or nonidentical) copy. The obligate dependence on single-carbon compounds appears to be due to the incomplete tricarboxylic acid cycle, as no genes potentially encoding alpha-ketoglutarate, malate, or succinate dehydrogenases are identifiable. The genome of M. flagellatus was compared in terms of methylotrophy functions to the previously sequenced genomes of three methylotrophs, Methylobacterium extorquens (an alphaproteobacterium, 7 Mbp), Methylibium petroleiphilum (a betaproteobacterium, 4 Mbp), and Methylococcus capsulatus (a gammaproteobacterium, 3.3 Mbp). Strikingly, metabolically and/or phylogenetically, the methylotrophy functions in M. flagellatus were more similar to those in M. capsulatus and M. extorquens than to the ones in the more closely related M. petroleiphilum species, providing the first genomic evidence for the polyphyletic origin of methylotrophy in Betaproteobacteria.Methylotrophy is the metabolic capacity to grow on reduced carbon compounds containing no COC bonds, such as methane, methanol, methylated amines, etc. (1, 39). While the role of methanotrophs in the reduction of global emissions of methane has been well recognized (27), less attention has been paid to nonmethanotrophic methylotrophs as participants in the global carbon cycle. However, recent models estimate methanol emissions into the atmosphere at 82 to 273 teragrams (Tg) year Ϫ1 (with living plants as the major source [19,25]), putting methanol emission on a scale similar to that of methane emissions (approximately 600 Tg year Ϫ1 [36]) and pointing toward the global role of nonmethanotrophic methanol utilizers. While no global modeling has been attempted to characterize the production of methylated amines, they are known to be abundant in marine and freshwater environments and represent dynamic constituents of not only carbon but also nitrogen global cycles (44). So far, only nonmethanotrophic methylotrophs have been implicated in utilizing methylated amines (1, 39).Methylobacillus flagellatus strain KT utilizes methanol and methylated amines as the sole sources of carbon and energy and is classifi...

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Section: Resultsmentioning

confidence: 89%

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confidence: 99%

Genome of Methylobacillus flagellatus , Molecular Basis for Obligate Methylotrophy, and Polyphyletic Origin of Methylotrophy

et al. 2007

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“…This bacterium has a high maximum growth rate (µ l 0n74) and a high methanol conversion coefficient (50 %, Baev et al, 1992). These properties make M. flagellatus an ideal producer for the biotechnology industry.…”

Section: Introductionmentioning

confidence: 99%

Organization of threonine biosynthesis genes from the obligate methylotroph Methylobacillus flagellatus The GenBank accession numbers for the aspC-hom-thrC-thyA gene cluster and thrB gene sequences from M. flagellatus reported in this paper are L78665 and L78666, respectively.

et al. 1999

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The genes encoding aspartate kinase (ask), homoserine dehydrogenase (hom), homoserine kinase (thrB) and threonine synthase (thrC) from the obligate methylotroph Methylobacillus flagellatus were cloned. In maxicells hom and thrC directed synthesis of 51 and 48 kDa polypeptides, respectively. The hom, thrB and thrC genes and adjacent DNA areas were sequenced. Of the threonine biosynthesis genes, only hom and thrC were tightly linked in the order hom-thrC. The gene for thymidylate synthase (thyA) followed thrC and the gene for aspartate aminotransferase (aspC) preceded hom. All four genes (aspC-hom-thrC-thyA) were transcribed in the same direction. mRNA analysis indicated that hom-thrC are apparently transcribed in one 7 5 kb transcript in M. flagellatus. Promoter analysis showed the presence of a functional promoter between aspC and hom. No functional promoter was found to be associated with the DNA stretch between hom and thrC. The thrB gene encoded an unusual type of homoserine kinase and was not linked to other threonine biosynthesis genes.

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“…Showing Jaccard cluster analysis of methylotrophic community based on 16S rDNA amplicons from samples obtained from five locations of Ghazipur landfill site Delhi Methylobacillus flagellatus strain KT utilizes methanol and methylated amines as the sole sources of carbon and energy and is classified as an obligate methylotroph. The strain was isolated from a metropolitan sewer system and selected as a prospective industrial strain due to its high growth rates on methanol, high tolerance to methanol and formaldehyde, high biomass yield, and high coefficient of conversion of methanol into biomass (Baev et al, 1992;Chistoserdova et al, 2007). Methylotrophy is the metabolic capacity to grow on reduced carbon compounds such as methane, methanol, methylated amines, etc.…”

Section: Denaturing Gradient Gel Electrophoresismentioning

confidence: 99%

Molecular detection of Methylotrophs from an Indian landfill site and their potential for biofuel production

2017

Global NEST Journal

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Emission of CH4 from landfills is a major cause of concern as CH4 is twenty four times more potent than CO2, as a greenhouse gas. However, landfills also harbor a group of bacteria called methanotrophs, which can oxidize CH4. They can be used for in situ bioremediation to reduce methane emissions. They can also be used for production of methanol or renewable diesel, utilizing methane in natural gas or biogas. Methanotrophs are a subgroup of methylotrophs. We used molecular techniques for detection of methylotrophs in samples from a landfill in New Delhi. We could detect five methylotrophs. Isolation and efficiency in methanotrophy of these bacteria is undergoing now.

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Growth of the obligate methylotroph Methylobacillus flagellatum under stationary and nonstationary conditions during continuous cultivation

Cited by 10 publications

References 23 publications

Genome of Methylobacillus flagellatus , Molecular Basis for Obligate Methylotrophy, and Polyphyletic Origin of Methylotrophy

Genome of Methylobacillus flagellatus , Molecular Basis for Obligate Methylotrophy, and Polyphyletic Origin of Methylotrophy

Organization of threonine biosynthesis genes from the obligate methylotroph Methylobacillus flagellatus The GenBank accession numbers for the aspC-hom-thrC-thyA gene cluster and thrB gene sequences from M. flagellatus reported in this paper are L78665 and L78666, respectively.

Molecular detection of Methylotrophs from an Indian landfill site and their potential for biofuel production

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